Antifungal activity of natural compounds against Candida species isolated from HIV-positive patients

Oro, Debora; Heissler, Andréia; Rossi, Eliandra Mirlei; Scapin, Diane; Malheiros, Patrícia da Silva; Boff, Everton

doi:10.1016/j.apjtb.2015.07.011

Cited by 15 publications

(16 citation statements)

References 14 publications

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“…The analyzed compounds via GC/MS and HPLC fingerprinting showed constituents in the plants at its crude level and these might be responsible for the inhibition of test isolates. It was mentioned that the complex nature of the phytochemical constituents might be responsible for varying level of activity on fungi isolates and grueling that is required in the determination of process for the target components that is responsible for the fungicidal activity (Balakumar et al, 2011;Ignacimuthu et al, 2011;Stevic et al, 2014;Oro et al, 2015). The fungicidal activity of the extracts might be because of the synergy or antagonistic activities between various elements in the plant extracts (Silva et al, 2010;Balouiri et al, 2016;Salah-Fatnassi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…A Modified method of Ngonda et al (2012), Mihai and Popa (2015) and Oro et al (2015) were adopted; Petri dishes of the concentrations of extracts that showed no visible fungal growth were seeded into freshly prepared PDA plate to assay for the fungicidal effect of the extracts. The plates containing the test organisms were incubated at 28°C.…”

Section: Determination Of Minimum Fungicidal Concentration (Mfc)mentioning

confidence: 99%

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Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Adeogun¹,

Adekunle²,

Adongbede³

et al. 2017

Not Sci Biol

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This study aimed to examine the preservative potential of Megaphrynium macrostachyum on fungi responsible for the deterioration of orange juice and corn Jell-O. The phytochemicals from plants' leaves were extracted with four solvents: acetone, aqueous, ethanol and hexane. The solvents were differently and tested against fungi isolated from orange juice and corn Jell-O using disc diffusion method. Phytochemical screening of the extracts from the leaves was carried out, and the most active extract was tested via GC-MS for the essential oils and HPLC fingerprinting. The toxicity test of the extracts against brine shrimp was carried out after exposure for 24 hours. The toxicity test showed that the extracts were non-toxic on the Brine Shrimps at LC 50 (379.21μg/ml and 107.21μg/ml for aqueous and ethanol extracts). The qualitative phytochemical test reported the presence of alkaloids, tannins, saponins, flavonoids, steroids and terpenoids in different extracts of the plant' leaves. The quantitative phytochemical determination of the most active extract revealed alkaloids with the highest contents of 107.48 mg/100 g. The GC-MS analyses of the fresh leaves of the plants revealed the presence of isodecane with the highest percentage at 15.56%. The GC-MS analyses of the dried leaves revealed isodecane with the highest percentage at 10.43%. The HPLC analysis revealed the presence of various phytochemical constituents in the dried leaves. This study has been able to establish the potency of Megaphrynium macrostachyum leaves on fungi associated with the spoilage of Citrus sinensis (orange) juice and Corn Jell-O ('Eko') which contribute to tremendous research towards the use and acknowledgment of natural antimicrobials for the preservation of food.

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Minimum Fungicidal Concentration (Mfc)mentioning

confidence: 99%

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Adeogun¹,

Adekunle²,

Adongbede³

et al. 2017

Not Sci Biol

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“…[2][3][4] In these population, Candida albicans (C. albicans) is the species that is commonly found for most of infections. 5 The most common etiology of OC in HIV/AIDS patients are C. albicans in Surabaya, Indonesia in around 54% of the subjects 2 and also in Malang, Indonesia. 6 While there was also some nonalbicans species that cause this infection, like C. glabrata, C. krusei, C. parapsilosis, and C. tropicalis.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antifungal Susceptibility Testing of Tea Tree Oil (TTO) 5% Compared with Nystatin against Candida sp. as Important Agent of Oral Candidiasis in HIV/AIDS Patients

Lydiawati

Listiawan

Murtiastutik

et al. 2020

BIKK

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Background: Oral candidiasis is the most common clinical manifestation in a person infected with HIV which has been linked with the severity of the disease. Candida albicans responsible for most oral candidiasis. The increased resistance to the available antifungal drugs can reduce the efficacy of therapy. Therefore, it will be crucial to discover novel antifungal agents. One of them is tea tree oil (TTO) or Melaleuca alternifolia which has a potent antifungal effect to inhibit the growth of Candida sp. Purpose: To evaluate the antifungal susceptibility of TTO 5% compared with nystatin against Candida sp. taken from HIV/AIDS patients. Methods: The study assessed the antifungal effect of TTO 5% and nystatin against 33 isolates of Candida sp. that was isolated from the oral cavity of 30 HIV/AIDS patients in Dr. Soetomo General Academic Teaching Hospital Surabaya, Indonesia. Antifungal susceptibility was evaluated by the disc diffusion method. The diameter of the inhibition zone was determined as the result of this study. Result: The most strains found in this study was Candida albicans (84.8%). The mean inhibition zone of nystatin against all strains was 23.24 mm whether it was 17.55 mm for TTO 5%. The inhibition zone of both TTO 5% and nystatin in Candida non-albicans were tended to be higher than in Candida albicans. It revealed that the inhibition zone of TTO 5% significantly lower than nystatin (P = 0.00; P < 0.05).Conclusion: Therefore, the antifungal susceptibility of nystatin was significantly higher than TTO 5% against Candida albicans and non-albicans.

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“…Other topical antifungal agents, such as clotrimazole, miconazole, and terbinafine, are less commonly administered due to their association with higher recurrence risk [ 9 ]. Recently, much attention has been paid to replace the synthetic anti-microbial medications with natural alternatives, which have less side effects and lower cost [ 10 , 11 ]. Kombucha tea is one of these natural alternatives, which is a popular traditional fermented drink in many countries.…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

Mahmoudi

Saeidi

et al. 2016

mazu-cmm

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Background and Purpose:Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate the antifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the patients with seborrheic dermatitis. Materials and Methods:A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL. Results:The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species, followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL. Conclusion:The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia.

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Antifungal activity of natural compounds against Candida species isolated from HIV-positive patients

Cited by 15 publications

References 14 publications

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

Chemical Composition, Toxicity and Antifungal Activities of Megaphrynium macrostachyum (K. Schum) Leaf Extract against Foodborne Fungi

In Vitro Antifungal Susceptibility Testing of Tea Tree Oil (TTO) 5% Compared with Nystatin against Candida sp. as Important Agent of Oral Candidiasis in HIV/AIDS Patients

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis

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