Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi

Li, Yankai; Aioub, Ahmed A. A.; Lv, Bo; Hu, Zhaonong; Wu, Wenjun

doi:10.1016/j.indcrop.2019.02.009

Cited by 37 publications

(32 citation statements)

References 35 publications

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“…The antifungal activity of the obtained compounds WH01 and WH02 against V. mali was evaluated using mycelial growth method [25]. The antibiotics were dissolved with dimethylsulfoxide (DMSO) and diluted to appropriate concentration by melted PDA medium.…”

Section: Methodsmentioning

confidence: 99%

Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Wang

Tian

et al. 2019

Molecules

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Apple tree canker infected by Valsa mali var. mali is a serious and widely distributed disease in China. Saccharothrix yanglingensis Hhs.015 is an endophytic actinomycete isolated from cucumber roots, and it has been proven that this strain is a promising biocontrol agent on apple tree canker in previous studies. The aim of this study was to elucidate the active ingredients in its metabolites. Two pentaene macrolides, WH01 and WH02, were isolated from strain Hhs.015, and their structures were elucidated based on the extensive spectroscopic analysis. WH01 and WH02 were identified as fungichromin and 1′-deoxyfungichromin, among which WH02 is a novel compound. These two compounds showed strong in vitro and in vivo antifungal activity against V. mali. By comparison of the structures of hyphae cells treated by pure compound and fermentation broth, it has been proven that pentaene macrolides are the main active ingredients in the metabolites of strain Hhs.015. This is the first report on the antifungal activity of fungichromin and its analogs on V. mali, and the 28-member pentaene macrolides were also firstly isolated from the genus of Saccharothrix.

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Section: Methodsmentioning

confidence: 99%

Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Wang

Tian

et al. 2019

Molecules

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“…6,7 Scientists are shifting their research to the development of environment-friendly botanical fungicides with low toxicity, high efficiency, and easy degradation. [8][9][10][11] Many species of plants have been reported to be effective in preventing and controlling phytopathogenic fungi. 12,13 Phytophthora blight, caused by Phytophthora capsici, is a devastating soil-borne disease of many vegetable crops, which seriously threatens the production and quality of the crops.…”

Section: Introductionmentioning

confidence: 99%

Discovery of zeylenone from Uvaria grandiflora as a potential botanical fungicide

Dou

Xie

et al. 2021

Pest Management Science

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BACKGROUND: Botanical pesticides play an important role in organic agricultural practices and are widely used in integrated pest management (IPM). Uvaria grandiflora was mainly reported as traditional medicines and possessed antibacterial, antioxidant, and antiprotozoal activities. Therefore, important biological activities of U. grandiflora may suggest that they have the potential to be used as botanical pesticides.RESULTS: The extract of U. grandiflora exhibited broad-spectrum inhibitory activity toward phytopathogenic fungi and oomycetes, particularly against Colletotrichum musae and Phytophthora capsici, and its secondary metabolite zeylenone also displayed strong antifungal and anti-oomycete activities against phytopathogens. Particularly, half maximal effective concentration (EC 50 ) values of zeylenone against Phytophthora capsici and C. musae were 6.98 and 3.37 ∼g mL −1 , showing better inhibitory effects than those of commercial fungicides (azoxystrobin and osthole). Additionally, the pot experiments showed that the extract of U. grandiflora could effectively control Pseudoperonospora cubensis, Phytophthora infestans, Phytophthora capsici and Podosphaera xanthii. In the field experiment, 5% microemulsion of U. grandiflora extract exhibited 79.72% efficacy against cucumber powdery mildew at 87.5 g ha −1 on the 14th day after two sprayings, which was better than that of 21.5% trifloxystrobin and 21.5% fluopyram SC at 200.9 g ha −1 . Surprisingly, 5% microemulsion of U. grandiflora extract could promote cucumber growth significantly. Furthermore, the action mechanism analysis indicated that zeylenone may damage the cytoderm and affect energy metabolism of Phytophthora capsici.CONCLUSION: It is the first time that the extract of U. grandiflora and zeylenone have been discovered leading to broad application prospects in the development as botanical fungicides.

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“…19,20 For example, PSF and PSNW were considered stomach poisons against M. separata. 21,22 Periplocoside X (PSX) was used as a botanical pesticide against workers of the red imported fire ants (LD 50 = 0.116 mg/mL). 23 Additionally, further elaboration of these periplocosides' mode action has been conducted, with results showing that periplocosides could activate protease from the midgut of M. separata after being fed to pests.…”

Section: Introductionmentioning

confidence: 99%

“…Some of these periplocosides were regarded as potential botanical pesticides to control crop pests in China 19, 20 . For example, PSF and PSNW were considered stomach poisons against M. separata 21, 22 . Periplocoside X (PSX) was used as a botanical pesticide against workers of the red imported fire ants (LD 50 = 0.116 mg/mL) 23 .…”

Section: Introductionmentioning

confidence: 99%

Identification and mechanism of insecticidal periplocosides from the root bark of Periploca sepium Bunge

Tian

Zhang

et al. 2020

Pest Management Science

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BACKGROUND The Periploca sepium bark root (PSBR) has been regarded as a potential botanical insecticide because of its significant insecticidal activity of secondary metabolites. Several periplocosides were isolated from it as promising pesticides to control crop pests in agriculture. RESULTS In our research, two new periplocosides, along with four known periplocosides were isolated from PSBR. The names of new periplocosides were periplocoside T (PST) and periplocoside U (PSU) while another four periplocosides were known as follows: periplocoside A (PSA), periplocoside F (PSF), periplocoside E (PSE) and periplocoside D (PSD). All periplocosides were evalulated for insecticidal activity against 3rd Mythimna separata (Walker) and Plutella xylostella. The biometric data showed that periplocoside T, PSD and PSF had remarkable insecticidal activity against tested insects. Its values of LD50 were 1.31, 3.94 and 3.42 μg·lavare−1 against 3rd M. separata respectively, while the activity of those compounds against 3rd P. xylostella were 5.45, 12.17 and 13.95 μg·lavare−1, respectively. It was apparent after further study of the mechanism of action against M. separata was conducted that PST possessed the most significant insecticidal activity. The results of enzymatic activity displayed that powerful activation of tryptase, especially weak alkaline tryptase might be a dominant factor causing death of M. separata in vivo. CONCLUSION We herein report isolation and the mechanisms of action of insecticidal periplocosides, which established the fundamental development of natural agents to prevent pest damage to crops. © 2020 Society of Chemical Industry

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Antifungal activity of pregnane glycosides isolated from Periploca sepium root barks against various phytopathogenic fungi

Cited by 37 publications

References 35 publications

Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Investigation on the Antifungal Ingredients of Saccharothrix Yanglingensis Hhs.015, an Antagonistic Endophytic Actinomycete Isolated from Cucumber Plant

Discovery of zeylenone from Uvaria grandiflora as a potential botanical fungicide

Identification and mechanism of insecticidal periplocosides from the root bark of Periploca sepium Bunge

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