Antidiabetic activity of medium-polar extract from the leaves of Stevia rebaudiana Bert. (Bertoni) on alloxan-induced diabetic rats

Abstract

Objective:To investigate the medicative effects of medium-polar (benzene:acetone, 1:1, v/v) extract of leaves from Stevia rebaudiana (family Asteraceae) on alloxan-induced diabetic rats.Materials and Methods:Diabetes was induced in adult albino Wistar rats by intraperitoneal (i.p.) injection of alloxan (180 mg/kg). Medium-polar extract was administered orally at daily dose of 200 and 400 mg/kg body wt. basis for 10 days. The control group received normal saline (0.9%) for the same duration. Glibenclamide was used as positive control reference drug against Stevia extract.Results:Medium-polar leaf extract of S. rebaudiana (200 and 400 mg/kg) produced a delayed but significant (P < 0.01) decrease in the blood glucose level, without producing condition of hypoglycemia after treatment, together with lesser loss in the body weight as compared with standard positive control drug glibenclamide.Conclusions:Treatment of diabetes with sulfonylurea drugs (glibenclamide) causes hypoglycemia followed by greater reduction in body weight, which are the most worrisome effects of these drugs. Stevia extract was found to antagonize the necrotic action of alloxan and thus had a re-vitalizing effect on β-cells of pancreas.

Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations

“…Malaysia does not have the optimum climate to enhance sugar cane cultivation; hence the local demand for sugar is highly dependent on sugar imports. Stevia plant is in high demand because of its potential as a non-calorific natural sweetener specifically towards individuals with body weight issues as well as diabetics (Mehta et al, 2011;Mogra & Dashora, 2009).…”

Attenuation of metabolic dysfunctions in the skeletal muscles of type 1 diabetic rats by Stevia rebaudiana extracts, via AMPK upregulation and antioxidant activities [pdf]

“…These findings suggest that CGA, stevioside and stevia extracts have anti-diabetic effects, in line with previous studies that have reported hypoglycemic effects for stevia extracts and derivatives. Previous studies have reported that stevia extracts have hypoglycemic effects in experimental animal models of DM (Assaei et al, 2016;Das et al, 2000;Misra et al, 2011). Also, Karthikesan et al (2010) demonstrated that CGA at a dose of 5 mg/kg b.w.…”

Stevia rebaudianaBertoni. Un potencial adyuvante en el tratamiento de la diabetes mellitus

“…Considerando lo anterior, algunos estudios han reportado que el extracto de S. rebaudiana y el esteviósido tienen efecto antihiperglucemiante, tanto en modelos animales (Jeppesen et al, 2002;Kujur et al, 2010;Lailerd et al, 2004;Misra et al, 2011; como en humanos (Curi et al, 1986;Gregersen et al, 2004), así como también efecto hipoglucemiante en ratas (Raskovic et al, 2008); por otra parte, el Rebaudiósido A, tanto en modelos animales como en humanos ha demostrado no tener efecto sobre la glucosa sanguínea.…”