Anticonvulsant Effect of Hydroalcoholic Seed Extract of Croton Tiglium in Rats and Mice

Mudium, Raghunandan; Kolasani, Bhanu Prakash

doi:10.7860/jcdr/2014/7022.4095

Cited by 2 publications

(3 citation statements)

References 3 publications

(3 reference statements)

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“…It also promotes the entry of other positive ions like Na + , the blockade of which can prevent MES-induced tonic extension (Venkatanarayana et al, 2013). The electrical seizures were induced by an electroconvulsiometer in which test animals were subjected to non-lethal electric shock using a set of electrodes applied to ear pinna or cornea (Mudium and Kolasani, 2014). The time elapsed from the shock deliverance to the onset of tonic limb extension was prolonged by the 70% ethanol, ethyl acetate, and n-hexane fractions of Cyperus rotundus (CR) significantly though incomparable to sodium valproate (Table I).…”

Section: Resultsmentioning

confidence: 99%

“…A preliminary screening test was conducted to include only rats that had shown HLE in this experimental model. The experimental drugs were administered one hour before the electrical shock (Mudium and Kolasani, 2014;Biradar et al, 2010). The test was considered positive if the animal exhibited tonic extensor seizure with rearward HLE, i.e., when the angle to the body exceeded 90 o , and sustained for more than 3 s following 10 s after stimulation (Castel-Branco et al, 2009).…”

Section: Maximal Electroshock (Mes)-induced Convulsion In Ratsmentioning

confidence: 99%

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Neuropharmacological Activity of Nut Grass (Cyperus Rotundus L.) Rhizome Fraction

Dwita

Dewanti²,

Ladeska³

et al. 2018

Pharmaciana

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The flavonoids content of Cyperus rotundus rhizomes is presumed to show activities on a central nervous system involving GABAergik. This research aimed to further investigate the neuropharmacological properties of Cyperus rotundus rhizomes fraction by measuring their hypnotic-sedative and anticonvulsant activities. The exploratory behavior test was performed using the hole-board method, while the motor coordination was assessed using the rotarod test. The results showed that the 70% ethanol fraction of Cyperus rotundus rhizome exhibited significant neuropharmacological activities (p < 0.05). Among the three fractions tested in this research (i.e., ethanol, ethyl acetate, and n-hexane), the ethanol fraction showed the best hypnotic-sedative and anticonvulsant activities. The ethanol fraction of Cyperus rotundus rhizome could shorten the duration of HLE equal to sodium valproate and potentiate phenobarbital-induced sleep by reducing the onset and prolonging the duration of sleep. The highest dose of the 70% ethanol fraction of Cyperus rotundus rhizome (356.7 mg/kg) displayed the best anxiolytic activity, resembling that of diazepam (p>0.05). In conclusion, Cyperus rotundus rhizome appeared as potent neuropharmacological drug candidate with abilities comparable to synthetic drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Maximal Electroshock (Mes)-induced Convulsion In Ratsmentioning

confidence: 99%

Neuropharmacological Activity of Nut Grass (Cyperus Rotundus L.) Rhizome Fraction

Dwita

Dewanti²,

Ladeska³

et al. 2018

Pharmaciana

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show abstract

“…TS is an important traditional medicine for treating constipation, dyspepsia, and dysenteria [ 8 – 11 ]. Mature Croton tiglium contains several anti-cancer components, including croton alkaloid, flavonoids, and diterpenes [ 30 – 32 ]. Croton oil from seeds of Croton tiglium is known to exert a variety of remarkable biological activities, including purgative, analgesic, antimicrobial, and inflammatory properties [ 8 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats

Yun

Kwon

Kim

et al. 2018

BMC Complement Altern Med

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BackgroundSeed of mature Croton tiglium Linne, also known as Tiglium seed (TS), has been widely used as a natural product due to its several health beneficial properties including anti-tumor and antifungal activities. Despite its ethnomedicinal beneficial properties, toxicological information regarding TS extract, especially its long-term toxicity, is currently limited. Therefore, the objective of the present study was to evaluate acute and subchronic toxicity of TS extract in rats after oral administration following test guidelines of the Organization for Economic Cooperation and Development (OECD).MethodsToxicological properties of TS extract were evaluated by toxicity assays to determine its single-dose acute toxicity (125, 250, 500, 1000, or 2000 mg/kg), 14-day repeated-dose toxicity (125, 250, 500, 1000, or 2000 mg/kg) and 13-week repeated-dose toxicity (31.25, 62.5, 125, 250, and 500 mg/kg) in Sprague-Dawley rats and F344 rats. Hematological, serum biochemical, and histopathological parameters were analyzed to determine its median lethal dose (LD50) and no-observed-adverse-effect-level (NOAEL).ResultsOral single dose up to 2000 mg/kg of TS extract resulted in no mortalities or abnormal clinical signs. In 13-week toxicity study, TS extract exhibited no dose-related changes (mortality, body weight, food/water consumption, hematology, clinical biochemistry, organ weight, or histopathology) at dose up to 500 mg/kg, the highest dosage level suggested based on 14-day repeat-dose oral toxicity study.ConclusionAcute oral LD50 of TS extract in rats was estimated to be greater than 2000 mg/kg. NOAEL of TS extract administered orally was determined to be 500 mg/kg/day in both male and female rats. Results from these acute and subchronic toxicity assessments of TS extract under Good Laboratory Practice regulations indicate that TS extract appears to be safe for human consumption.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2315-5) contains supplementary material, which is available to authorized users.

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Anticonvulsant Effect of Hydroalcoholic Seed Extract of Croton Tiglium in Rats and Mice

Cited by 2 publications

References 3 publications

Neuropharmacological Activity of Nut Grass (Cyperus Rotundus L.) Rhizome Fraction

Neuropharmacological Activity of Nut Grass (Cyperus Rotundus L.) Rhizome Fraction

Assessment of acute, 14-day, and 13-week repeated oral dose toxicity of Tiglium seed extract in rats

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