Anticuerpos antihipófisis en pacientes con sospecha de hipofisitis autoimmune

Chimenti, Elisa Moya; Doforno, Rita Álvarez; Bajo, África Villaroel; Frutos, R.; Sánchez, Laura García; Escolá, Cristina Álvarez

doi:10.1016/j.endonu.2010.02.002

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…Moya Chimenti [ 22 ] reported that pituitary antibodies are present in 43% of patients with isolated ACTH deficiency. On the other hand, Yamamoto [ 23 ] describes the presence of antibodies against the pituitary transcription factor PIT-1 in patients with growth hormone prolactin and TSH insufficiency.…”

Section: Discussionmentioning

confidence: 99%

Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing

Gut¹,

Fischbach²,

Ziemnicka³

et al. 2014

cejoi

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IntroductionPituitary autoantibodies can be determined both in patients with pituitary disease as well as patients with autoimmune endocrine diseases. The purpose of the study was to isolate and purify pituitary autoantigen using sera of patients and the microsomal fraction of the pituitary.Material and methodsTo isolate a pituitary autoantigen, patient sera were used, which showed a strong immune response to pituitary antigens. Pituitary microsomal fractions were prepared from pituitary tissue homogenates. In the study, sera of patients with pituitary disease, Addison and Graves’ disease were used. The initial stages were carried out by affinity chromatography on CN -Br sepharose column whereas purification was continued by column liquid chromatography on AcA54 Ultrogel. Chromatofocusing was performed by Polybuffer exchanger PBE 94.Results 125I-labeled pituitary antigens after isolation appeared in column chromatography in three peaks. The first peak contained 50-70 kDa proteins, the second peak – 17 to 22 kDa proteins and the third peak contains 125-iodides. Three fractions obtained from filtration on Ultrogel were separated in a polyacrylamide gel. In the first peak two bands 67 and 55 kDa appeared. The second peak contained low molecular weight substances, and the third peak contained 125I. The first peak from Ultrogel was isolated by chromatofocusing – the first peak with pH 5.9 and the second one with pH 4.9.ConclusionsIsolation and purification of pituitary autoantigen with the use of column liquid chromatography and chromatofocusing resulted in obtainment of two antigenic proteins of specific gravity of 67 and 55 kDa.

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Section: Discussionmentioning

confidence: 99%

Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing

Gut¹,

Fischbach²,

Ziemnicka³

et al. 2014

cejoi

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“…Serum APA have been measured so far in pituitary adenoma patients using different techniques (mainly immunofluorescence, immunoblotting, ELISA, and radioligand assay) and were found to have a prevalence ranging between 0 and 30% [6,36,37]. Even more, a high prevalence of APA-positivity has been detected in patients with autoimmune thyroid disease (AITD), particularly those with Hashimoto's thyroiditis.…”

Section: Pituitary Tumors and Immune System: Marriage Inexploredmentioning

confidence: 99%

Immune System and Pituitary Tumors: TILs Death tears them Apart. A Review

Leon¹

2015

J Cytol Histol

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Introduction: Pituitary tumors are common in general population, but the study of these tumors confronts some restrictions, including the inaccessibility of the gland for biopsy material. It has also been described that lymphocytic infiltration can be found adjacent to pituitary adenomas. Objective: This article was conceived to collect some information to describe the involvement of immune system on pituitary tumors microenvironment and also to shed light to molecular mechanisms implicated on the pathogenesis of these diseases. Methods: A search of the literature was done in Medline database between 1990 and 2014, using the search terms immune system and pituitary tumors. A review of 88 papers was performed including articles published mainly in English-language and with free access to full paper. The most recent references were used whenever possible. Results: Cell-mediated immunity appears to be predictive of a less favorable clinical outcome. Also sera antipituitary antibodies can be obtained from patients with those tumors. Low sensitivity of individual autoantibodies makes them, at present, a poor diagnostic marker for clinical purposes. Conclusions: Nonetheless, tumors infiltrating lymphocytes (TILs) and anti-pituitary antibodies (APA) still make part of a promising calling for diagnosis and treatment of pituitary tumors; new approaches to further understanding of immune response enrollment on pituitary tumor microenvironment are still needed.

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