Antidiabetic and hypolipidemic activities of eburicoic acid, a triterpenoid compound from <i>Antrodia camphorata</i>, by regulation of Akt phosphorylation, gluconeogenesis, and PPARα in streptozotocin-induced diabetic mice

Lin, Cheng-Wei; Kuo, Yueh‐Hsiung; Shih, Chun Chuan

doi:10.1039/c8ra01841c

Cited by 11 publications

(23 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antcin K inhibits the fatty acid synthase, increases peroxisome α proliferator-activated receptor (PPAR-α) expression, and reduces the mRNA expression of sterol 1c regulatory element binding proteins (SREBP-1c) in the liver, thereby contributing to the decreased TAG levels and hepatic steatosis [57]. Similar results were obtained in the evaluation of eburicoic- [58] and dehydroeburicoic acids [59], two PTs obtained from Antrodia camphorata. There are various mechanisms potentially associated with TAG levels decreasing after SA exposition; the inhibition of human carboxylesterase type 1 (hCE1), an enzyme responsible of bulky alkyl chain ester such as TAGs, clearly related with obesity and T2DM progression [50].…”

Section: Biological Activity Assessmentsupporting

confidence: 57%

Section: Biological Activity Assessmentsupporting

confidence: 57%

See 1 more Smart Citation

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

et al. 2020

View full text Add to dashboard Cite

Plant extracts from Cecropia genus have been used by Latin-American traditional medicine to treat metabolic disorders and diabetes. Previous reports have shown that roots of Cecropia telenitida that contains serjanic acid as one of the most prominent and representative pentacyclic triterpenes. The study aimed to isolate serjanic acid and evaluate its effect in a prediabetic murine model by oral administration. A semi-pilot scale extraction was established and serjanic acid purification was followed using direct MALDI-TOF analysis. A diet induced obesity mouse model was used to determine the impact of serjanic acid over selected immunometabolic markers. Mice treated with serjanic acid showed decreased levels of cholesterol and triacylglycerols, increased blood insulin levels, decreased fasting blood glucose and improved glucose tolerance, and insulin sensitivity. At transcriptional level, the reduction of inflammation markers related to adipocyte differentiation is reported.

show abstract

Section: Biological Activity Assessmentsupporting

confidence: 57%

Section: Biological Activity Assessmentsupporting

confidence: 57%

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Recently, compounds 1 and 2 were isolated from ACFB [ 30 , 37 , 38 , 39 ] and investigated for its reducing effects on blood glucose, total cholesterol, triglyceride and leptin levels in mice fed a high-fat diet (HFD) [ 30 ], as well as its antiproliferative activity [ 33 , 37 ] and inhibition against DEN-enhanced hepatocellular inflammation, fibrosis and carcinoma [ 38 ]. Compounds 3 , 7 and 8 were also obtained from ACFB [ 31 , 38 , 39 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…Compounds 1 , 2 [ 30 ], 7 [ 28 ], and 8 [ 31 ] also showed hyperglycemic and antidiabetic properties via the glucose transporter 4 (GLUT4) and palmitate-treated C2C12 myotubes in mice fed a high-fat diet. Recently, compound 8 was investigated for its anti-type 1 diabetes and hypolipidemic activities [ 40 ]. However, potent aGI activity related to type 2 diabetes or obesity treatments had not been reported in the literature for all six active compounds.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea—An Edible Taiwanese Mushroom

et al. 2018

View full text Add to dashboard Cite

Antrodia cinnamomea (AC), an edible Taiwanese mushroom, has been recognized as a valuable natural resource with vast biological and medicinal benefits. Recently, the hypoglycemic and anti-diabetic effects of AC were mentioned in several studies. However, no studies have investigated α-glucosidase inhibitors from AC fruiting bodies (ACFB) as they relate to type 2 diabetes (T2D) treatment. The purpose of this study was to gain evidence of potent α-glucosidase inhibitory effects, as well as isolate, identify and characterize the active compounds of ACFB. The MeOH extract of ACFB demonstrated potent α-glucosidase inhibitory activity, and possessed high pH stability (pH 2–11) and thermostable properties at 40–50 °C. Further purification led to the isolation of eight constituents from ACFB, identified as: 25S-antcin K (1), 25R-antcin K (2), dehydrosulphurenic acid (3), 25S-antcin I (4), 25S-antcin B (5), 25R-antcin B (6), dehydroeburicoic acid (7) and eburicoic acid (8). Notably, the ACFB extract and its identified compounds, except 1, 4, and 6 demonstrated a greater effect (EC50 = 0.025–0.21 mg/mL) than acarbose (EC50 = 0.278 mg/mL). As such, these active compounds were determined to be new potent mushroom α-glucosidase inhibitors. These active compounds were also identified on the HPLC fingerprints of ACFB.

