Investigation of effects of fucoidan polysaccharides extracted from twospecies of Padina on the wound-healing process in the rat

Abstract

Seaweeds contains different types of polysaccharides with high abilities. The aim of this study was to determine the potential of fucoidan polysaccharides for wound-healing properties. Fucoidans of two species of Padina (P. tetrastromatica and P. boergesenii), collected from the Persian Gulf northern coastal area, were extracted and analyzed using high-performance anion-exchange chromatography. Purified fucoidans were used to prepare a 2% topical ointment. Under aseptic conditions, two identical paravertebral full-thickness skin wounds (either by burn or excision) were made in 40 anesthetized male rats allocated into 4 groups. Either fucoidan ointments or the vehicle (ointment base) were applied to rats' wounds twice daily for 14 days. In addition to the examination of wound area reduction (days 3, 7, 10, and 14) and histopathological assays (days 7 and 10), tissue specimens were taken to the laboratory. Fucoidans obtained from both seaweeds induced significant wound area reduction compared to the vehicle at some time points after treatment. Histopathological examinations showed improvement in some healing signs including angiogenesis, collagen fiber formation, and epidermis formation. In conclusion, both species of Padina have significant wound-healing effects and fucoidan polysaccharides are the active constituents for this healing property. However, fucoidan obtained from P. boergesenii showed superior effects in this regard.

Wound dressings on the basis of seaweed polysaccharides, promising for the treatment of burn wounds

“…Kordjazi и соавт. одними из первых изучили ранозаживляющие свойства фукоиданов в эксперименте на ожоговых ранах, показали, что ПС из водорослей Padina tetrastomatic и Padina boergesenii индуцировали образование коллагена и регенерацию эпидермиса [13].…”

Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo

“…The wound healing effects of SPs from P. boergesenii (32.6% sulfate content) and P. tetrastromatica (19% sulfate content) indicate that superior healing activity was correlated with high sulfate content, which manifests as the degree of activation of fibroblast proliferation and function. [38] Moreover, a previous study examining the differential repairing effects of SPs from G. lemaneiformis at various molecular weights found that higher molecular weight limited the physical properties of SPs, such as their highly compact construction and massive molecular size, resulting in decreased possibility of migration to the cell membrane to exert their biological effects. [39] These results were confirmed in similar subsequent studies that SPs with low-molecular weight and increased sulfate levels accelerate dermal wound healing activity in excision rat model.…”

Marine Polysaccharides for Wound Dressings Application: An Overview

“…The results showed that fucoidan promoted dermal papillae re-surfacing and re-epithelialisation [142]. Wound dressings incorporating fucoidan exhibit a variety of abilities to optimise the healing process, including promoting collagen formation, promoting follicle regeneration, reducing inflammatory responses, reducing scar formation, and promoting angiogenesis [143][144][145][146]. Fucoidan and its derivatives are efficient in scavenging hydroxyl radicals and DPPH, exhibiting good antioxidant properties [147].…”