Antifungal activity and mode of action of silver nano-particles on Candida albicans

Abstract

In this study, the antifungal effects of silver nano-particles (nano-Ag) and their mode of action were investigated. Nano-Ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. To elucidate the antifungal mode of action of nano-Ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (TEM) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (DPH), as a plasma membrane probe, were performed with Candida albicans. The results suggest nano-Ag may exert an antifungal activity by disrupting the structure of the cell membrane and inhibiting the normal budding process due to the destruction of the membrane integrity. The present study indicates nano-Ag has considerable antifungal activity, deserving further investigation for clinical applications.

Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp.

“…However, there were other deformations such as structure of the cell membrane and inhibiting normal budding process of both Rhizopus sp. and Aspergillus sp., probably due to the destruction of the membrane integrity (Narayanan and Park 2014;Kim et al 2009b). Almost similar observation was reported by Ouda (2014) who used copper and silver nanoparticles against two plant pathogens, Alternaria alternate and Botrytis cinerea.…”

Antimicrobial effects of silver zeolite, silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms

“…In contrast, silver, in the form of titanium silver alloy, is often used as material for dental implants, orthodontic brackets, wires and screws, showing no inhibitory effect against Lactobacillus acidophilus [31] . With regard to oral yeast, silver particles have demonstrated a remarkable antifungal activity toward C. albicans by attacking the plasma membrane resulting in the formation of pores, disrupting the membrane potential, inhibiting the budding process, and causing subsequent cell death [32] .…”

Photobiological synthesis of noble metal nanoparticles using Hydrocotyle asiatica and application as catalyst for the photodegradation of cationic dyes

“…Another possibility recommended is the release of Ag ? ions from the nanoparticles, which enhance the bactericidal properties of nanoparticles [56].…”

“…exists between the pH of 3.5-5.0 (k max = 617 nm), the protonated species MGH ? exists below pH 2 (k max = 255 nm) and at pH value above 8 it is colorless due to the formation of carbinol base [56]. To see the effect of pH on degradation of MG, the reaction is also studied at pH 9 ( Figure S9).…”