Antidiarrheal, antimicrobial and antioxidant potentials of methanol extract of Colocasia gigantea Hook. f. leaves: evidenced from in vivo and in vitro studies along with computer-aided approaches

Abstract

Background Colocasia gigantea, locally named as kochu is well-known due to its various healing power. This research is to investigate the antidiarrheal, antimicrobial and antioxidant possibilities of the methanol soluble extract of Colocasia gigantea. Methods The antidiarrheal investigation was performed by using in vivo castor oil-induced diarrheal method whereas in vitro antimicrobial and antioxidant investigation have been implemented by disc diffusion and DPPH scavenging method respectively. Moreover, in silico studies were followed by molecular docking analysis of several secondary metabolites that were appraised with Schrödinger-Maestro v11.1 and Biovia Discovery Studio. Results The induction of plant extract (200 and 400 mg/kg, b.w, p.o) has minimized the castor oil mediated diarrhea by 16.96% (p < 0.01) and 38.89% (p < 0.001) respectively compared to control group. The methanol extract of C. gigantea showed mild sensitivity against almost all the tested strains but it shows high consistency of phenolic content and yielded 67.68 μg/mL of IC50 value in the DPPH test. In the PASS prediction, selected isolated compounds have demonstrated significant antidiarrheal and antimicrobial activity following the Lipinski drug rules which have ascertained efficacy with the compounds in molecular docking study. Conclusion The results of this scientific research reflects that the methanol soluble extract of C. gigantea is safe and may provide possibilities of alleviation of diarrhea along with being a potential wellspring of antioxidant and antimicrobial agents which can be considered as an alternate source for exploration of new medicinal products in near future.

Computer-aided Approaches to Support the Ethnopharmacological Importance of Dillenia pentagyna Roxb.: An In silico Study

“…Computational analysis or molecular docking is frequently used to estimate interactions between ligands and targets along with gaining the deeper insights into the biological actions of bioactive compounds (Alam et al, 2021b). It also offers further comprehensions into the potential mechanisms of action and binding manners within the enzyme binding pocket (Alam et al, 2021a).…”

Chemico-Pharmacological Screening of the Methanol Extract of Gynura nepalensis D.C. Deciphered Promising Antioxidant and Hepatoprotective Potentials: Evidenced from in vitro, in vivo, and Computer-Aided Studies

“…The animals were observed individually during the first 30 min after dosing, and special attention was given during the first 4 h, then observed periodically during the first 24 h to see any toxic effects in the animals. During the entire period of observation for 14 days, the animals were observed and monitored for any changes in behavior, body weight, urination, food intake, water intake, respiration, convulsions, tremor, temperature, and constipation, changes in eye and skin colors and mortality of the animals [ 40 ].…”

“…The stock solution was prepared by following the method used by Alam et, al. [ 40 ]. The stock solution was serially diluted to achieve the concentrations of 400 µg/mL, 200 µg/mL, 100 µg/mL, 50 µg/mL, 25 µg/mL, and 12.5 µg/mL.…”

“…Incubate the mixture at room temperature for 30 min in a dark place. Then the absorbance is measured at 517 nm against dilute extract solution in the solvent [ 40 ].…”

“…To prepare proteins, the nonpolar hydrogens and the Gasteiger charge were retained at their default settings. UCSF Chimera was also used to reduce all proteins to their lowest energy state and process them for further analysis keeping them in the normal residues in AMBER ff14sB and other residues in Gasteiger mode [ 40 ].…”