Antidiarrhoeal and antispasmodic activity of leaves of Syzygium cumini L. (Myrtaceae) mediated through calcium channel blockage

Monteiro, Fabio de S.; Carvalho, Antônio Felipe Silva; Marques, Elismar de Castro; Ribeiro, Rachel Melo; Borges, Antônio Carlos Romão; Borges, Marilene Oliveira da Rocha

doi:10.5897/ajpp2017.4868

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…For this, verapamil (1 µM) was employed as a positive control. This chemical has been identified as a calcium channel blocker for L-type dependent VGC channels [35]. Our results indicate that EoWs prevented calcium entry through the VGC channels and, as a result, calcium release from the endoplasmic reticulum.…”

Section: Discussionmentioning

confidence: 62%

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Aziz¹,

Marghich²,

Addi³

et al. 2022

Front. Biosci. (Schol Ed)

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With several medicinal and aromatic species, the Asteraceae family is one of the largest angiosperm families. The genus Warionia is represented in this family by only one species, Warionia saharae. In Moroccan traditional medicine, this species is widely used to treat gastrointestinal problems. Essential oil of this plant (EoWs) was studied for possible myorelaxant and antispasmodic activities to rationalize some of the traditional uses. In this investigation, hydrodistillation was used to obtain the essential oil from the aerial part of the dry plant extract (EoWs), which was then analyzed using gas chromatography coupled to mass spectrometry (GC/MS). The major compounds identified in the EoWs are nerolidyl acetate (21.44%), β-Eudesmol (19.47%), linalool (16.48%), 1-terpinene-4-ol (10.93%), and cineole (5.34%). EoWs is relatively safe in the case of acute intake up to 2 g/kg body weight of albino mice. The effect of EoWs on intestinal relaxation was investigated using rabbit and rat jejunal smooth muscle. We have noticed that EoWs produce a myorelaxation on basal rabbit jejunum's contractions in a concentration-dependent manner with a maximal effect at 30 µg/mL. This myorelaxation was not dependent on adrenergic receptors. When the rat jejunums were pre-contracted with 25 mM KCl or 10 µM Carbachol (CCh), EoWs had an antispasmodic action with an IC50 values of 15.76 ± 0.37 and 12.04 ± 0.30 µg/mL, respectively. Preliminary results showed that it is probable that our plant might act directly through the NO and guanylate cyclase signaling pathway and on muscarinic but not nicotinic receptors. The results reveal that the Essential oil of W. saharae appears to have an impact on intestinal relaxation in vitro conditions. This finding lends credence to the traditional usage of this plant to treat intestinal disorders.

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Section: Discussionmentioning

confidence: 62%

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Aziz¹,

Marghich²,

Addi³

et al. 2022

Front. Biosci. (Schol Ed)

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“…The results indicate that AEJO (0.1, 0.3, and 1 mg/ml) added in a cumulative fashion diminished the maximum tone induced by low K + , while demonstrating a weak inhibitory effect on high K + in a dose-dependent manner, thus indicating that the inhibitory effect probably passed through K + channel opening activity (15). All doses of AEJO completely inhibited the maximum response to CaCl 2 , similar to the effect of verapamil, a positive control known for blocking voltage-dependent L-type channels (19). Like non-competitive antagonists, AEJO blocks Ca 2+ channel pathways.…”

Section: Discussionmentioning

confidence: 75%

Antispasmodic and antidiarrheal effects of <i>Juniperus oxycedrus</i> L. on the jejunum in rodents

