Antifungal Activity of Essential Oils against Candida albicans Strains Isolated from Users of Dental Prostheses

Abstract

Objective The objective of this study was to analyze the antifungal activity of citral, selected by screening natural products, against Candida albicans isolates from subjects who use dental prostheses. Methodology Screening of essential oils, including those from Mentha piperita L. (Briq), Origanum vulgare, and Zingiber officinale L., and the phytoconstituents citral and limonene, to select an appropriate natural product. Citral, which mediated the best antifungal response, was selected for biological assays. The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) for citral and nystatin were determined by the microdilution method. Micromorphological analyses, time-kill curve, and modulation tests were performed. Results The MIC and MFC of citral were established as 32 μg/mL, consistent with fungicidal activity. The clinical strains were resistant to nystatin. Citral caused micromorphological alteration in the strains. In the time-kill curve, the growth of the clinical strain was reduction in growth equal to 3 log10 colony-forming units per milliliter after exposure to the MIC and MIC × 2 of citral for 2 h. Citral did not modulate the resistance of the studied strains to nystatin. Conclusion This study revealed the potential of citral as a fungicidal agent and highlighted the resistance of clinical strains of C. albicans to nystatin.

Synergistic effects of Cinnamomum cassia L. essential oil in combination with polymyxin B against carbapenemase-producing Klebsiella pneumoniae and Serratia marcescens

“…The test was performed in duplicate and the means of the values obtained were used to classify the bacterium as sensitive (� 10 mm) or resistant (< 10 mm) [25]. The MIC of CCeo and trans-cinnamaldehyde (TC) were determined by broth microdilution, as described by Cavalcanti et al [26] and was determined by resazurin colorimetric assay. CCeo was diluted with distilled water and 0.5% Tween-80.…”

Chemical Variation and Pharmacological Properties ofDyssodia decipiensEssential Oil

“…Previous studies on the antimicrobial activity of limonene, revealed its strong anti-C. albicans activity and their mode of action. [12] The antifungal activity of linalool was comparable to that of verbenone. Published reports on the effectiveness of linalool on clinical isolates of C. albicans supported the weak inhibitory effects found in the present investigation.…”

Isoeugenol and Hybrid Acetamides against Candida albicans Isolated from the Oral Cavity

“…Strains ATCC-76485 and LM-4b were used to evaluate possible changes in the micromorphology of C. albicans cells exposed to isoeugenol. In this assay, microcultures were performed in a humid chamber using solid agar-cornmeal-Tween 80 (Difco ® , Sparks, MD, USA) [ 16 ]. Aliquots of 3 mL of liquid agar-cornmeal-Tween 80, homogenized with MIC, MIC × 2 and MIC × 4 of the test products, were poured into a glass slide and C. albicans were seeded and incubated at 35 °C ± 2 °C for 24–48 h.…”

“…In this context, medicinal plants represent a vast source of new pharmacologically active molecules for the treatment of fungal diseases [ 15 , 16 , 17 ]. Among the compounds isolated from plants, phenylpropanoids constitute a group of phytoconstituents derived from phenylalanine widely found in essential oils such as Syzygium aromaticum (clove), Myristica frangrans (walnut shown), Ocimum basilicum (basil) and Laurus nobilis (louro) oils [ 15 , 18 , 19 , 20 ], with isoeugenol being a representative of this class of molecules [ 21 , 22 ].…”