Anticancer Potential of Cratoxylum formosum Subsp. Pruniflorum (Kurz.) Gogel Extracts Against Cervical Cancer Cell Lines

Promraksa, Bundit; Daduang, Jureerut; Khampitak, Tueanjit; Tavichakorntrakool, Ratree; Koraneekit, Amonrat; Palasap, Adisak; Tangrassameeprasert, Roongpet; Boonsiri, Patcharee

doi:10.7314/apjcp.2015.16.14.6117

Cited by 8 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vero cells, isolated from African green monkey kidney epithelial cells, was used as normal control instead of normal human cervical cells due to ethical issues, which is a limitation in the present study. However, previous studies (30,31) have also used Vero cells as control. DMSO is often used in biological studies for solubilizing drugs or studied compounds.…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of cisplatin-caffeic acid induces apoptosis in human cervical cancer cells via the mitochondrial pathways

et al. 2018

Self Cite

View full text Add to dashboard Cite

Cervical cancer (CxCa) is a major health problem globally and is associated with the presence of human papillomavirus infection. Cisplatin (CDDP) is a platinum-based chemotherapeutic agent. Owing to its side effects and drug-resistance, novel anticancer agents with lower toxicity, including caffeic acid (CFC), are of interest. However, the effects of CDDP and CFC in combination are, to the best of our knowledge, uninvestigated. The present study investigated the effectiveness of CDDP and CFC in combination and its mechanism of action on four human cervical cancer cell lines, which were compared with the normal kidney Vero cell line. Cell viability was evaluated using a sulforhodamine B assay. Caspase-Glo assay kits, measuring the activity of caspases-3, -7, -8 and -9, were used to detect caspase activation in HeLa and CaSki cell lines in response to CDDP and CFC in combination. The results revealed that CDDP and CFC alone reduced the proliferation of HeLa, CaSki, SiHa and C33A cell lines. Treatment with CFC exhibited no significant cytotoxicity towards Vero cells. In addition, CDDP-CFC significantly inhibited cell growth of HeLa and CaSki cell lines. In HeLa and CaSki cell lines, a combination index<1 for CDDP and CFC indicated the synergistic growth inhibition; the combination of the two also significantly increased expression of caspase-3, -7 and -9. In conclusion, CFC may be a candidate anticancer agent that, when use in combination, may increase the therapeutic efficacy of CDDP.

show abstract

Section: Discussionmentioning

confidence: 99%

Synergistic effects of cisplatin-caffeic acid induces apoptosis in human cervical cancer cells via the mitochondrial pathways

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…CF appeared to inhibit the growth of HepG2 cancer cells. Previous in vitro studies revealed that CF inhibits growth and induces apoptosis in numerous types of cancer cell, including liver (18), oral (24), bile duct (19) and cervical (25). Senggunprai et al (19) demonstrated that CF was potently cytotoxic against CCA KKU-M156 cells, induced cell apoptosis and inhibited colony formation.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

