Immunomodulatory Activity of Glycyrrhiza glabra Extract against Mixed Eimeria Infection in Chickens

Abstract

This study was conducted to evaluate the immunomodulatory activity of aqueous methanolic extract (AME) of Glycyrrhiza glabra (roots) against mixed Eimeria species infection in broiler chickens. For in vivo trial, 175 day old broiler chicks were divided into five equal groups (A, B, C, D and E). At one week of age, groups A, B and C were given orally three graded doses of G. glabra AME (100, 200 and 300 mg/kg of body weight respectively) for three consecutive days. Group D served as positive (Vitamin E treated) and Group E as negative control (PBS treated). At 14 th day of age, all groups were infected orally with 60,000 sporulated oocysts of mixed Eimeria species. Cell mediated immune response was evaluated by four tests (Phytohemagglutinin-P, Concanavalin-A, Carbon clearance assay and Dinitrochlorobenzene). Humoral immune response was evaluated by microplate hemagglutination test using sheep red blood cells. Results revealed a dose dependent immune response in G. glabra AME treated groups. Cell mediated and humoral immune response of group treated with G. glabra AME @ 300 mg/kg of body weight was almost similar (P˃0.05) to the positive control group (Vitamin E treated). G. glabra AME treated groups showed significantly higher (P<0.05) cell mediated and humoral response as compared to negative control (PBS treated).

Efficacy of a commercial herbal formula in chicken experimental coccidiosis

“…These two herbs were included in the 1st tested herbal formula. Other herbs used in H3 composition like Urtica dioica , Rosmarinus officinalis , Tanacetum vulgare , Coriandrum sativum or Glycyrrhiza glabra are known to have immunomodulatory effects [33, 41–44]. Moreover, many of these plants contain flavonoids, tannins or saponins that act as antioxidants which reduce the oxidative stress caused by reactive oxygen species encountered also in coccidiosis [27].…”

Seroprevalance of Mycoplasma gallisepticum and Mycoplasma synoviae in Commercial Broilers and Backyard Poultry in Five Districts of Khyber Pakhtunkhwa-Pakistan

“…In Pakistan poultry industry is the second largest industry with 11.8% annual growth rate (Anonymous, 2014(Anonymous, -2015. Numerous infectious diseases are responsible for economic losses to this industry (Abbas et al, 2017a(Abbas et al, , 2017b(Abbas et al, , 2017cHussain et al, 2017;Idris et al, 2017) but avian mycoplasmosis is one of the major causes due to Chronic Respiratory Disease (CRD) caused by MG and synovitis and subclinical respiratory infection caused by MS (Ehtisham-ul-haq et al, 2011, Catania et al, 2016. These mycoplasma infections result in weight loss, poor meat production, high FCR, low egg output, more embryonic mortalities and bad carcass condition (Kleven and Noel, 2008).…”

In-ovo Antiviral Effect of Nigella sativa Extract against Newcastle Disease Virus in Experimentally Infected Chicken Embryonated Eggs

“…Plants are well known for their potential effects against various infectious diseases in poultry (Abbas et al, 2017a(Abbas et al, , 2017b(Abbas et al, , 2017cHussain et al, 2017), likewise, Nigella sativa is a well-known herbal plant with antiviral, antibacterial effect and have significant role in reduction of viral load in the body (Barakat et al, 2013). The Nigella sativa improves the immunity and work as potent antiviral as described by Zaheer et al (2008).…”