Antifouling activity of enzyme‐functionalized silica nanobeads

Abstract

The amelioration of biofouling in industrial processing equipment is critical for performance and reliability. While conventional biocides are effective in biofouling control, they are potentially hazardous to the environment and in some cases corrosive to materials. Enzymatic approaches have been shown to be effective and can overcome the disadvantages of traditional biocides, however they are typically uneconomic for routine biofouling control. The aim of this study was to design a robust and reusable enzyme‐functionalized nano‐bead system having biofilm dispersion properties. This work describes the biochemical covalent functionalization of silica‐based nanobeads (hereafter referred to as Si‐NanoB) with Proteinase K (PK). Results showed that PK‐functionalized Si‐NanoB are effective in dispersing both protein‐based model biofilms and structurally altering Pseudomonas fluorescens biofilms, with significant decreases in surface coverage and thickness of 30.1% and 38.85%, respectively, while increasing surface roughness by 19 % following 24 h treatments on bacterial biofilms. This study shows that enzyme‐functionalized nanobeads may potentially be an environmentally friendly and cost effective alternative to pure enzyme and chemical treatments. Biotechnol. Bioeng. 2016;113: 501–512. © 2015 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

“…Synthesis of silica NPs was based on the Stöber method 55 and the subsequent functionalisation steps (amination, carboxylation) were carried out according to a procedure previously described. 56 For the synthesis of curc-NPs, 80 mg of carboxylatefunctionalised silica NPs, 47 mg of DCC, 14 mg of DMAP and 80 mg of curcumin were dispersed in 50 mL of dry DMF under inert atmosphere at 0 C. The mixture was stirred to room temperature and then le under agitation for 20 h. Yellowish coloured curc-NPs were recovered by centrifugation (9000 rpm, 20 min) and washed at least three times with water via centrifugation (Scheme 1). In the case where uorescently labelled NPs were desired, 1 mL of dye conjugate was added in the mixture of the adapted Stoeber reaction method.…”

Pectinases immobilization on magnetic nanoparticles and their anti-fouling performance in a biocatalytic membrane reactor

“…Most of the nanobeads used so far are being separated based on size exclusion. For instance, in the work of Zanoni et al, 21 a 500 nm Si-nanobeads with immobilized enzyme that was used to distract biolm growth were recovered using 0.45 mm lters. However, due to the presence of residual gels that could not be isolated from the Si-nanobeads using a mere ltration, the recycled Si-nanobeads exhibited reduced mechanical or enzymatic activity on the biolms.…”

“…As a result, authors suggested the need to develop an alternative and highly efficient recovery and cleaning methodology. 21 In this work, these challenges are resolved by using magneto-responsive nanobeads.…”

Combination of selected enzymes with cetyltrimethylammonium bromide in biofilm inactivation, removal and regrowth

“…Lequette et al (2010) combined seven proteases and polysaccharidases with CIP strategies against Pseudomonas fluorescens and Bacillus cereus biofilms. More recently, Zanoni, Habimana, Amadio, and Casey (2015) attempted to control P. fluorescens biofilms with Proteinase K immobilized on silica nanobeads. Meshram, Dave, Joshi, Dharani, Kirubagaran, and Venugopalan (2016) studied P. aeruginosa biofilm control by immobilizing alginate lyase on cellulose acetate membranes.…”