Antidiabetic activity of<i>Kalanchoe pinnata</i>in streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action

Patil, Swapnil; Dongare, Vandana; Kulkarni, Chaitanya R.; Joglekar, Madhav M.; Arvindekar, Akalpita U.

doi:10.3109/13880209.2013.794364

Cited by 30 publications

(17 citation statements)

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“…It was reported that Korean red ginseng had a beneficial effect on the treatment of diabetes by stimulating insulin secretion by its own [ 36 ]. In addition, a phenyl alkyl ether derivative isolated from the Kalanchoe pinnata leaves showed insulin secretagogue action in glucose independent manner, which could be useful in treatment of diabetes [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells

Park

Han

2018

Nutr Res Pract

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BACKGROUND/OBJECTIVEThis study was designed to investigate how a Portulaca oleracea L. extract (POE) stimulates insulin secretion in INS-1 pancreatic β-cells.MATERIALS/METHODINS-1 pancreatic β-cells were incubated in the presence of various glucose concentrations: 1.1 or 5.6, 16.7 mM glucose. The cells were treated with insulin secretagogues or insulin secretion inhibitor for insulin secretion assay using an insulin ELISA kit. In order to quantify intracellular influx of Ca2+ caused by POE treatment, the effect of POE on intracellular Ca2+ in INS-1 pancreatic β-cells was examined using Fluo-2 AM dye.RESULTSPOE at 10 to 200 µg/mL significantly increased insulin secretion dose-dependently as compared to the control. Experiments at three glucose concentrations (1.1, 5.6, and 16.7 mM) confirmed that POE significantly stimulated insulin secretion on its own as well as in a glucose-dependent manner. POE also exerted synergistic effects on insulin secretion with secretagogues, such as L-alanine, 3-isobutyl-1-methylxanthine, and especially tolbutamide, and at a depolarizing concentration of KCl. The insulin secretion caused by POE was significantly attenuated by treatment with diazoxide, an opener of the K+ATP channel (blocking insulin secretion) and by verapamil (a Ca2+ channel blocker). The insulinotropic effect of POE was not observed under Ca2+-free conditions in INS-1 pancreatic β-cells. When the cells were preincubated with a Ca2+ fluorescent dye, Fluo-2 (acetoxymethyl ester), the cells treated with POE showed changes in fluorescence in red, green, and blue tones, indicating a significant increase in intracellular Ca2+, which closely correlated with increases in the levels of insulin secretion.CONCLUSIONSThese findings indicate that POE stimulates insulin secretion via a K+ATP channel-dependent pathway in INS-1 pancreatic β-cells.

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Section: Discussionmentioning

confidence: 99%

A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells

Park

Han

2018

Nutr Res Pract

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“…The extract has shown smooth muscle relaxing properties [ 18 , 19 ]. Other extracts from the Bryophyllum family than Kalanchoe pinnata have shown anti-diabetic activity [ 20 ] and anti-inflammatory effects [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral use

Gründemann

Diegel

Sauer

et al. 2015

BMC Complement Altern Med

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BackgroundPreparations from anthroposophical medicine (AM) are clinically used to treat inflammatory disorders. We wanted to investigate effects of a selection of AM medications for parenteral use in cell-based systems in vitro.MethodsColchicum officinale tuber D3, Mandragora D3, Rosmarinus officinale 5 % and Bryophyllum 5 % were selected for the experiments. Induction of apoptosis and necrosis (human lymphocytes and dendritic cells [DCs]) and proliferation of lymphocytes as well as maturation (expression of CD14, CD83 and CD86) and cytokine secretion (IL-10, IL12p70) of DCs were analyzed. Furthermore, proliferation of allogeneic human T lymphocytes was investigated in vitro in coculture experiments using mature DCs in comparison to controls.ResultsThe respective preparations did not induce apoptosis or necrosis in lymphocytes or DCs. Lymphocyte proliferation was dose-dependently reduced by Colchicum officinale tuber D3 while the viability was unchanged. Rosmarinus officinale 5 %, but not the other preparations, dose-dependently inhibited the maturation of immature DCs, reduced secretion of IL-10 and IL-12p70 and slightly inhibited proliferation of allogeneic CD4+ T-lymphocytes in coculture experiments with DCs.ConclusionThe selected preparations from AM for parenteral use are nontoxic to lymphocytes and DCs. Rosmarinus officinale 5 % has immunosuppressive properties on key functions of the immune system which propose further investigation.

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“…The plant I. gracilis may, therefore, behave in a similar manner as plants which possess insulin secretagogue compounds such as Kalanchoe pinnata Lam. (Crassulaceae), Momordica charantia L. (Cucurbitaceae), and Trigonella foenum graecum L. (Fabaceae) (Patil et al, 2013;Shah et al, 2006;Yibchok-Anun et al, 2006). The presence of the antioxidants flavonoids and other polyphenols in the 80% methanolic extract of I. gracilis was observed and is probably the reason why the free radical DPPH was effectively scavenged.…”

Section: Discussionmentioning

confidence: 98%

Antidiabetic, antioxidant, and TNF-α lowering properties of extract of the traditionally used plantIxeris gracilisin alloxan-induced diabetic mice

Syiem

Warjri

2014

Pharmaceutical Biology

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Antidiabetic activity ofKalanchoe pinnatain streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action

Abstract: The DCM fraction of Kalanchoe pinnata demonstrates excellent insulin secretagogue action and can be useful in treatment of diabetes mellitus.

Cited by 30 publications

References 25 publications

A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells

A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells

Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral use

Antidiabetic, antioxidant, and TNF-α lowering properties of extract of the traditionally used plantIxeris gracilisin alloxan-induced diabetic mice

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Antidiabetic activity ofKalanchoe pinnatain streptozotocin-induced diabetic rats by glucose independent insulin secretagogue action

Abstract: The DCM fraction of Kalanchoe pinnata demonstrates excellent insulin secretagogue action and can be useful in treatment of diabetes mellitus.

Cited by 30 publications

References 25 publications

A Portulaca oleracea L. extract promotes insulin secretion via a K+ATP channel dependent pathway in INS-1 pancreatic β-cells

A Portulaca oleracea L. extract promotes insulin secretion via a K+ATP channel dependent pathway in INS-1 pancreatic β-cells

Immunomodulatory effects of preparations from Anthroposophical Medicine for parenteral use

Antidiabetic, antioxidant, and TNF-α lowering properties of extract of the traditionally used plantIxeris gracilisin alloxan-induced diabetic mice

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Product

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A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells

A Portulaca oleracea L. extract promotes insulin secretion via a K⁺_ATP channel dependent pathway in INS-1 pancreatic β-cells