Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

Tuyến, Đỗ Thị; Trung, Nguyen Thi; Thảo, Nguyễn Thị; Thanh, Nguyen Sy Le; Nguyen, Nguyen Phuong Dai; Tuyết, Nguyễn Thị; Cuong, Nguyen Tien; Chan, Sook Sin; Khoo, Kuan Shiong; Show, Pau Loke

doi:10.1016/j.ibiod.2022.105558

Cited by 9 publications

(2 citation statements)

References 40 publications

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“…2 , shows that B. subtilis inhibited the growth of G. trabeum by comparing the growth of the control mycelium which showed no inhibition. B. subtilis is able to inhibit the growth of G. trabeum because B. subtilis is able to produce several peptides that act as antibiotics and antifungals, such as subtilin, aterimin, bacitracin, subtilisin, mycobacillin, subsporin, ituirin, sericin, serelaxin, surfactin, bafilomycin, cyanide acid, and basillocine [ [24] , [25] , [26] , [27] ]. In addition, B. subtilis also produces macromolecular degradative enzymes that can destroy fungal cell walls, such as proteases (intracellular), and several extracellular enzymes secreted in the medium such as levansucrase, glucanase, amylase, xylanase, chitinase, and proteases [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum

Rizqi

Purnomo

Ulfi

2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum

Rizqi

Purnomo

Ulfi

2023

Heliyon

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“…The integration of antagonistic bacteria into biological control methods has enhanced their reliability, offering a promising avenue to reduce the usage of fungicides. The genus Bacillus is particularly noteworthy for its ability to produce a wide range of antibiotics, including both ribosomal and nonribosomal peptide antibiotics [34,35], and it finds applications in agriculture [36,37]. These bacteria are predominantly employed in postharvest processes to combat fruit rot caused by fungi.…”

Section: Detection Of Antagonistic Activities On Longan Fruitsmentioning

confidence: 99%

Biocontrol of Fruit Discoloring and Fruit Rot Disease in Longan (Dimocarpus longan) by Using Antagonistic Bacillus spp.

Suksakol,

Liamkraituan,

Teeraarunsiri

et al. 2024

Trends Sci

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Fruit discoloration and fruit rot disease in longan is a serious disease caused by the pathogenic fungi Pestalotiopsis oxyanthi and Lasiodiplodia pseudotheobromae. This study investigated the potential of antagonistic bacteria as an eco-friendly alternative to traditional fungicides for managing longan diseases caused by these fungal pathogens. Two bacterial isolates, UP-JLS050 and UP-JLS067, demonstrated significant inhibitory activity against the pathogenic fungi P. oxyanthi and L. pseudotheobromae, with high inhibitory activity. The bacterial strains were isolated from leaves and soil in a longan garden, and dual culture methods showed that the isolates UP-JLS050 and UP-JLS067 exhibited the highest inhibitory activity on the fungal pathogens, with percentage inhibitions of 72.67 ± 2.31 and 70.37 ± 1.28 %, respectively. Biochemical methods and molecular techniques, including sequencing of the 16S rDNA gene, were then used to identify the isolates. This confirmed their similarity to Bacillus subtilis and Bacillus amyloliquefaciens with 100 and 99 % identity, respectively. In vitro tests on longan fruit indicated that these antagonistic bacteria, administered at a concentration of 108 CFU/mL, significantly reduced disease incidence by up to 45 % after 9 days of incubation. The incubation of B. subtilis (UP-JLS050) and B. amyloliquefaciens (UP-JLS050) reduced disease incidence by 45 ± 5.77 and 32.5 ± 5.00 %, respectively. HIGHLIGHTS Fruit Discoloration and Rot in Longan: Caused by pathogenic fungi, namely Pestalotiopsis oxyanthi and Lasiodiplodia pseudotheobromae Antagonistic Bacteria for Disease Control: This study explores the potential of antagonistic bacteria as an eco-friendly alternative to traditional fungicides for managing longan diseases caused by these fungal pathogens Effective Inhibition: Two bacterial isolates, UP-JLS050 and UP-JLS067, demonstrated significant inhibitory activity against oxyanthi and L. pseudotheobromae, with inhibitions exceeding 70 % on PDA medium Identification of Bacterial Strains: Molecular techniques, including 16S rDNA gene sequencing, confirmed the identity of these isolates as subtilis and B. amyloliquefaciens Reduced Disease Incidence: In vitro tests on longan fruit indicated that these antagonistic bacteria, administered at a concentration of 108 CFU/ml, significantly reduced disease incidence by up to 45 % after 9 days of incubation GRAPHICAL ABSTRACT

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Gold Nanoparticles from Chenopodium botrys and Chenopodium ambrosioides as Bioreductants: In Vitro Antioxidant, Antibacterial, and an Eco-friendly Catalytic Potential in Dyes Degradation

Saba,

Khitab,

Jamila

et al. 2023

Arab J Sci Eng

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Antifungal activity of secondary metabolites purified from Bacillus subtilis isolated in Vietnam and evaluated on in vitro and in vivo models

Cited by 9 publications

References 40 publications

The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum

The effect of bacteria addition on DDT biodegradation by BROWN-ROT fungus Gloeophyllum trabeum

Biocontrol of Fruit Discoloring and Fruit Rot Disease in Longan (Dimocarpus longan) by Using Antagonistic Bacillus spp.

Gold Nanoparticles from Chenopodium botrys and Chenopodium ambrosioides as Bioreductants: In Vitro Antioxidant, Antibacterial, and an Eco-friendly Catalytic Potential in Dyes Degradation

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