Isolation and Selection of Antagonistic Microbes for Biological Control of Zoysiagrass Large Patch Disease

Abstract

Abstract.A large patch disease caused by Rhizoctonia solani AG2-2 (IV) is a serious problem in Korean lawngrass (Zoysia japonica) sites including golf courses and sports fields in Korea. Antagonistic microorganisms against R. solani AG2-2 (IV) were isolated from various forest and crop soil sources in Southern Korea. Among the 61 isolates, I-009, FRIN-001-1, and YPIN-022 strains showing dramatic inhibition of the mycelial growth of R. solani AG2-2 (IV) in the pairing culture were selected as the most potential antagonistic microorganisms for this study. Based on the 16s RNA sequence comparison, I-009 and FRIN-001-1 isolates were identified as Bacillus spp., while YPIN-022 isolate belongs to the genus Pseudomonas. The greater inhibition (clear) zone between two edges of the selected and pathogenic microbes ranged from 11 to 15 mm in three selections, but the others averaged to 7 mm out of 30 mm distance. In another antifungal test using culture filtrate, those three isolates represented a range of 51.7 to 63.5% suppression potential. The selected isolates also inhibited significantly the stem-segment colonization by R. solani AG2-2 (IV) in vivo test by 28.1%, 43.0%, and 23.7% when inoculated with I-009, FRIN-001-1, and YPIN-022, respectively. The highest antagonistic activity for the large patch disease was demonstrated by the isolate FRIN-001-1, which will be useful for developing a bio-pesticide against Rhizoctonia.

Biocontrol of Large Patch Disease in Zoysiagrass (Zoysia japonica) by Bacillus subtilis SA-15: Identification of Active Compounds and Synergism with a Fungicide

“…Bacillus spp. are PGPRs that have been isolated from various sources such as organic matter and soil and are known to inhibit the growth of R. solani [17,18]. Specifically, B. subtilis SA-15 has a strong antifungal activity against various phytopathogens that decompose organic matter, including carbohydrates and proteins [12].…”

Isolation and Selection of Functional Microbes for Eco-friendly Turfgrass Management in Golf Course from Livestock Manure Compost

“…그러나 골프장의 잔디밭 은 잔디생육 과정에서 유기물 집적층인 대취층이 발달하 게 되며 (Ham et al, 2014), 이로 인해 토양의 물리성이 악 화되거나, 발병으로 잔디생육 및 품질 감소의 원인이 되기 도 한다 (Park et al, 1998). 이러한 문제를 해결하기 위해서 는 잔디 식재토양의 유기물 분해 활성과 잔디 병원균에 대 한 항균 활성을 갖는 미생물을 선발하여 잔디관리에 이용 하는 것이다 (Lim et al, 2011;Ma et al, 2013 …”

Growth of Creeping Bentgrass by Application of Compound Fertilizer Containing Microbes

“…효모균이나 유산균을 함유하는 미 생물 비료의 시비 또한 잔디 뿌리의 생육을 향상시킴으로 써 잔디의 생육과 품질이 증가되었다 (Kim et al, 2008a(Kim et al, , 2008bKim et al, 2010). 또한 유용미생물은 잔디 밭의 토 양 중 유기물을 분해하거나 ) 잔디의 근권 에 서식하는 주요 병원균에 대해 길항성을 나타내어 잔디 밭의 병 발생을 억제함으로써 (Lo et al, 1996;Ma et al, 2013) 잔디 생육과 품질을 향상시키기도 한다. 이는 근권에 서식 하는 미생물이 대사 과정에서 auxin이나 항균 활성 물질을 생성하거나 불용성 인산을 가용화하기 때문이다 .…”