Anticoagulant low molecular weight fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum nodosum

Nardella, Alain; Chaubet, Frédéric; Boisson-Vidal, Catherine; Blondin, Catherine; Durand, Patrick; Jozefonvicz, J.

doi:10.1016/0008-6215(96)00110-3

Cited by 149 publications

(85 citation statements)

References 25 publications

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“…LMWF (4 kDa), isolated and fractionated as described by Nardella et al (1996), was a gift from Institut Française de Recherche pour l'Exploitation de la Mer (Nantes, France). Unfractionated heparin (average molecular mass, 16 kDa) was from Sanofi Center (Toulouse, France), and LMWH (Dalteparin, 5 kDa) was from Pharmacia (Guyancourt, France).…”

Section: Methodsmentioning

confidence: 99%

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Chabut¹,

Fischer²,

Colliec-Jouault³

et al. 2003

Mol Pharmacol

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Basic fibroblast growth factor (FGF-2) activates its high-affinity receptors (FGFRs) but also acts through interaction with heparan sulfate proteoglycans (HSPG). Exogenous polysaccharides also modulate the angiogenic activity of FGF-2. We investigated the effect and mechanism of action of a low molecular weight fucoidan derivative (LMWF) on tube formation by human endothelial cells. LMWF has a better arterial antithrombotic potential in animals than low molecular weight heparin (LMWH). After stimulation of human umbilical vein endothelial cells (HUVEC) by FGF-2 and LMWF (or LMWH), we observed 1) using flow cytometry, an increase in the amount of the ␣6 integrin subunit; 2) using quantitative reverse transcriptionpolymerase chain reaction, an increase in ␣6 mRNA (higher with LMWF than with LMWH); and 3) using a Matrigel model, an increase in vascular tube formation (also higher with LMWF than with LMWH). A direct link between ␣6 overexpression and vascular tube formation was confirmed by use of an anti-␣6 antibody: in its presence, there was no capillary network formation on Matrigel. Unexpectedly, an anti-FGFR blocking antibody had no effect on ␣6 over-expression, whereas stripping off the heparan sulfate with heparitinases abolished overexpression. Overall, our data suggest that FGF-2 stimulates ␣6 over-expression in HUVEC, through HSPG but independently from FGFR, and that LMWF (or LMWH) modulates this interaction. Expression of heparan sulfate proteoglycan increases after ischemic injury. Given its antithrombotic properties and its ability to potentiate tube formation of endothelial cells, LMWF may have to be considered for revascularization of ischemic areas.

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Section: Methodsmentioning

confidence: 99%

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Chabut¹,

Fischer²,

Colliec-Jouault³

et al. 2003

Mol Pharmacol

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“…LMW fucoidan was obtained by radical processing of HMW extracts from brown seaweed (Nardella et al, 1996). Based of previously reported analytical methods (Chevolot et al, 1999), the characteristics of LMW fucoidan were: weight-average molecular mass, 7 Ϯ 2 kDa (polydispersity 1.7); fucose content, 35% (w/w); uronic acid content, 3% (w/w); and sulfate content, 34% (w/w).…”

Section: Lmw Fucoidan Extractionmentioning

confidence: 99%

“…A fraction of low-molecular-weight (LMW) fucoidan (7 Ϯ 2 kDa) was obtained by radical depolymerization of HMW extracts from brown seaweed (Nardella et al, 1996) and was devoid of any direct antithrombin effect (Haroun-Bouhedja et al, 2000). In this study, we have tested the ability of LMW fucoidan to potentiate the effect of FGF-2 in vitro and to mobilize SDF-1 in vivo and have assessed the in vivo therapeutic effect using a rat model of critical hindlimb ischemia previously developed in our laboratory (Luyt et al, 2000).…”

mentioning

confidence: 99%

Low-Molecular-Weight Fucoidan Promotes Therapeutic Revascularization in a Rat Model of Critical Hindlimb Ischemia

