Antidiabetic activities of glycoprotein from pea (<i>Pisum sativum</i> L.) in STZ-induced diabetic mice

Liu, Junping; Qian, Yan-Fang; Qin, Gaoyixin; Zhao, Liyan; Chen, Guitang

doi:10.1039/d1fo00535a

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…The treatment of PPH was also found to regulate the insulin signaling in our study, which is consistent with a previous study reporting that the intragastric administration of PPH upregulated the gene expressions of IRS1 and IRS2 in the liver of diabetic mice, and reduced insulin resistance [ 16 ]. In addition, the ingestion of PPH enhanced the postprandial insulin secretion in the healthy subjects [ 29 ].…”

Section: Discussionsupporting

confidence: 93%

“…The PPH sample was subjected to the use of a nano-UPLC (EASY-nLC1200) coupled with the use of a Q Exactive HFX Orbitrap instrument (Thermo Fisher Scientific, Waltham, MA, USA) with a reversed-phase column (100 μm ID × 15 cm, Reprosil-Pur 120 C18-AQ, 1.9 μm) for separation based on a previous study, with modifications [ 16 ]. Acetonitrile (2% ACN) with 0.1% formic acid (FA) in H 2 O and 80% ACN with 0.1% FA in H 2 O were used as mobile phase A and phase B, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Liao¹,

Cao²,

Xia³

et al. 2022

IJERPH

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Pea protein is considered to be a high quality dietary protein source, but also it is an ideal raw material for the production of bioactive peptides. Although the hypoglycemic effect of pea protein hydrolysate (PPH) has been previously reported, the underlying mechanisms, in particular its effect on the hepatic gluconeogenesis, remain to be elucidated. In the present study, we found that PPH suppressed glucose production in mouse liver cell-line AML-12 cells. Although both of the gluconeogenic and insulin signaling pathways in the AML-12 cells could be regulated by PPH, the suppression of glucose production was dependent on the inhibition of the cAMP response element-binding protein (CREB)-mediated signaling in the gluconeogenic pathway, but not the activation of insulin signaling. Findings from the present study have unveiled a novel role of PPH underlying its anti-diabetic activity, which could be helpful to accelerate the development of functional foods and nutraceuticals using PPH as a starting material.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Liao¹,

Cao²,

Xia³

et al. 2022

IJERPH

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“…It is much more toxic than oxygen-derived free radicals, requiring CAT and GPx to remove this. It has been found that BSSCE can promote glucose metabolism by regulating glycogen synthesis in diabetic mice, and SOD, CAT, and GPx activity in mouse livers was also greatly improved [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Wen

et al. 2022

Nutrients

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Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidative stress indicators, such as ROS, GSH/GSSG, MDA, SOD, CAT, GPx, and GR, were tested with biochemical kit. Protein expression of Keap1, Nrf2, and TXNIP was measured by Western blot. Compared with the control group, Pb reduced the cell viability of PC12 cells. However, BSP treatment significantly increased the viability of PC12 cells induced by lead exposure (p < 0.05). Lead can enrich the contents of MDA and ROS, but decrease the amount of CAT, SOD, GR, GPx, and GSH/GSSG in PC12 cells, while BSP can alleviate it (p < 0.05). Lead can enhance the expression of Keap1 and TXNIP proteins, but reduce Nrf2 expression. In contrast, BSPs reversed this phenomenon (p < 0.05). BSPs can alleviate oxidative stress injury induced by lead in PC12 cells through the Keap1/Nrf2/TXNIP signaling pathway.

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“…Here we found no between-group differences in HOMA-IR except a trend towards a reduction in insulin resistance in the PEA group compared to the CAS group. Recent studies have shown that pea glycoproteins and peptides have antidiabetic activities, in particular by reducing insulin resistance [48,49]. Therefore, it may be possible that long-term pea protein consumption could improve age-related insulin resistance in old rats.…”

Section: Metabolic Parametersmentioning

confidence: 99%

Pea Proteins Have Anabolic Effects Comparable to Milk Proteins on Whole Body Protein Retention and Muscle Protein Metabolism in Old Rats

et al. 2021

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Plant proteins are attracting rising interest due to their pro-health benefits and environmental sustainability. However, little is known about the nutritional value of pea proteins when consumed by older people. Herein, we evaluated the digestibility and nutritional efficiency of pea proteins compared to casein and whey proteins in old rats. Thirty 20-month-old male Wistar rats were assigned to an isoproteic and isocaloric diet containing either casein (CAS), soluble milk protein (WHEY) or Pisane™ pea protein isolate for 16 weeks. The three proteins had a similar effect on nitrogen balance, true digestibility and net protein utilization in old rats, which means that different protein sources did not alter body composition, tissue weight, skeletal muscle protein synthesis or degradation. Muscle mitochondrial activity, inflammation status and insulin resistance were similar between the three groups. In conclusion, old rats used pea protein with the same efficiency as casein or whey proteins, due to its high digestibility and amino acid composition. Using these plant-based proteins could help older people diversify their protein sources and more easily achieve nutritional intake recommendations.

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Antidiabetic activities of glycoprotein from pea (Pisum sativum L.) in STZ-induced diabetic mice

Abstract: Glycoprotein PGP2 had good hypoglycemic activity and was expected to be developed as a natural medicine for the treatment of type II diabetes.

Cited by 15 publications

References 24 publications

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Pea Protein-Derived Peptides Inhibit Hepatic Glucose Production via the Gluconeogenic Signaling in the AML-12 Cells

Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Pea Proteins Have Anabolic Effects Comparable to Milk Proteins on Whole Body Protein Retention and Muscle Protein Metabolism in Old Rats

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