Bacterial infections are a nuisance to mankind from prehistoric times and still alive particularly in third world countries, where medicinal plants are extensively used as a treatment to pathogens since the known human history. In the current investigation, we used the crude extracts of an annual Holy Basil plant (Ocimum basilicum L. Family: Lamiaceae) enriched with a variety of aromatics, against the virulence of five pathogenic bacteria i.e., Staphylococcus aureus, Pseudomonas aeruginosa, Escheria coli, Proteus mirabilis and Klebsiella pneumoni. Among the crude ethanolic and hexane extracts, the latter showed much higher antibacterial activity than the former. Phytochemical profiling confirmed the presence of certain organic compounds (terpenoids, flavonoids, phenolics, tannins and coumarines) in the crude basil extracts, bearing strong antibacterial properties. Antibacterial chemistry was studied through thin layer chromatography (TLC) analyses with selected Pakistani varieties of basil, and it was confirmed the presence of bioactive compounds like alkaloids and terpenoids (15% sulphuric acid and Dragedroff's reagent) in hexane extracts. The study sets further need for exploration of structure of these bioactive compounds with biological effect for pharmaceutical industry and future studies.