Antifibrotic effect of the ethyl acetate fraction of ciplukan (Physalis angulata Linn.) in rat liver fibrosis induced by CCI4

Abstract

Ciplukan (Physalis angulata Linn.) is a Solanaceae family species and contains various active compounds with diverse therapeutic potential. The goal of this investigation was to see if the ethyl acetate fraction of ciplukan had an antifibrotic impact on liver fibrosis. The oral administration of 20% carbon tetrachloride (CCl 4 ) twice weekly for 8 weeks was used to cause liver fibrosis. Four weeks following fibrosis induction, ciplukan ethyl acetate fractions of 1.11 mg (CPL-1) and 2.22 mg (CPL-2) were given orally. As a positive control group, vitamin E was used. When compared to the negative control, the ethyl acetate portion of 2.22 mg (CPL-2) lowered serum alanine aminotransaminase levels (83.95 ± 27.675 vs 175.23 ± 5.641, p-value < 0.05). Microscopic histopathological changes based on the better Metavir score (CPL-2 vs. negative control = 1.25 ± 1.893 vs. 3.50 ± 0.577; p-value < 0.05) and Ishak score (CPL-2 vs. negative control = 1.50 ± 1.000 vs. 4.75 ± 0.957 p-value < 0.05) were demonstrated. Overall, in rat liver fibrosis induced by CCl 4 , these findings suggest that the ethyl acetate fraction of ciplukan has an antifibrotic effect.

Physalis angulata Linn. as a medicinal plant (Review)

“…P. angulata L. is an effective acute anti-inflammatory agent and its potential action against chronic diseases, such as fibrosis, is also being investigated. Fibrosis is associated with diseases including the hepatitis virus, non-alcoholic fatty liver disease, chronic kidney diseases, idiopathic pulmonary fibrosis, pneumoconiosis and cystic fibrosis ( 36 ). Global disability-adjusted life-years in 2019 were significantly impacted by fibrosis-related disorders ( 36 ).…”

“…Fibrosis is associated with diseases including the hepatitis virus, non-alcoholic fatty liver disease, chronic kidney diseases, idiopathic pulmonary fibrosis, pneumoconiosis and cystic fibrosis ( 36 ). Global disability-adjusted life-years in 2019 were significantly impacted by fibrosis-related disorders ( 36 ).…”

“…Transforming growth factor β-1 induced HSC proliferation was also inhibited by physalin B. Furthermore, in vivo studies revealed that physalin B reduces hepatic injury, as measured by decreased aspartate aminotransferase and alanine transaminase (ALT) levels ( 36 ). Histopathological examination also demonstrated that physalin B could repair liver fibrosis ( 37 ).…”

“…Based on Dewi's research that P. angulata L. can therapeutically improve skin fibrosis, research was continued on other organs. Rohmawaty et al ( 36 ) conducted the research on liver of male adult Wistar rats that induced by carbon tetrachloride (CCl4) to perform liver fibrosis model.…”

“…A total of four weeks following fibrosis induction, P. angulata L. ethyl acetate fractions of 1.11 mg (CPL-1) and 2.22 mg (CPL-2) were administered orally. As a positive control group, vitamin E was used ( 36 ).…”