2017

View full text Add to dashboard Cite

Abstract. The aim of the present study was to investigate the molecular mechanisms underlying Cratoxylum formosum (CF) Dyer-induced cancer cell death and antimigratory effects in HepG2 liver cancer cells. The cytotoxic, antiproliferative and antimigratory effects of CF leaf extract on human liver cancer HepG2 cell lines were evaluated using sulforhodamine B, colony formation, and wound healing assays. In addition, apoptosis induction mechanisms were investigated via reactive oxygen species (ROS) formation, caspase 3 activities, and mitochondrial membrane potential (ΔΨm) disruption. Gene expression and apoptosis-associated protein levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting. CF induced HepG2 cell death in a time-and dose-dependent manner with half maximal inhibitory concentration values of 219.03±9.96 and 124.90±6.86 µg/ml at 24 and 48 h, respectively. Treatment with CF caused a significant and dose-dependent decrease in colony forming ability and cell migration. Furthermore, the present study demonstrated that CF induced ROS formation, increased caspase 3 activities, decreased the ΔΨm, and caused HepG2 apoptosis. CF marginally decreased the expression level of the cell cycle regulatory protein, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) and the downstream protein, cyclin dependent kinase 6. Additionally, CF significantly enhanced p21 levels, reduced cyclin D1 protein levels and triggered cancer cell death. CF leaf extracts induced cell death, stimulated apoptosis and inhibited migration in HepG2 cells. Thus, CF may be useful for developing an anticancer drug candidate for the treatment of liver cancer. IntroductionMedicinal plants and their active compounds have been reported to exert potent cytotoxic activity against various types of cancer cell (1). Numerous plants are consumed as food and are claimed by practitioners of traditional medicine to promote health (2-4). Northeast Thai vegetables, such as Cratoxylum formosum (CF) Dyer may have an affect on human health by exerting antioxidant and anticancer effects. CF belongs to the Guttiferae family and is a plant of the tropics, cultivated in many Southeast Asian countries, including Thailand (5). It is a local dietary and herbal plant, and its leaves are usually consumed fresh. CF has been adopted in folk medicine for the treatment of fever, coughs, stomach ache and peptic ulcers (6,7). CF produces various secondary metabolites, including phenolic compounds, triterpenoids, flavonoids (6,8), xanthones, anthraquinones (7), chlorogenic acid, dicaffeoylquinic acids, and ferulic acid (5). Kukongviriyapan et al (9) detected potent antioxidant activity in aqueous extracts of CF leaves. Other bioactivities demonstrated by CF include anti-inflammatory (10), antibacterial (11), antimicrobial (12) and anticancer (13). Various parts of the plant have been found to exert anticancer effects, including the roots, bark and leaves (14-16).CF inhibits the proliferation of v...

show abstract

“…From our previous report, CEE and CME had cytotoxic effect on both HPV-infected (HeLa and SiHa) and HPV-non infected (C-33A) cervical cancer cell lines. CEE contained gallic acid, resveratrol and quercetin (Promraksa et al, 2015). Gallic acid and quercetin can inhibit migration and invasion of oral squamous cell carcinoma, SCC-4 and SAS, through inhibition of NF-κB and matrix metalloproteinase-2 and -9 (Lai et al, 2013;Kuo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of Cratoxylum Formosum Subsp. Pruniflorum Gogel Extracts in Oral Cancer Cell Lines

Promraksa

Daduang²,

Chaiyarit³

et al. 2015

Asian Pacific Journal of Cancer Prevention

Self Cite

View full text Add to dashboard Cite

Background: Oral cancer is a health problem in Thailand. Cratoxylum formosum subsp. pruniflorum Gogel (Teawdang), normally consumed in northeast Thailand, has proven cytotoxic to cervical cancer cell lines including HeLa, SiHa and C-33A. Recently, Asian oral cancer cell lines, ORL-48 and ORL-136, were established. Therefore, we aimed to study cytotoxicity of Teawdang in these. Total phenolic (TPC) and flavonoid content (TFC), and antioxidant activity of Teawdang were also determined. Materials and Methods: Teawdang was purchased from Khon Kaen market during June-October 2013. Hexane (CHE), ethyl acetate (CEE) and methanol (CME) extracts of its edible part were analyzed for TPC by the folin-ciocalteau method and for TFC by an aluminium colorimetric method. Antioxidant activity and cytotoxicity in normal Vero cells and oral cancer cells were investigated. Cell viability was assessed using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Results: CME and CEE had higher TPC and TFC and antioxidant activity than CHE. Both CME and CEE, at 200 µg dry wt/mL, were cytotoxic to the studied oral cancer cell lines. However, CME was cytotoxic to Vero cells whereas CEE was not. Compared to Vero cells, CEE significantly inhibited ORL-48 and ORL-136 growth (p=0.03 and p=0.02, respectively). Conclusions: CEE exhibited cytotoxic effects on the studied oral cancer cell lines but not normal Vero cells. The bioactive compounds in CEE should be further purified and elucidated for their mechanisms of action for development as anticancer agents.

show abstract

Anticancer Potential of Cratoxylum formosum Subsp. Pruniflorum (Kurz.) Gogel Extracts Against Cervical Cancer Cell Lines

Cited by 8 publications

References 27 publications

Synergistic effects of cisplatin-caffeic acid induces apoptosis in human cervical cancer cells via the mitochondrial pathways

Synergistic effects of cisplatin-caffeic acid induces apoptosis in human cervical cancer cells via the mitochondrial pathways

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

Cytotoxicity of Cratoxylum Formosum Subsp. Pruniflorum Gogel Extracts in Oral Cancer Cell Lines

Contact Info

Product

Resources

About