Anticancer activity of uncaria gambir roxb on T47D breast cancer cells

Abstract

Breast cancer was the second most common cancer that causes death in women worldwide. One ingredients of herbal plants as an alternative therapy in the community, was gambir leaves. This research was conducted to examine the anticancer activity of Uncaria gambir Roxb on T47D breast cancer cells in vitro. Six levels of concentration U. gambir (31.25 mg/ml up to 1000 mg/ml) was included on the 96 microplate well which had been filled with breast cancer cells, the positive control was doxorubicin (with concentration of 0.03-1 mg/ml). The anticancer effects of U.gambir was determined by MTT (3-[4,5-dimethylthiazol-2-yl]- diphenyl tetrazolium bromide 2.5). The results showed the existence of anticancer activity of Uncaria gambir Roxb at concentrations of 250, 500 and 1000 μg/ml. The largest anticancer activity found at a concentration of 500 μg/ml with the percentage of apoptotic cells of breast cancer by 20%, but we also found a decrease in the value of the apoptotic at concentration of 1000 μg/ml. The IC50 of U.gambir was 1.086 μg/ml whereas the IC50 doxorubicin was 0.108 μg/ml. This study concluded that the inhibition of breast cancer cell growth by gambir leaves extract was still quite weak compared to doxorubicin.

Current Research of Phytochemical, Medicinal and Non-Medicinal Uses of Uncaria gambir Roxb.: A Review

“…Gambir, which is rich in flavonoid secondary metabolite compounds, especially catechins, has antioxidant activity that can capture free radicals [ 93 ], having great potential as an anticancer. Syarifah et al [ 14 ] investigated the anticancer activity of gambir on T47D breast cancer cells in vitro. The results showed that gambir inhibited breast cancer cell growth, although this property was still weaker than doxorubicin (DOX) as a positive control.…”

“…With the continuous improvements in medicinal plant research methods, increasing numbers of compounds including alkaloids, flavonoids, and tannins have been discovered from gambir. Modern studies have shown that gambir has pharmacological activities such as anticancer [ 14 ], enzyme inhibitory [ 15 ], and hypoglycemic [ 16 ] activity due to flavonoid compounds [ 17 ].…”

Review: Akar Kayu Bajakah dan Manfaatnya untuk Kesehatan

“…Semua senyawa tersebut diklaim mampu mengobati berbagai penyakit degeneratif layaknya diabetes, kanker, tumor, dan lain-lain. Hal ini diperkuat dengan penelitian yang telah di lakukan (Syarifah et al 2019) yang menyatakan bahwa akar bajakah tampala dapat mengobati penyakit kanker. Ayuchecaria et al (2020) menyatakan bahwa ekstrak batang bajakah tampala rata-rata mengandung kadar fenolik sebesar 12,33mg GAE/g.…”

Efek Sitotoksik Beberapa Akar Bajakah Kalimantan Terhadap Sel Kanker Payudara T47d

“…Terdapat 200 jenis bajakah di Indonesia, khususnya di Kalimantan. Meskipun terdapat ratusan jenis bajakah di Indonesia, namun belum ada penelitian ilmiah terkait aktivitas sitotoksisitas dari bajakah (Åkesson et al, 2003;Batiha et al, 2020;Cheng et al, 2007;Stephenson, 1931;Syarifah et al, 2019). Oleh karena itu, perlu adanya usaha…”