Reduction in the quality of fruits during storage has been a concern to the consumers and the effect can be felt on the economy of developing countries. Leaves of plants such as Canna indica, Megaphrynium macrostachyum and Thaumatococcus daniellii have been documented as food packaging materials in West Africa. Based on this, the quality of stored sweet orange juice was investigated using ethanolic extracts of leaves of C. indica, M. macrostachyum and T. daniellii to enhance the shelf life of the juice. The extracts were used to assess the quality of juice for 30 days using quantitative parameters such as total soluble solid, browning potential, pH, microbial analysis and turbidity at 4 oC and at room temperature (27-31 oC). The qualitative and quantitative phytochemical constituents of the extracts were determined. The extracts’ toxicity was determined using Brine shrimp. The quality assessment evidently revealed that the freshly squeezed orange juice with the extracts possess tolerable activity to enhance the shelf life of orange juice. The leaf extract of M. macrostachyum had the highest preservation rate on the juice after 30 days. The qualitative phytochemical screening revealed the presence of alkaloid, tannin, saponins, flavonoids, steroids and terpenoids in the three plants tested. The quantitative phytochemical analysis of the most active extracts in the three plants revealed that M. macrostachum had the highest contents of alkaloids (107.48 mg/g) and flavonoids (56.92 mg/g).The study showed that the extracts were non-lethal on Brine shrimp. This study ascertained the potential preservative qualities of the test plants for enhancing the shelf-life of orange juice.
This study was conducted to assess the retail market edibility quality of Citrullus lanatus fresh-cut fruits enhanced with the extracts from the leaves of Ocimum gratissimum. Analytical graded ethanol and hexane were used to extract O. gratissimum leaves differently. The fresh-cut fruits were dipped in the extract obtained from the leaves and reconstituted with Tween 80, fruits treated with Tween 80 and untreated fruits were used as controls. The treated fruits with the extracts, fruits treated with Tween 80 and fruits without treatment were analysed for quality test using the following parametres: carotenoids, ascorbic acids, total phenolic acid, pH, total soluble solids, microbial loads and moisture contents at day 0 and at an interval of 3, 5 and 9 days. The quantitative estimations of the phytochemicals in the extracts were determined and the constituents in the essential oil of the hexane extracts were established with the use of GC-MS system, and the data generated from the study were analysed with SPSS 20.0. This study showed that there was lower reduction in moisture content, pH, ascorbic acid and potential browning values of the treated fresh-cut fruits of C. lanatus, compared to higher reduction in untreated and tween 80 treated fresh-cut fruits during the storage intervals for nine days. The treated fresh-cut fruits had higher Brix values of TSS, total phenolic acid and carotenoids contents, compared with the untreated fresh-cut fruit, which was low during the period of storage for nine days. The microbial loads in the untreated fresh-cut fruit of C. lanatus were higher than what was obtained in the treated fresh-cut fruits of C. lanatus after nine days of storage. The yield of Alkaloids from ethanol and hexane extracts were 54.25±0.09 mg/ 100g and 51.86±0.06 mg/ 100g respectively. γ-Terpinene (17.21 %) and (E)-9-Octadecenoic acid (11.848 %) had the highest percentage composition of phytoconstituents present in the essential oil from Citrullus lanatus. This study was able to establish the preservation potential of Ocimum gratissiumum on fresh-cut fruits of Citrullus lanatus.
To determine the essential oils extracted from fresh and dried leaves of Artemisia afra using hydrodistillation (HD) and solvent free microwave extraction (SFME) methods and investigate the effects of the oils on Artemia salina. Methods: The essential oils were obtained from fresh and dried leaves of Artemisia afra using hydrodistillation and solvent-free microwave extraction methods. The compounds present in the oils were analysed by gas chromatography-mass spectrometry (GC-MS). The oils were assayed for hatchability and lethality activities on Artemia salina for 72 h. The lethal concentration (LC50) required to kill 50 % of the population of brine shrimp by each test oil was determined using a Probit regression analysis. Results: The most abundant compound was thujone (32.02 and 30.02 % in fresh leaf by HD and SFME methods, respectively) and in dried leaf (26.57 and 25.82 %, by HD and SFME methods, respectively). Mean hatchability success rate of all the oils was 70 % while lethality activity was 30 % after 72 h at the lowest concentration of the test oils. Half-maximal lethal concentration (LC50) on Artemia salina was 206.97 and 406.48 µg/mL of the oil from fresh leaf obtained by HD and SFME, respectively, while for the dried leaf, it was 277.18 and 669.30 µg/mL for the oil produced by HD and SFME, respectively. Conclusion: The phytoconstituents in each oil varied based on the method of extraction and the state of the leaf before and after extraction. Furthermore, the toxic activity of the oils against Artemia salina suggests that they may possess anticancer properties but this needs to be further investigated.
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