Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy

Sharma, Rohit; Amin, Hetal; Galib,; Prajapati, PK

doi:10.1016/s2221-1691(15)30173-8

Cited by 84 publications

(55 citation statements)

References 53 publications

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“…It is formulated as a hard gelatin capsule containing a dried and powdered mixture of six medicinal herbs reported for anti diabetic activity. This new herbal formula contains Phyllanthus amarus (aerial parts) [7], Tinospora cordifolia ( stems and leaves) [8] , Emblica officinalis (fruits) [9] and Eugenia jambolana (fruits) [10], Gymnema sylvestre (leaves) [11] and Cassia auriculata (flowers) [12]. The composition of herbs is given Table 1.…”

Section: Introductionmentioning

confidence: 99%

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Telapolu

Kalachavedu

Punnoose³

et al. 2018

BMC Complement Altern Med

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BackgroundType 2 Diabetes (T2D) is a polygenic disease requiring a multipronged therapeutic approach. In the current scenario, the use of polyherbals is increasing among the diabetics. MD-1, a poly herbal formulation is constituted as a mixture of six popular anti diabetic herbs, used in the management of Diabetes mellitus (DM). The physicochemical, biochemical and in vitro efficacy studies have been carried out to ascertain the possible mechanisms underlying the anti-diabetic action of MD-1.MethodsMD-1 was evaluated for residual toxins as per Ayurvedic Pharmacoepia of India (API) procedures. The hydro alcoholic extract of the formulation (HAEF) was evaluated for anti oxidant activity against 2, 2-diphenyl-1-picrylhydrazil (DPPH) and nitric oxide radicals in vitro. The effect of HAEF on carbohydrate digestive enzymes α-glucosidase and α-amylase was studied using biochemical assays. HAEF was studied for its glucose lowering potential in L6 myotubes and 3T3L1 preadipocytes, using 2-deoxy-D-[1-3H] glucose (2-DG) uptake assay. Effect of MD-1 on adipogenesis was evaluated in 3T3L1 adipocytes using oil O red staining. The effect of HAEF on mRNA expression of peroxisome proliferator activated receptor gamma (PPARγ) and glucose transporter 4 (GLUT4) in 3T3L1 adiocytes was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). Statistical analysis was performed by student t-test, ANOVA.ResultsResidual toxins present within the API limits and HAEF demonstrated strong antioxidant potential and significantly inhibited the α-glucosidase (IC50 63.6 ± 0.46 μg/mL) and α-amylase (IC50 242.81 ± 1.26 μg/mL) activity. HAEF significantly (p < 0.05) enhanced the insulin stimulated glucose uptake in both the cell lines studied. Unlike standard pioglitazone (PGZ), HAEF modulated the mRNA expression of PPARγ and GLUT4 (p < 0.0001) in 3T3L1 adipocytes, without inducing adipogenesis.ConclusionPhysicochemical parameters established in the study may serve as reference standards in regular quality control. Absence of residual toxins underpins the safety. The enhanced glucose uptake and favorable modulation of insulin sensitivity through a plausible weak PPARγ agonism is similar to the distinct PPARγ activation pattern of several reported natural compound agonists. The differential binding modes of such dynamic combinatorial ligands within the formulation unlike synthetic ligands like thiozolidinediones (TZD) can be linked to the safe mitigation of diabetic complications by MD-1.

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Section: Introductionmentioning

confidence: 99%

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Telapolu

Kalachavedu

Punnoose³

et al. 2018

BMC Complement Altern Med

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“…In the Ayurvedic Pharmacopoeia of India, T. cordifolia is categorized as an antidiabetic herbal drug due to its alkaloids, diterpenoids and glycosidic constituents [27]. Several studies in vitro and in vivo showed that the alkaloids palmatine, jatrorrhizine and magnoflorin were synergistically responsible for the hyperglycemic effect via a mechanism of insulin releasing, insulin-mimicking and gluconeogenesis inhibition [143][144][145]. Magnoflorine was the most potent -glucosidase inhibitor using sucrase and maltose as substrate with inhibitory values of 9.8 and 7.6 µg/mL, respectively, possibly due to blockade of glucose cotransporters Glut-2 and Glut-4, the main carriers of glucose from the intestine into circulation [144].…”

Section: Hypoglycemic Activitymentioning

confidence: 99%

“…Several studies in vitro and in vivo showed that the alkaloids palmatine, jatrorrhizine and magnoflorin were synergistically responsible for the hyperglycemic effect via a mechanism of insulin releasing, insulin-mimicking and gluconeogenesis inhibition [143][144][145]. Magnoflorine was the most potent -glucosidase inhibitor using sucrase and maltose as substrate with inhibitory values of 9.8 and 7.6 µg/mL, respectively, possibly due to blockade of glucose cotransporters Glut-2 and Glut-4, the main carriers of glucose from the intestine into circulation [144]. The antidiabetic property of palmatine was reported in myocyte L6 cells through an insulin dependent pathway and by upregulating glucose transporter-4 (Glut-4) and PPAR (peroxisome proliferator-activated receptor) expression, with berberine and palmatine having similar antidiabetic potential [145].…”

Section: Hypoglycemic Activitymentioning

confidence: 99%

Chemistry and Pharmacology of Tinospora cordifolia

Singh

Chaudhuri

2017

Natural Product Communications

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Tinospora cordifolia (Menispermaceae) is an Ayurvedic medicinal plant distributed throughout the Indian subcontinent and China. The whole plant is used in folk and the Ayurvedic system of medicine alone and in combination with other plants. Due to its commercial importance, T. cordifolia has been of intense research interest for the last four decades with the isolation of diverse compounds such as alkaloids, sesquiterpenoids, diterpenoids, phenolics, steroids, aliphatic compounds and polysaccharides, along with the discovery of a wide spectrum of pharmacological properties like immunomodulation, anticancer, hepatoprotective and hypoglycemic. Although pharmacological activities of extracts and compounds of T. cordifolia have been studied both in vitro and in vivo, only few mechanisms of action have been explored and need further elaboration. In the present review, the pharmacological activities of compounds and different extracts of T. cordifolia are highlighted, along with those of the marketed products, showing the relevance of phytochemicals and the standardization of the marketed products for medicinal use. This compilation of the extensive literature of T. cordifolia here will be a referral point for clinical study and the development of standardized phytomedicines in healthcare.

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“…Anti-diabetic activities has been well known and documented in various studies conducted worldwide. 6,7 A wide variety of active components derived from the plant like crude extracts, alkaloids, steroids, lactones and aliphatic have been isolated from the different parts of the plant body. 8 Phyllanthus emblica (syn.…”

Section: Introductionmentioning

confidence: 99%

Comparative study to evaluate the anti-diabetic activity of commercially available extract of Tinospora cordifolia and Phyllanthus emblica in streptozocin induced diabetic rat

Pathak

Bandyopadhyay

Kumar

et al. 2016

Int J Basic Clin Pharmacol

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Antidiabetic claims of Tinospora cordifolia (Willd.) Miers: critical appraisal and role in therapy

Cited by 84 publications

References 53 publications

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

MD-1, a poly herbal formulation indicated in diabetes mellitus ameliorates glucose uptake and inhibits adipogenesis – an in vitro study

Chemistry and Pharmacology of Tinospora cordifolia

Comparative study to evaluate the anti-diabetic activity of commercially available extract of Tinospora cordifolia and Phyllanthus emblica in streptozocin induced diabetic rat

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