Anticonvulsant effects of the wasp Polybia ignobilis venom on chemically induced seizures and action on GABA and glutamate receptors

Cunha, Alexandra Olímpio Siqueira; Mortari, Márcia Renata; Oliveira, Luciana Coutinho de; Carolino, Ruither Oliveira Gomes; Coutinho‐Netto, Joaquim; Santos, Wagner Ferreira dos

doi:10.1016/j.cca.2005.05.004

Cited by 25 publications

(22 citation statements)

References 40 publications

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“…The SrTx1.3 toxin isolated from this venom was also capable of protecting rats against seizures induced by the administration of bicuculline in the area tempestas when administrated in the SNpr (Mussi- Ribeiro et al 2004). Likewise, Cunha et al (2005) showed that lower doses of the crude venom of the wasp Polybia ignobilis Haliday via intracerebroventricular (i.c.v.) provoked severe generalized tonic-clonic seizures.…”

Section: Introductionmentioning

confidence: 96%

“…Because very few studies have focused on the venoms of ants as sources of excitatory and inhibitory neuronal modulators, the aim of this study was to investigate the effects of the intracerebroventricular injection of the D. quadriceps ant venom on mice behavior in the open field. Considering the fact that the crude venom from invertebrates sometimes induces seizures when tested in rodents whereas the denatured venom causes the opposite effect (Cairrão et al 2002, Cunha et al 2005, we also evaluated the anticonvulsant potential of the denatured venom on seizures induced by the administration of bicuculline in mice.…”

Section: Introductionmentioning

confidence: 99%

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Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

et al. 2015

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Epilepsy affects at least 50 million people worldwide, and the available treatment is associated with various side effects. Approximately 20-30% of the patients develop seizures that persist despite careful monitored treatment with antiepileptic drugs. Thus, there is a clear need for the development of new antiepileptic drugs, and the venoms can be an excellent source of probes. In this context, while there are studies on venoms from snakes, scorpions, and spiders, little is known regarding venom from ants. The aim of this study was to investigate the potential pro- and anticonvulsant effects of the venom from the ant Dinoponera quadriceps (Kempf) in Swiss mice. After the injection of the crude venom (DqTx-5, 50, and 500 mg/mL) in the lateral ventricle of mice, we observed a reduction of exploration and grooming behaviors, as well as an increase in immobility duration. In addition, the crude venom induced procursive behavior and tonic-clonic seizures at the highest concentration. Conversely, the preadministration of the denatured venom (AbDq) at the concentration of 2 mg/mL protected the animals against tonic-clonic seizures (66.7%) and death (100%) induced by administration of bicuculline. Taken together, the findings demonstrate that D. quadriceps venom might be potential source of new pro- and anticonvulsants molecules.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

et al. 2015

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“…injection of bicuculline, picrotoxin, and kainic acid, but it was ineffective against seizures caused by systemic pentylenetetrazole. Moreover, the 3 H-glu binding in membranes from rat brain cortex was inhibited by the denatured venom in lower concentrations than the 3 H-GABA binding [40]. The denatured venom of the Polybia occidentalis wasp inhibited convulsing action of bicuculline (ED 50 57 g/L), picrotoxin (ED 50 75 g/L), and kainic acid (ED 50 44 g/L), although it was ineffective against PTZ-induced seizures [41].…”

Section: Discussionmentioning

confidence: 99%

Effects of the paratemnus elongatus pseudoscorpion venom in the uptake and binding of the L‐glutamate and GABA from rat cerebral cortex

Santos¹,

Coutinho‐Netto²

2006

J Biochem & Molecular Tox

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L-Glu is the most important and widespread excitatory neurotransmitter of the vertebrates. Four types of receptors for L-glu have been described. This neurotransmitter modulates several neuronal processes, and its dysfunction causes chronic and acute diseases. L-Glu action is terminated by five distinct transporters. Antagonists for these receptors and modulators of these transporters have anticonvulsant and neuroprotective potentials, as observed with the acylpoliamines and peptides isolated from spiders, solitary and social wasp venoms. On the other hand, the major inhibitory neurotransmitter in mammalian nervous tissue is the GABA. Drugs that enhance GABA neurotransmission comprise effective approaches to protecting the brain against neuronal injury. Is this study, we demonstrate for the first time the inhibition of the [3H]L-glu binding to its specific sites in synaptosomal membranes from rat cerebral cortex, produced by 0.027 U of Paratemnus elongatus venom (EC50). The venom of P. elongatus changes Km and Vmax into the high affinity uptake of the L-glu and decreases Km and Vmax into the parameters of the GABA uptake from rat synaptosomes. This leads us to speculate on the possible presence of selective and specific compounds in this venom that act in L-glu and GABA dynamics, and therefore, that can serve as tools and new drug models for understanding these neurotransmissions.

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“…[23242526] The venom of social wasps contains mainly low-molecular-weight compounds comprising peptides,[2728] which are the most common compounds (about 70%) in this venom[28] and many of these have antimicrobial, myotoxic, hemolytic, and neural activity. [26293031] In relation to the latter, some peptides exert potent anticonvulsant, antinociceptive, and antipanic activity when applied directly to the central nervous system of rats. [303133]…”

Section: Introductionmentioning

confidence: 99%

“…[26293031] In relation to the latter, some peptides exert potent anticonvulsant, antinociceptive, and antipanic activity when applied directly to the central nervous system of rats. [303133]…”

Section: Introductionmentioning

confidence: 99%

Anticonvulsant and anxiolytic activity of the peptide fraction isolated from the venom of the social wasp Polybia paulista

Couto¹,

Anjos²,

Araujo³

et al. 2012

Phcog Mag

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Background:Arthropod venoms have attracted interest because they represent a source of neuroactive compounds that can be useful tools in neuroscience and pharmacological investigations.Objective:The purpose of the present work was to evaluate the anticonvulsant, anxiolytic, and behavioral effects of the peptide fraction separated from venom of the social wasp.Materials and Methods:The low- molecular-weight compounds of the venom were separated by ultrafiltration and the bioassays were performed to test anticonvulsant and anxiolytic effects, as well as alterations in the spontaneous behavior of the animals.Results:Intracerebroventricular injections of the compounds induced dose-dependent anticonvulsant effects and a potent anxiolytic activity. Regarding behavioral effects, no significant differences were observed in relation to the saline control group.Conclusion:The low-molecular-weight compounds of the venom of Polybia paulista include neuroactive peptides that can be used as pharmacological resources for anticonvulsant and anxiolytic drug research.

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Anticonvulsant effects of the wasp Polybia ignobilis venom on chemically induced seizures and action on GABA and glutamate receptors

Cited by 25 publications

References 40 publications

Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

Effects of the paratemnus elongatus pseudoscorpion venom in the uptake and binding of the L‐glutamate and GABA from rat cerebral cortex

Anticonvulsant and anxiolytic activity of the peptide fraction isolated from the venom of the social wasp Polybia paulista

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