Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon

Chagas, Andrezza Campos; Medeiros, Jansen Fernandes; Astolfi-Filho, Spártaco; Py-Daniel, Victor

doi:10.1590/s0074-02762010000200011

Cited by 6 publications

(1 citation statement)

References 37 publications

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“…Kunitz-domain proteins were seen in all black fly sialotranscriptomes. Probably this family is responsible for the anticoagulant activity previously related to SGHs in S. guianense [137]. …”

Section: Discussionmentioning

confidence: 99%

An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

et al. 2011

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BackgroundLittle is known about the composition and function of the saliva in black flies such as Simulium guianense, the main vector of river blindness disease in Brazil. The complex salivary potion of hematophagous arthropods counteracts their host's hemostasis, inflammation, and immunity.ResultsTranscriptome analysis revealed ubiquitous salivary protein families--such as the Antigen-5, Yellow, Kunitz domain, and serine proteases--in the S. guianense sialotranscriptome. Insect-specific families were also found. About 63.4% of all secreted products revealed protein families found only in Simulium. Additionally, we found a novel peptide similar to kunitoxin with a structure distantly related to serine protease inhibitors. This study revealed a relative increase of transcripts of the SVEP protein family when compared with Simulium vittatum and S. nigrimanum sialotranscriptomes. We were able to extract coding sequences from 164 proteins associated with blood and sugar feeding, the majority of which were confirmed by proteome analysis.ConclusionsOur results contribute to understanding the role of Simulium saliva in transmission of Onchocerca volvulus and evolution of salivary proteins in black flies. It also consists of a platform for mining novel anti-hemostatic compounds, vaccine candidates against filariasis, and immuno-epidemiologic markers of vector exposure.

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Section: Discussionmentioning

confidence: 99%

An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

et al. 2011

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Glandular Matrices and Secretions: Blood-Feeding Arthropods

Mans

2016

Extracellular Composite Matrices in Arthropods

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Ocular manifestations of systemic disease: ocular parasitosis

Rathinam¹,

Kawali²

2010

Current Opinion in Ophthalmology

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Much has been achieved; however, much more effort is needed in the area of translational research from bench to bedside. There is a need to enhance the awareness of risk factors of parasitic diseases in the population. Newer molecular diagnostic techniques need to be standardized for field application. Steps needed to be taken by the ophthalmologist when a parasite is seen in ocular tissues including identification, search for systemic involvement, treatment for elimination and sequelae and public health notification. Lack of methodological uniformity in management emphasis the need for standardization including construction of management algorithm for ophthalmologists.

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Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon

Abstract: In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs)

Cited by 6 publications

References 37 publications

An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

Glandular Matrices and Secretions: Blood-Feeding Arthropods

Ocular manifestations of systemic disease: ocular parasitosis

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