Antifungal activity of Bacillus amyloliquefaciens against Fusarium oxysporum f. sp. cubense race 1

Abstract

Introduction. Due to the absence of totally effective either economically viable chemical agents for the control of Fusarium wilt, the use of antagonistic microorganisms is of great interest since it could represent a more economically and ecologically sustainable alternative. Objective. To analyze the antifungal effect of the Bacillus amyloliquefaciens CCIBP-A5 strain against Fusarium oxysporum. Materials and methods. The work was carried out in the Laboratory of Applied Microbiology of the Instituto de Biotecnología de las Plantas, Cuba, between September 2017 and June, 2018. The in vitro and in vivo antifungal activity of its culture filtrate and cell against F. oxysporum has been assayed. Results. The results indicated that the metabolites present in the culture filtrate of B. amyloliquefaciens CCIBP-A5 significantly influenced the growth and morphology of the mycelium and the conidia. They also caused oxidative damage to the lipid molecules of F. oxysporum. In addition, this strain showed inhibitory effects on the development of the disease under controlled conditions. These aspects are key when selecting a bacterial candidate as a biological control agent. Conclusions. The results showed that the B. amyloliquefaciens CCIBP-A5 strain, isolated from Musa sp., had an in vitro antifungal effect against the vegetative and reproductive structures of Foc race 1 as well as on the Musa spp.-F. oxysporum interaction. This strain is suggested for the development of a bioproduct for Fusarium wilt management.

Effectiveness of Bio-agent Formulations on Suppression of Lupine wilt Caused by Fusarium oxysporum f. sp. lupini

“…To combat fungal infections, several species of Bacillus have been employed (Kamali et al 2019, Martín et al 2021. Numerous processes, including the creation of systemic resistance, the stimulation of host growth, and/or antibiosis, can be responsible for this.…”

“…It is known that Bacillus species lower the wilting index in plants inoculated with F. udum. The potential cause of the observed enhancement in plant development could be the pathogen-inhibiting properties of Bacillus species (Martín et al, 2021).…”

“…Similar to how B. pumilus is used as a biocontrol agent for Fusarium wilt, Pseudomonas species can likewise inhibit parasitic root infections by producing compounds that have biological activity (Akhtar and Siddiqui, 2007). Additionally, they produce siderophores, which limit the amount of iron available, the enzyme that regulates hormone levels, and antibiotics, which destroy pathogens (Martín et al 2021). According to Siddiqui et al (2007), applying Pseudomonads fluorescens and Bacillus spp.…”

Exploring Endophytic Bacteria from Artemisia spp. and Beneficial Traits on Pea Plants

“…The antagonistic activity of these endophytic bacteria against F. oxysporum has been well-documented. For instance, B. thuringiensis has been reported to inhibit the growth of F. oxysporum in peanut and onion plants [ 25 , 26 ], B. cereus strains in tomato [ 27 ], B. velezensis in cucumber [ 28 ], B. amyloliquefaciens in banana [ 29 ], and Pseudomonas fluorescens in watermelon [ 30 ]. These findings align with our results, highlighting the potential of these endophytic bacteria as effective biocontrol agents against root rot pathogens.…”

Growth inhibition of Fusarium oxysporum f. sp. nicotianae by two in vitro poisoning methods with fungicides of different toxicological groups

“…One of the most important limitations in production and quality is the presence of vascular diseases, mainly those caused by various species of Fusarium oxysporum (Olivares et al, 2021;Martín et al, 2021). In Cuba, 31 species of Fusarium associated with different commercial crops have been reported (López, 2004), although F. oxysporum f. sp.…”