Cytotoxicity of Cratoxylum Formosum Subsp. Pruniflorum Gogel Extracts in Oral Cancer Cell Lines

Promraksa, Bundit; Daduang, Jureerut; Chaiyarit, Ponlatham; Tavichakorntrakool, Ratree; Khampitak, Tueanjit; Rattanata, Narintorn; Tangrassameeprasert, Roongpet; Boonsiri, Patcharee

doi:10.7314/apjcp.2015.16.16.7155

Cited by 9 publications

(6 citation statements)

References 32 publications

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“…CF appeared to inhibit the growth of HepG2 cancer cells. Previous in vitro studies revealed that CF inhibits growth and induces apoptosis in numerous types of cancer cell, including liver (18), oral (24), bile duct (19) and cervical (25). Senggunprai et al (19) demonstrated that CF was potently cytotoxic against CCA KKU-M156 cells, induced cell apoptosis and inhibited colony formation.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

2017

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Abstract. The aim of the present study was to investigate the molecular mechanisms underlying Cratoxylum formosum (CF) Dyer-induced cancer cell death and antimigratory effects in HepG2 liver cancer cells. The cytotoxic, antiproliferative and antimigratory effects of CF leaf extract on human liver cancer HepG2 cell lines were evaluated using sulforhodamine B, colony formation, and wound healing assays. In addition, apoptosis induction mechanisms were investigated via reactive oxygen species (ROS) formation, caspase 3 activities, and mitochondrial membrane potential (ΔΨm) disruption. Gene expression and apoptosis-associated protein levels were measured by reverse transcription-quantitative polymerase chain reaction and western blotting. CF induced HepG2 cell death in a time-and dose-dependent manner with half maximal inhibitory concentration values of 219.03±9.96 and 124.90±6.86 µg/ml at 24 and 48 h, respectively. Treatment with CF caused a significant and dose-dependent decrease in colony forming ability and cell migration. Furthermore, the present study demonstrated that CF induced ROS formation, increased caspase 3 activities, decreased the ΔΨm, and caused HepG2 apoptosis. CF marginally decreased the expression level of the cell cycle regulatory protein, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) and the downstream protein, cyclin dependent kinase 6. Additionally, CF significantly enhanced p21 levels, reduced cyclin D1 protein levels and triggered cancer cell death. CF leaf extracts induced cell death, stimulated apoptosis and inhibited migration in HepG2 cells. Thus, CF may be useful for developing an anticancer drug candidate for the treatment of liver cancer. IntroductionMedicinal plants and their active compounds have been reported to exert potent cytotoxic activity against various types of cancer cell (1). Numerous plants are consumed as food and are claimed by practitioners of traditional medicine to promote health (2-4). Northeast Thai vegetables, such as Cratoxylum formosum (CF) Dyer may have an affect on human health by exerting antioxidant and anticancer effects. CF belongs to the Guttiferae family and is a plant of the tropics, cultivated in many Southeast Asian countries, including Thailand (5). It is a local dietary and herbal plant, and its leaves are usually consumed fresh. CF has been adopted in folk medicine for the treatment of fever, coughs, stomach ache and peptic ulcers (6,7). CF produces various secondary metabolites, including phenolic compounds, triterpenoids, flavonoids (6,8), xanthones, anthraquinones (7), chlorogenic acid, dicaffeoylquinic acids, and ferulic acid (5). Kukongviriyapan et al (9) detected potent antioxidant activity in aqueous extracts of CF leaves. Other bioactivities demonstrated by CF include anti-inflammatory (10), antibacterial (11), antimicrobial (12) and anticancer (13). Various parts of the plant have been found to exert anticancer effects, including the roots, bark and leaves (14-16).CF inhibits the proliferation of v...

