Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

Nôga, Diana Aline; Cagni, Fernanda Carvalho; Santos, José R.; Silva, D; Azevedo, Dina Lillia Oliveira de; Araújo, Arrilton; Silva, R H; Ribeiro, Alessandra Mussi

doi:10.1007/s13744-015-0292-7

Cited by 9 publications

(6 citation statements)

References 42 publications

(60 reference statements)

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“…No effects of the venom were observed in animals that received pilocarpine or strychnine. These results indicated that D. quadriceps venom has compounds with convulsant and anticonvulsant properties, which was corroborated in a previous study from our group [ 21 ]. The fractions used herein showed a higher increase in latency to the onset of seizures, and also promoted increases in the latency to death, in survival, and protection against tonic-clonic seizures compared to those of Lopes et al [ 20 ].…”

Section: Discussionsupporting

confidence: 91%

“…Additionally, our group observed procursive behavior and tonic-clonic seizures after injecting the crude venom into the lateral ventricle of mice. In contrast, prior administration of the denatured venom protected the animals against tonic-clonic seizures (66.7%) and death (100%) induced by the gamma aminobutyric acid GABAA antagonist bicuculline (BIC) [ 21 ]. Taken together, these findings demonstrate that D. quadriceps venom is a potential source of new pro- and anticonvulsants molecules.…”

Section: Introductionmentioning

confidence: 99%

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Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Nôga

Brandão

Cagni

et al. 2016

Toxins

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Natural products, sources of new pharmacological substances, have large chemical diversity and architectural complexity. In this context, some toxins obtained from invertebrate venoms have anticonvulsant effects. Epilepsy is a neurological disorder that affects about 65 million people worldwide, and approximately 30% of cases are resistant to pharmacological treatment. Previous studies from our group show that the denatured venom of the ant Dinoponera quadriceps (Kempt) protects mice against bicuculline (BIC)-induced seizures and death. The aim of this study was to investigate the anticonvulsant activity of compounds isolated from D. quadriceps venom against seizures induced by BIC in mice. Crude venom was fractionated by high-performance liquid chromatography (HPLC) resulting in six fractions referred to as DqTx1–DqTx6. A liquid chromatography-mass spectrometry (LC/MS) analysis revealed a major 431 Da compound in fractions DqTx1 and DqTx2. Fractions DqTx3 and DqTx4 showed a compound of 2451 Da and DqTx5 revealed a 2436 Da compound. Furthermore, the DqTx6 fraction exhibited a major component with a molecular weight of 13,196 Da. Each fraction (1 mg/mL) was microinjected into the lateral ventricle of mice, and the animals were observed in an open field. We did not observe behavioral alterations when the fractions were given alone. Conversely, when the fractions were microinjected 20 min prior to the administration of BIC (21.6 nM), DqTx1, DqTx4, and DqTx6 fractions increased the latency for onset of tonic-clonic seizures. Moreover, all fractions, except DqTx5, increased latency to death. The more relevant result was obtained with the DqTx6 fraction, which protected 62.5% of the animals against tonic-clonic seizures. Furthermore, this fraction protected 100% of the animals from seizure episodes followed by death. Taken together, these findings indicate that compounds from ant venom might be a potential source of new anticonvulsants molecules.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Nôga

Brandão

Cagni

et al. 2016

Toxins

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“…BIC‐induced seizures model provokes neuronal alterations in rodents similar to the electroencephalographical (EEG) discharge on the epilepsy patients [57, 58]. Moreover, this model has been showing effectiveness in the evaluation of novel candidates to be considered available for the treatment of epilepsy [39, 59–69]. Thus, we used this model to investigate the possible anticonvulsant effect of SAK in mice.…”

Section: Discussionmentioning

confidence: 99%

“…The severity of seizures was analyzed by modified Racine scale [38] (adapted by Nôga et al [39]): (1) myoclonic jerks of contralateral paw; (2) mild paw clonus lasting at least 5 s; (3) severe paw clonus lasting at least 15 s; (4) rearing in addition to severe paw clonus; and (5) rearing and falling in addition to severe paw clonus. Latency to the onset of tonic–clonic seizures (time between outset of behavioral assessment and first episode of score‐5 seizure), latency to the death (time interval until death), and percentage of protection against tonic–clonic seizures (number of animals that develop tonic–clonic seizure/number of animals in the group × 100) and survival (number of animals that survived/number of animals in the group × 100) were also considered.…”