Luyt¹,

Meddahi‐Pellé²,

Ho‐Tin‐Noé³

et al. 2003

J Pharmacol Exp Ther

121

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The therapeutic potential of low-molecular-weight (LMW) fucoidan, a sulfated polysaccharide extracted from brown seaweed devoid of direct antithrombin effect, was investigated in vitro and in a model of critical hindlimb ischemia in rat. In vitro results showed that LMW fucoidan enhanced fibroblast growth factor (FGF)-2-induced [ 3 H]thymidine incorporation in cultured rat smooth muscle cells. Intravenous injection in rats of LMW fucoidan significantly increased the stromal-derived factor (SDF)-1 level from 1.2 Ϯ 0.1 to 6.5 Ϯ 0.35 ng/ml in plasma. The therapeutic effect of LMW fucoidan (5 mg/kg/day), FGF-2 (1 g/kg/day), and LMW fucoidan combined with FGF-2 was assessed 14 days after induction of ischemia by 1) clinical evaluation of claudication, 2) tissue blood flow analysis, 3) histoenzymology of muscle metabolic activity, and 4) quantification of capillary density. Both LMW fucoidan and FGF-2 similarly improved residual muscle blood flow (62.5 Ϯ 6.5 and 64.5 Ϯ 4.5%, respectively) compared with the control group (42 Ϯ 3.5%, p Ͻ 0.0001). The combination of FGF-2 and LMW fucoidan showed further significant improvement in tissue blood flow (90.5 Ϯ 3%, p Ͻ 0.0001). These results were confirmed by phosphorylase activity, showing muscle regeneration in rats treated with the combination of FGF-2 and LMW fucoidan. Capillary density count increased from 9.6 Ϯ 0.7 capillaries/muscle section in untreated ischemic controls to 14.3 Ϯ 0.9 with LMW fucoidan, 14.5 Ϯ 0.9 with FGF-2, and 19

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“…Previously, it was shown that fucoidans bind to some growth factors (e.g., HGF, FGFs) and stabilize them (28).…”

Section: Figure 5 Light Microscopic Examination Of Rats' Burn Woundsmentioning

confidence: 99%

Investigation of effects of fucoidan polysaccharides extracted from twospecies of Padina on the wound-healing process in the rat

Kordjazi¹,

Shábanpour²,

Zabihi³

et al. 2017

Turk J Vet Anim Sci

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Seaweeds contains different types of polysaccharides with high abilities. The aim of this study was to determine the potential of fucoidan polysaccharides for wound-healing properties. Fucoidans of two species of Padina (P. tetrastromatica and P. boergesenii), collected from the Persian Gulf northern coastal area, were extracted and analyzed using high-performance anion-exchange chromatography. Purified fucoidans were used to prepare a 2% topical ointment. Under aseptic conditions, two identical paravertebral full-thickness skin wounds (either by burn or excision) were made in 40 anesthetized male rats allocated into 4 groups. Either fucoidan ointments or the vehicle (ointment base) were applied to rats' wounds twice daily for 14 days. In addition to the examination of wound area reduction (days 3, 7, 10, and 14) and histopathological assays (days 7 and 10), tissue specimens were taken to the laboratory. Fucoidans obtained from both seaweeds induced significant wound area reduction compared to the vehicle at some time points after treatment. Histopathological examinations showed improvement in some healing signs including angiogenesis, collagen fiber formation, and epidermis formation. In conclusion, both species of Padina have significant wound-healing effects and fucoidan polysaccharides are the active constituents for this healing property. However, fucoidan obtained from P. boergesenii showed superior effects in this regard.

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Anticoagulant low molecular weight fucans produced by radical process and ion exchange chromatography of high molecular weight fucans extracted from the brown seaweed Ascophyllum nodosum

Cited by 149 publications

References 25 publications

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 Overexpression

Low-Molecular-Weight Fucoidan Promotes Therapeutic Revascularization in a Rat Model of Critical Hindlimb Ischemia

Investigation of effects of fucoidan polysaccharides extracted from twospecies of Padina on the wound-healing process in the rat

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