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

2017

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“…1B). The TFC values in the fractions had a similar pattern to the TPC, being higher in extracts from a high polarity solvent (Esmaeili et al, 2015;Promraksa et al, 2015). However, flavonoids can also be found in extract with a low polarity solvent, as reported in the extraction from leaves of Vitex doniana (Yakubu et al, 2014), where the highest TFC (390.00 mg/mL of quercetin) was in chloroform extract.…”

Section: Total Flavonoids Contentmentioning

confidence: 60%

“…Trifolium pretense L. and of C. formosum leaves, which had a higher TPC in the methalonic extract than for the extracts with any other solvents (Esmaeili et al, 2015;Promraksa et al, 2015). More phenolic compounds were found in high polarity solvents than low polarity solvents because the hydroxyl group in the phenolic compounds creates polarity and allows the compounds to dissolve more readily in a high polarity solvent (Galanakis et al, 2013).…”

Section: Extraction and Fractionation Of Stem Bark Materialsmentioning

confidence: 99%

Isolation of betulinic acid and antioxidant and anti-HIV-1 reverse transcriptase activity of Cratoxylum formosum subsp. pruniflorum (Kurz) Gogelein extract

2019

ANRES

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Cratoxylum formosum subsp. pruniflorum (Kurz) Gogelein has been used as a medicinal plant for the treatment of many diseases. C. formosum bark was macerated with methanol and partitioned using hexane and chloroform. The chloroform bark extract was fractionated using column chromatography. Five combined fractions were obtained (CFA-CFE) and then investigated for antioxidant activity based on the total phenolic content (TPC), total flavonoids content (TFC), ferric reducing antioxidant power (FRAP) and nitric oxide (NO) radical scavenging activity and also for anti-HIV-1 reverse transcriptase (RT) activity. CFE had the highest antioxidant activity, with values for TPC, TFC and FRAP of 44.84 ± 0.05 μg of gallic acid equivalents/g, 580.69 ± 0.78 μg of quercetin equivalents/g and 82.56 ± 0.08 μg of Trolox equivalents/g of dry weight extract, respectively, and CFB inhibited the nitric oxide radical at the lowest concentration (96.73 μg/mL). However, the methanolic bark extract, fractions CFC, CFD and CFE had high inhibitory effects against HIV-1 RT with values greater than 60% relative inhibition. CFE had the highest percentage relative inhibition among the fractions and was at a comparable level to a standard drug (Nevirapine). The ranking of the antioxidant and anti-HIV-1 RT activities of the fractions was CFE followed by CFD, CFC, CFB and CFA, respectively. Betulinic acid was isolated from fraction 52 of the chloroform extract that was eluted with chloroform-acetone (1:9, volume per volume). The 1 H and 13 C nuclear magnetic resonance data obtained were in accordance with the previous published data. There is a need for further isolation of bioactive compounds and evaluation of their antioxidant and anti-HIV activities.

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“…The measurement was carried out in triplicate. The calculation was performed as described in a previous report [28].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of anticancer potential of Thai medicinal herb extracts against cholangiocarcinoma cell lines

et al. 2019

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Although cholangiocarcinoma (CCA) has a low incidence globally, this is extremely high in Northeast Thailand. The lack of both early detection measures and effective therapeutic drugs is the major problem for the poor prognosis of CCA patients. Based on regional knowledge, it would be advantageous to search for effective natural phyto-products for the treatment of CCA. Cardiospermum halicacabum L., Gomphrena celosioides Mart. and Scoparia dulcis L., very well-known medicinal herbs in Asian countries, were selected for the investigation of inhibitory effects on CCA cells. Of the three different ethanolic extracts, S . dulcis L extract showed most inhibitory effects on cell growth of CCA cell lines KKU-100 and KKU-213, at percentages of 56.06 and 74.76, respectively, compared to the untreated group after treatment with 250 μg/mL of extracts for 72 hrs. At 400 and 500 μg/mL of the extracts, the inhibitory effect of KKU-213 was indicated by a significant increase in the BAX/Bcl-2 ratio and cell membrane permeability. Moreover, metabolic profiling-based screening employed in the current study revealed a significant positive association between the lignin compound and a decrease in CCA cell viability. Our study suggests, for the first time, that ESD has the ability to inhibit CCA cell growth through the induction of apoptosis.

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Cytotoxicity of Cratoxylum Formosum Subsp. Pruniflorum Gogel Extracts in Oral Cancer Cell Lines

Cited by 9 publications

References 32 publications

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

Cytotoxic and antimigratory effects of Cratoxy formosum extract against HepG2 liver cancer cells

Isolation of betulinic acid and antioxidant and anti-HIV-1 reverse transcriptase activity of Cratoxylum formosum subsp. pruniflorum (Kurz) Gogelein extract

Evaluation of anticancer potential of Thai medicinal herb extracts against cholangiocarcinoma cell lines

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