Antifungal activity against postharvest fungi by extracts from Colombian propolis

Abstract

Recebido em 10/7/08; aceito em 24/4/09; publicado na web 22/9/09 ANTIFUNGAL ACTIVITY AGAINST POSTHARVEST FUNGI BY EXTRACTS FROM COLOMBIAN PROPOLIS. The aims of the present study were to evaluate the antifungal properties of Colombian propolis extracts against Colletotrichum gloeosporioides and Botryodiplodia theobromae, and to isolate and identify the main constituents from the active extracts. Therefore, propolis samples were thoroughly extracted with n-hexane/methanol (EPEM), dichloromethane, ethyl acetate, and methanol. Experimental results indicated that mycelial growth of all selected microorganisms was reduced in culture media containing EPEM and dichloromethane fractions. Furthermore, through antifungal bioassay-guided fractionation, three known labdane-type diterpenes: isocupressic acid (1), (+)-agathadiol (2) and epi-13-torulosol (3) were isolated as the main constituents from the active fractions.

Efficacy Evaluation of Iraqi Propolis Against Gray Mold of Stored Orange Caused by Penicillium digitatum

“…Mello et al (2006) suggested that the antifungal activity of propolis maybe due to the changes in the cell wall that leading to increasing of volume and membrane rupture. Previous study found three compound isocupressic acid, (+)-agathadiol and epi-13-torulosol that have antifungal activity against the tested fungi on culture medium by reduce radial mycelium grow (Meneses et al, 2009). Ozcan et al (2003) also reported that the methanolic propolis extract had large scale effect to inhibition mycelial growth against many phytopathogenic fungi in culture medium.…”

The in vitro antifungal evaluation of a commercial extract of Chilean propolis against six fungi of agricultural importance

“…Therefore, independent of whether the protective chemical variability is maintained, the inhibitory activity of propolis is associated with the synergy of its components, consistent with Garedew et al (2004), Meneses et al (2009) and Petrova et al (2010), and it has not been shown experimentally that only a single component has greater activity than the total extract (Agüero et al, 2010). However, Treutter (2006) discussed the significance of flavonoids in the protection and resistance of plants to phytopathogens, particularly fungi, and phenolics and flavonoids are proposed to be the main antimicrobial components of propolis.…”

“…The control of phytopathogenic fungi by propolis has been proposed by Hegazi and Abd El Hady (2002) who evaluated the use of Egyptian propolis to control nine postharvest fungi and reported successful inhibition of the fungi, with minimum inhibitory concentration values ranging between 1.2-3.6 mg mL . In other countries, samples of the local propolis have been evaluated by Őzcan et al (2004), Quiroga et al (2006), Giovanelli (2008), Meneses et al (2009), andÖzdemir et al (2010) who obtained interesting results against phytopathogenic fungi. However, the development of a propolis extract for use as an agricultural fungicide has not been given much attention (Hegazy and Abd El Hady, 2002;Quiroga et al, 2006) in Chile, and data regarding its action against important economical agricultural fungi are limited.…”

Performance of Sirex noctilio ’s biocontrol agent Deladenus siricidicola , in known and predicted hosts

“…For example, the terpenoid torulosol was among the major compounds that differentiated hybrids and P. taeda in both standing and felled trees: torulosol and its derivatives have been identified in extracts that show antifungal activity (Meneses et al, 2009). The majority of compounds we detected in our samples were non-volatile oleoresins, composed mostly of monoterpenes and diterpene resin acids, which can present both a physical and chemical defence system against invading pathogens and insects.…”