Amrani,

Karim,

Marghich

et al. 2024

J. Smooth Muscle Res.

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Functional bowel disorders (FBD) have a major potential to degrade the standards of public life. Juniperus oxycedrus L. ( J. oxycedrus ) (Cupressaceae) has been described as a plant used in traditional medicine as an antidiarrheal medication. The present study is the first to obtain information on the antispasmodic and antidiarrheic effects of J. oxycedrus aqueous extract through in vitro and in vivo studies. An aqueous extract of J. oxycedrus (AEJO) was extracted by decoctioning air-dried aerial sections of the plant. Antispasmodic activity was tested in an isolated jejunum segment of rats exposed to cumulative doses of drogue extract. The antidiarrheic activity was tested using diarrhea caused by castor oil, a transit study of the small intestine, and castor oil-induced enteropooling assays in mice. In the jejunum of rats, the AEJO (0.1, 0.3 and 1 mg/ml) diminished the maximum tone induced by low K + (25 mM), while it exhibited a weak inhibitory effect on high K + (75 mM) with an IC 50 =0.49 ± 0.01 mg/ml and IC 50 =2.65 ± 0.16 mg/ml, respectively. In the contractions induced by CCh (10 −6 M), AEJO diminished the maximum tone, similar to that induced by low K + (25 mM). with an IC 50 =0.45 ± 0.02 mg/ml. The inhibitory effect of AEJO on low K + induced contractions was significantly diminished in the presence of glibenclamide (GB) (0.3 µM) and 4-aminopyrimidine (4-AP) (100 µM), with IC 50 values of 1.84 ± 0.09 mg/ml. and 1.63 ± 0.16 mg/ml, respectively). The demonstrated inhibitory effect was similar to that produced by a non-competitive antagonist acting on cholinergic receptors and calcium channels. In castor oil-induced diarrhea in mice, AEJO (100, 200, and 400 mg/kg) caused an extension of the latency time, a reduced defecation frequency, and a decrease in the amount of wet feces compared to the untreated group (distilled water). Moreover, it showed a significant anti-motility effect and reduced the amount of fluid accumulated in the intestinal lumen at all tested doses. These findings support the conventional use of Juniperus oxycedrus L. as a remedy for gastrointestinal diseases.

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“…EH-FAB concentrations were used in multiples of three, with the maximum concentration being 729 µg/mL since it is the maximum concentration used in experiments with isolated organs. When the observed effect was larger than 50% at 729 µg/mL, it was sought the concentration that would provide a 0% effect (Monteiro et al, 2018(Monteiro et al, , 2020.…”

Section: Preparation Of Crude Extractmentioning

confidence: 99%

“…The concentration of EH-FAB that produced the maximum relaxing effect (E max ) was expressed as mean ± S.E.M. (Monteiro et al, 2018).…”

Section: Investigation Of the Effect Of Eh-fab In Isolated Rat Jejunummentioning

confidence: 99%

“…The antispasmodic activity is related to the ability of certain medications (extracts of plants, fractions, or isolated substances) to prevent or interrupt the painful and involuntary contraction (spasm) of the intestinal smooth muscle (Forster et al, 1980;Hani, 2014;Har & Croffie, 2017;Monteiro et al, 2018). To study that activity and its likely mechanism of action in intestinal smooth muscle, segments of isolated jejunum from rats can be used.…”

Section: Introductionmentioning

confidence: 99%

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Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

Monteiro

Costa

Martins³

et al. 2020

Rev. Ciênc. Farm. Básica Apl.

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Aim: Since other species of the Bignoniaceae Family presented of antispasmodic activity, it was decided, by chemotaxonomic criterion, to determine the antispasmodic activity of the leaves of Arrabidaea brachypoda. Methodology: the segments of the rat jejunum were suspended in glass vats containing specific saline solution, at an appropriate temperature, and after stabilization period, were stimulated by a contractile agent to observe the inhibitory or relaxing effect of EH-FAB. Results: EH-FAB showed the presence of 10 compounds, mainly rutin and it has an antispasmodic activity as it inhibits the phasic component and relaxes the tonic component of the contraction in isolated rat jejunum. To assess the mechanism of antispasmodic action, cumulative curves to the CCh were performed in which a non-competitive antagonism was observed, due to a displacement of the control curve to the right and reduction of the maximum contraction effect (E max). Afterward, the participation of the calcium and/or potassium channels was evaluated by increasing the extracellular potassium, and it was observed that the EH-FAB relaxed the rat jejunum, suggesting the participation of the Ca 2+ channels. To corroborate that hypothesis, the EH-FAB was tested against cumulative curves to Ca 2+ in a free depolarizing solution of Ca 2+ , and it was observed that there was a shift of the curve to the right with a reduction in E max. Conclusions: EH-FAB presents antispasmodic activity in isolated rat jejunum and it is suggested to block the influx of Ca 2+ through voltage-gated calcium channels, signaling the therapeutic potential for the treatment of colic and/or diarrhea.

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Antidiarrhoeal and antispasmodic activity of leaves of Syzygium cumini L. (Myrtaceae) mediated through calcium channel blockage

Cited by 12 publications

References 42 publications

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Antispasmodic and antidiarrheal effects of <i>Juniperus oxycedrus</i> L. on the jejunum in rodents

Hydroalcoholic extract of leaves of Arrabidaea brachypoda (DC.) Bureau present antispasmodic activity mediated through calcium influx blockage

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