Antifungal activity of some actinomycetes isolated from Riyadh soil, Saudi Arabia: An evaluation for their ability to control Alternaria caused tomato blight in green house pot trial)

Ara, Ismet

doi:10.5897/ajar12.398

Cited by 8 publications

(2 citation statements)

References 37 publications

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“…Five clinically yeast species, obtained from the Moroccan Coordinated Collections of Micro-organisms in the National Center for Scientific and Technical Research Morocco (CNRST), were used as test strains (Table 1). The selection of actinomycete strains with antifungal activity was realized by agar diffusion method in Sabouraud medium at 28°C (Ismet et al, 2012).…”

Section: Selection Of Actinomycete Isolates Producing Antifungal Subsmentioning

confidence: 99%

Novel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric Soil

Nafis¹,

Oubaha²,

Elhidar³

et al. 2018

OnLine Journal of Biological Sciences

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The aim of our study was to screen Moroccan actinomycete isolates able to produce nonpolyenic antifungal metabolites in order to contribute to limiting the problem of fungal infections emergence in particular mycotic diseases and to discover known antifungal families, especially nonpolyenic drugs. 480 isolates were tested against 5clinically pathogenic Candida species. Several methods have been used to study the polyenic or non-polyenic nature of the antifungal molecules produced by Actinobacteria (i) The study of the antibacterial activity (the bacterial plasma membrane is devoid of sterols); (ii) The screening of the antimicrobial activity of resistant strains to polyenic antifungals essentially Candida tropicalis R2 and Pythium irregular; (iii) The inhibition of antifungal activity by exogenous ergosterol addition in the culture medium and (iv) The UV-Visible light spectrophotometric analysis of the crude extracts of the actinomycete isolates. Among all isolate tested, only 60 (29 %) showed an antifungal activity against all test microorganisms used. Six active isolates meet all the selection criteria and produced nonpolyenic antifungal metabolites. The taxonomic study of the promising isolate Z26, using morphological, physiological and molecular characters, showed that it has 99,43% of similarity with Streptomyces phytohabitans but there were some differences in morphological characteristics. In addition, the chemical study, using chromatographic and spectroscopic techniques, of the bioactive substances produced in the Z26 isolate fermentation broth in NL300 culture medium allowed the determination of two macrolide derivatives with chemical structures C 62 H 100 O 24 (m/z 1251.6504 [M+Na] +) and C 68 H 110 O 26 (m/z 1365.7174 [M+Na] +). NMR experiments revealed that the active compounds were Novonestmycin A and B. The Novonestmycins A and B are for the first time produced by an actinomycete strain purely isolated from the Moroccan ecosystems.

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Section: Selection Of Actinomycete Isolates Producing Antifungal Subsmentioning

confidence: 99%

Novel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric Soil

Nafis¹,

Oubaha²,

Elhidar³

et al. 2018

OnLine Journal of Biological Sciences

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“…against the tea fungal plant pathogen Pestalotiopsis theae [35]. Previous research revealed that Streptomyces species culture extracts inhibited the growth of A. niger, A. flavus, and F. oxysporum [36,37]. However, current findings showed that Streptomyces sp.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 79%

GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Sholkamy

Palsamy

Raja

et al. 2023

Braz. arch. biol. technol.

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The main concern of today's agricultural production around the world is a cleaner and greener approach to crop production. Streptomyces species with distinct characteristics can be found in soil. The current research focuses on Streptomyces species isolated from marine sediment samples. To evaluate the morphology and biochemical properties of the isolated Streptomyces strain ukm1, it was grown in Starch Casein Nitrate (SCN) agar medium. Morphological, biochemical, and 16s rRNA analysis were performed on the strain. Streptomyces sp. was identified based on these findings. The production of bioactive metabolites by strain nkm1 was carried out in five different fermentative media. After 10 days of incubation, M6 medium was found to be efficient and was extracted with ethyl acetate. The MIC values revealed that the ethyl acetate extract inhibited the growth of plant pathogenic fungi such as Phytophthora palmivora (31.25 g/ml), Aspergillus flavus (15.62 g/ml), Rhizoctonia solani (31.25 g/ml), and Cladosporium herbarum (31.25 g/ml). The GC-MS analysis of the ethyl acetate extract revealed 26 components, the major volatile bioactive compound being nonadecane.

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Potential application of actinomycetes as natural fungicide

Yeng

Pahirulzaman

2021

IOP Conf. Ser.: Earth Environ. Sci.

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Fungal diseases in green leafy vegetables are commonly under-estimated. However, the application of agrochemical fungicide has raised multiple drawbacks such as the higher amount of chemical residues and intensified microbial community stress. There is an urgent need to establish a bio-control antifungal agent with minimal toxicity threat, which sourced from natural environment. This study aimed to isolate soil-borne fungi from soil underneath infected green leafy vegetables and identify the antifungal activities of actinomycete strains on the isolated fungi strain. Fungi were isolated from 3 soils collected underneath the infected green leafy vegetables. Actinomycete strains were screened for antifungal potential against selected fungi strains using agar plug assay. Forty-one fungal strains were isolated from the soil samples. Actinomycetes strain C.D6.5 exhibited the largest inhibition zone on five selected fungal strains, whereas actinomycetes strain C.KSJ 13.3 produced the broadest spectrum of antifungal activities. It can be concluded that actinomycetes strain have significant antagonistic activity against fungi and pure antifungal component can be extracted as an effective and environmental friendly bio-control agent on plant fungi.

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Antifungal activity of some actinomycetes isolated from Riyadh soil, Saudi Arabia: An evaluation for their ability to control Alternaria caused tomato blight in green house pot trial)

Cited by 8 publications

References 37 publications

Novel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric Soil

Novel Production of Two New Nonpolyenic Antifungal Macrolide Derivatives by Streptomyces Z26 Isolated from Moroccan Rhizospheric Soil

GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi

Potential application of actinomycetes as natural fungicide

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