Antifungal activities of silver and selenium nanoparticles stabilized with different surface coating agents

Abstract

BACKGROUND: Extensive and growing use of different chemical pesticides that affect both the environment and human health raises a need for new and more suitable methods to deal with plant pathogens. Nanotechnology has enabled the use of materials at the nanoscale with exceptional functionality in different economic domains including agricultural production. This study aimed to evaluate antifungal potential of selenium nanoparticles (SeNPs) and silver nanoparticles (AgNPs) stabilized with different surface coatings and characterized by different surface charge on plant pathogenic fungi Macrophomina phaseolina, Sclerotinia sclerotiorum and Diaporthe longicolla.RESULTS: AgNPs were coated with three different stabilizing agents: mono citrate (MC-AgNPs), cetyltrimethyl ammonium bromide (CTAB-AgNPs) and polyvinylpyrrolidon (PVP-AgNPs). SeNPs were coated with poly-L-lysine (PLL-SeNPs), polyacrylic acid (PAA-SeNPs), and polyvinylpyrrolidon (PVP-SeNPs). Seven different concentrations (0.1, 0.5, 1, 5, 10, 50 and 100 mg L −1 ) of nanoparticles were applied. All AgNPs and SeNPs significantly inhibited the growth of the tested fungi. Among the tested NPs, PVP-AgNPs showed the best inhibitory effect on the tested plant pathogenic fungi, especially against S. sclerotiorum. The similar inhibition of the sclerotia formation was observed for S. sclerotiorum treated with PLL-SeNPs. CONCLUSION:Obtained results provides new insights on fungicide effect of AgNPs and SeNPs stabilized with different coating agents on different plant pathogens. Further work should focus on detailed risk/benefit ratio assessment of using SeNPs or AgNPs in agriculture taking into account whole agroecosystem.

Antifungal Activity of a Composition of Selenium and Iodine Nanoparticles

“…These results agree with results of other authors. It is known that Se NPs have antifungal activity (Vrandečić et al, 2020;Shahverdi et al, 2020;Shakibaie et al, 2015;Eswarapriya and Jegatheesan, 2015;Joshi et al, 2019;Yip et al, 2014). It is also known that soluble compounds of iodine have antifungal activity against such fungi as Venturia inaequalis, Rhizoctonia solani, Fusarium oxysporum, Bipolaris sorokiniana, Fusarium moniliforme (Efimov et al, 2020), Candida albicans, C. parapsilosis, C. glabrata, C. tropicalis, C. lusitaniae, C. guilliermondii, C. krusei (Kondo et al, 2012, Oligoporus placenta, Gloeophyllum trabeum, Coniophora puteana, Trametes versicolor (Ihssen et al, 2014), other fungi and particularly against Acremonium species (Farrag et al, 2012).…”

“…Selenium NPs have antifungal activity against such fungi as Macrophomina phaseolina, Sclerotinia sclerotiorum, Diaporthe longicolla (Vrandečić et al, 2020), Malassezia sympodialis, Malassezia furfur, Aspergillus terreus (Shahverdi et al, 2020), Candida albicans, Aspergillus fumigatus, Aspergillus niger (Shakibaie et al, 2015;Eswarapriya and Jegatheesan, 2015), Pyricularia grisea, Colletotrichum capsici, Alternaria solani (Joshi et al, 2019), Trichophyton rubrum (Yip et al, 2014) and others.…”

Metal Nanoparticles as Novel Antifungal Agents for Sustainable Agriculture: Current Advances and Future Directions

“…In Table 1, the different extracts of plants and fungi that have been used to produce Ag nanoparticles are reported. In the case of the chemical route, several methods have been used to synthesize Ag nanoparticles, such as chemical reduction, sol-gel, and microemulsion [122][123][124][125][126][127][128][129][130]. To a lesser extent, physical methods have been used, such as high-voltage arc discharge and the irradiation method [131][132][133].…”

“…Ag nanoparticles ranging between 40 and 70 nm also show an inhibitory effect, destroying mycelium and spores and provoking the rupture of the membrane significantly [78,92,95,118,122,131]. Nevertheless, while the larger size has a good antifungal capacity, their penetration into the pathogen's membrane is slower, causing damage to mycelium and spores or the inhibition of fungal growth [110,121,129,132]. In Figure 3, severely damaged cell walls and hyphae with abnormal structures are shown in the presence of biosynthesized Ag NPs.…”

“…Therefore, different concentrations of Ag nanoparticles have been evaluated. Several studies have shown that the concentration of Ag nanoparticles has an important role in antifungal activity [83,113,129,130,137,146]. Interestingly, low concentrations showed effectiveness in the suppression of fungi.…”