Antifibrotic Activity of Hyaluronidase Immobilized on Polyethylenoxide under Conditions of Bleomycin-Induced Pneumofibrosis

Abstract

Hyaluronidase immobilized on polyethylenoxide obtained by electron bean synthesis was administered intranasally and intravenously to C57Bl/6 mice after intratracheal bleomycin and the enzyme effects on the development of pneumofibrosis in animals were studied. Intranasal immobilized hyaluronidase prevented connective tissue growth in the lungs exposed to bleomycin and virtually did not modulate the infiltration of the alveolar and alveolar duct interstitium by inflammatory cells (lymphocytes, macrophages, neutrophils, plasma cells). The antifibrotic effect developed sooner after intranasal inoculation of immobilized hyaluronidase and was more pronounced than after intranasal native hyaluronidase. Intravenous injection of immobilized hyaluronidase did not modify the inflammatory process and deposition of collagen fibrils in the lung parenchyma in pneumofibrosis.

The Rise and Fall of Hyaluronan in Respiratory Diseases

“…Teder et al observed that CD44 −/− mice succumbed to unremitting inflammation following bleomycin lung injury, characterized by the accumulation of HA fragments at the site of tissue injury and impaired clearance of macrophages, neutrophils, and lymphocytes [ 84 ]. Dygai et al observed that the intranasal application of hyaluronidase, immobilized on polyethyleneoxide, did not modify the inflammatory process or deposition of collagen fibrils in the lung parenchyma [ 85 ]. Studies by others have substantiated, and expanded, these observations [ 86 – 92 ].…”