show abstract

“…Evidence has shown that the solid culture of the fruiting body and the filtrate in submerged culture have a hepatoprotective effect and antioxidant activity [4,5]. Our recent studies demonstrated that ergostatrien-3β-ol (EK100) [6], dehydroeburicoic acid (TR2), euricoic acid (TR1) [7,8,9,10], and antcin K [11] from A. cinnamomea exhibited antihyperglycemic and antihyperlipidemic activity. Nevertheless, the antidiabetic and antihyperlipidemic potential activities of sulphurenic acid (24-methylenelanosta-8-ene-3β,15α-diol-21-oic acid, 10; TR3; SA) (Figure 1) from A. camphorata remain unknown in streptozotocin (STZ)-induced diabetic mice.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and Antihyperlipidemic Effects of Sulphurenic Acid, a Triterpenoid Compound from Antrodia camphorata, in Streptozotocin-Induced Diabetic Mice

Lin

Hsiao

Kuo

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

The present study was designed to evaluate the protective effect of sulphurenic acid (SA), a pure compound from Antrodia camphorata, on diabetes and hyperlipidemia in an animal model study and to clarify the underlying molecular mechanism. Diabetes was induced by daily 55 mg/kg intraperitoneal injections of streptozotocin (STZ) solution over five days. Diabetic mice were randomly divided into six groups and orally gavaged with SA (at three dosages) or glibenclamide (Glib), fenofibrate (Feno) or vehicle for 3 weeks. Our findings showed that STZ-induced diabetic mice had significantly increased fasting blood glucose, glycated hemoglobin (HbA1C), plasma triglyceride (TG), and total cholesterol (TC) levels (p < 0.001, p < 0.001, p < 0.001, and p < 0.05, respectively) but decreased blood insulin, adiponectin, and leptin levels compared to those of the control group (p < 0.001, p < 0.001, and p < 0.001, respectively). Administration of SA to STZ-induced diabetic mice may lower blood glucose but it increased the insulin levels with restoration of the size of the islets of Langerhans cells, implying that SA protected against STZ-induced diabetic states within the pancreas. At the molecular level, SA treatment exerts an increase in skeletal muscle expression levels of membrane glucose transporter 4 (GLUT4) and phospho-Akt to increase the membrane glucose uptake, but the mRNA levels of PEPCK and G6Pase are decreased to inhibit hepatic glucose production, thus leading to its hypoglycemic effect. Moreover, SA may cause hypolipidemic effects not only by enhancing hepatic expression levels of peroxisome proliferator-activated receptor α (PPARα) with increased fatty acid oxidation but also by reducing lipogenic fatty acid synthase (FAS) as well as reducing mRNA levels of sterol regulatory element binding protein (SREBP)1C and SREBP2 to lower blood TG and TC levels. Our findings demonstrated that SA displayed a protective effect against type 1 diabetes and a hyperlipidemic state in STZ-induced diabetic mice.

show abstract

Antidiabetic and hypolipidemic activities of eburicoic acid, a triterpenoid compound from Antrodia camphorata, by regulation of Akt phosphorylation, gluconeogenesis, and PPARα in streptozotocin-induced diabetic mice

Abstract: The study is designed to examine the potential effects and underlying mechanisms of eburicoic acid (TRR), a compound from Antrodia camphorata, in streptozotocin (STZ)-induced diabetic mice.

Cited by 11 publications

References 58 publications

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

Serjanic Acid Improves Immunometabolic Markers in a Diet-Induced Obesity Mouse Model

Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea—An Edible Taiwanese Mushroom

Antidiabetic and Antihyperlipidemic Effects of Sulphurenic Acid, a Triterpenoid Compound from Antrodia camphorata, in Streptozotocin-Induced Diabetic Mice

Contact Info

Product

Resources

About