Antidepressant-like activity of L-701324 in mice: A behavioral and neurobiological characterization

Antidepressant-like activity of oroxylin A in mice models of depression: A behavioral and neurobiological characterization

“…SLAC Laboratory Animal Co., Ltd. (Shanghai, China) provided C57BL/6J mice (male, 8–10 weeks) for this study. Before use, animals were housed (4 per cage, M2 type) under standard conditions (12 h of light/dark cycle, lights on from 07:00 to 19:00 h; (23 ± 1)°C ambient temperature; (55 ± 10)% relative humidity; noise less than 50 dB; ammonia concentration less than 14 mg m −3 ; 24 h of air circulation; and bedding replacement twice a week) for 1 week with ad libitum access to water and rodent chow ( Guan et al, 2021 ; Liu et al, 2021 ; Zhao et al, 2021 ). The mice were subjected to stratified randomization according to their body weights.…”

“…Forced swim test (FST) is a most widely used behavioral assay for detecting potential antidepressants ( Petit-Demouliere et al, 2005 ). As described in several previous reports ( Guan et al, 2021 ; Liu et al, 2021 ; Zhao et al, 2021 ), mice were individually placed in a transparent glass tank (45 cm height, 20 cm internal diameter) containing fresh water (15 cm high, 25 ± 1 °C) for a 6-min period. Afterwards, each mouse was dried and returned to its home cage.…”

“…Similar to FST, Tail suspension test (TST) is also widely adopted to screen potential antidepressants ( Cryan et al, 2005 ). As described in several previous reports ( Guan et al, 2021 ; Liu et al, 2021 ; Zhao et al, 2021 ), mice were individually glued to a rail 70 cm above the floor by adhesive taps for a 6-min period, and the immobility during the whole period was recorded by an observer unaware of animal grouping. Afterwards, each mouse was returned to its home cage.…”

“…To exclude this possibility, Open field test (OFT) was used. As described in several previous reports ( Guan et al, 2021 ; Liu et al, 2021 ; Zhao et al, 2021 ), mice were individually introduced into an open field apparatus (100 × 100 × 45 cm; 25 squares) for a 5-min period. The apparatus was illuminated with a red bulb (50 W) on the ceiling.…”

“…In addition, many substances which are able to promote BDNF biosynthesis (xanthoceraside, L-701324, 1-methylnicotinamide, etc.) have been reported to protect against depression ( Guan et al, 2021 ; Liu et al, 2021 ; Zhao et al, 2021 ). By analyzing these literatures collectively, it is possible that like xanthoceraside, L-701324 and 1-methylnicotinamide, oroxylin A also possesses antidepressant-like efficacy in rodents by enhancing the BDNF system.…”