Section: Methodsmentioning

confidence: 99%

Sakuranetin exerts anticonvulsant effect in bicuculline‐induced seizures

Vicente-Silva

Silva-Freitas

Beserra-Filho

et al. 2022

Fundamemntal Clinical Pharma

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Epilepsy is a chronic neurological disorder characterized by an abnormal, spontaneous, and synchronized neuronal hyperactivity. Therapeutic approaches for controlling epileptic seizures are associated with pharmacoresistance and side effects burden. Previous studies reported that different natural products may have neuroprotector effects. Sakuranetin (SAK) is a flavanone with antiparasitic, anti‐inflammatory, antimutagenic, antiallergic, and antioxidant activity. In the present work, the effect of SAK on seizures in a model of status epilepticus induced by bicuculline (BIC) in mice was evaluated. Male Swiss mice received an intracerebroventricular injection (i.c.v.) of SAK (1, 10, or 20 mg/kg—SAK1, SAK10, or SAK20). Firstly, animals were evaluated in the open field (OF; 20 min), afterwards in the elevated plus maze (EPM) test (5 min). Next, 30 min prior the administration of BIC (1 mg/kg), mice received an injection of SAK (1 or 10 mg/kg, i.c.v.) and were observed in the OF (20 min) for seizures assessment. After behavioral procedures, immunohistochemical analysis of c‐Fos was performed. Our main results showed that the lowest doses of SAK (1 and 10 mg/kg) increased the total distance traveled in the OF, moreover protected against seizures and death on the BIC‐induced seizures model. Furthermore, SAK treatment reduced neuronal activity on the dentate gyrus of the BIC‐treated animals. Taken together, our results suggest an anticonvulsant effect of SAK, which could be used for the development of anticonvulsants based on natural products from herbal source.

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“…Experimental studies using the crude venom of D. quadriceps (DqV) demonstrated antinociceptive effect in mice models of chemical, mechanical and thermal nociception (Sousa et al, 2012). Other pharmacological properties were also demonstrated for DqV, such as antimicrobial (Lima et al, 2014), antiplatelet, anticoagulant (Madeira et al, 2015), pro-and anti-inflammatory (Sousa et al, 2016), and pro-and anticonvulsant (Nôga et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Venom of the giant ant Dinoponera quadriceps attenuates inflammatory pain in mouse cutaneous wound healing model

Assreuy

Adjafre

Sousa

et al. 2020

Acta Sci. Biol. Sci.

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Arthropod venoms are potential sources of bioactive substances, providing tools for the validation of popular use and new drugs design. Ants belonging to the genus Dinoponera are used in the folk medicine to treat inflammatory conditions. It was previously demonstrated that the venom of the giant ant Dinoponera quadriceps (DqV), containing a mixture of polypeptides, elicit antinociceptive effect in mice models of chemical, mechanical and thermal nociception. The aim of this study was to evaluate DqV antiinflammatory and antihypernociceptive effects in a mice model of traumatic cutaneous wound. Colonies of D. quadriceps were collected in the ''Serra de Maranguape'' (State of Ceará, northeastern Brazil), a small mountain range located on the coastal zone, and the venom secreted by the ant glands was extracted with capillary tubes, further lyophilized and maintained at -20 ± 1ºC until use. Wounds were performed in the dorsum of Swiss mice. Animals received intravenous (i.v.) injection of DqV (50 μg -1 kg day -1 ) during 3 days for evaluation of inflammatory parameters present in the wounds: hypernociception, leukocyte infiltrate, myeloperoxidase activity, nitrite nitrate -1 content. Data was tested by two-way ANOVA and Bonferroni's post-hoc test. DqV reduced (2.7 folds) hypernociception at 48 hours, leukocyte infiltration by 65% at 6 hours and myeloperoxidase activity by 60% at 0.5 hour after wound induction. In conclusion, the venom extracted from D. quadriceps glands attenuates inflammation and hypernociception in mice cutaneous wounds.

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Pro- and Anticonvulsant Effects of the Ant Dinoponera quadriceps (Kempf) Venom in Mice

Cited by 9 publications

References 42 publications

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Anticonvulsant Effects of Fractions Isolated from Dinoponera quadriceps (Kempt) Ant Venom (Formicidae: Ponerinae)

Sakuranetin exerts anticonvulsant effect in bicuculline‐induced seizures

Venom of the giant ant Dinoponera quadriceps attenuates inflammatory pain in mouse cutaneous wound healing model

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