Anticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell line

Taştemur, Şeyma; Hacısüleyman, Levent; Karataş, Özhan; Yulak, Fatih; Ataseven, Hilmi

doi:10.1139/cjpp-2022-0523

Cited by 9 publications

(7 citation statements)

References 43 publications

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“…Sinapic acid is abundantly found in different plant species, e.g., strawberries and cranberries, as well as cereals and vegetables; it is one of the most common compounds in the Brassicaceae family. Data found in the literature show that sinapic acid possesses antioxidant and anti-inflammatory properties [3], as well as anticancer [4], hepatoprotective [5], cardioprotective [6], renoprotective [6], neuroprotective [7], antidiabetic [8], anxiolytic [9], and antibacterial activity [10]. A review of the pharmacological and therapeutic applications of sinapic acid was recently published by Pandi et al [11].…”

Section: Methodsmentioning

confidence: 99%

Assessment of Pharmaco-Technological Parameters of Solid Lipid Nanoparticles as Carriers for Sinapic Acid

Russo

Greco

Sarpietro

2023

Micro

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Sinapic acid, 3,5-dimethoxyl-4-hydroxycinnamic acid, belonging to the class of hydroxycinnamic acids, shows antioxidant, anti-inflammatory, anticancer, hepatoprotective, cardioprotective, renoprotective, neuroprotective, antidiabetic, anxiolytic, and antibacterial activity. The aim of this work was to incorporate sinapic acid into solid lipid nanoparticles in order to improve its bioavailability. SLNs were prepared using the hot high-speed homogenization method. The pharmaco-technological properties and thermotropic profile of SLNs encapsulated with sinapic acid, as well as their interaction with biomembrane models, were evaluated. SLNs showed promising physicochemical properties and encapsulation efficiency, as well as a desirable release profile; moreover, they facilitated the interaction of sinapic acid with a biomembrane model made of multilamellar vesicles. In conclusion, this formulation can be used in further studies to assess its suitability to improve sinapic acid activity.

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Section: Methodsmentioning

confidence: 99%

Assessment of Pharmaco-Technological Parameters of Solid Lipid Nanoparticles as Carriers for Sinapic Acid

Russo

Greco

Sarpietro

2023

Micro

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“…Spices, citrus and berry fruits, vegetables, cereals, oilseed crops, and vegetables are among the edible plants that contain the phytochemical sinapic acid [118,119]. Sinapic acid has been investigated and documented in relation to a number of clinical conditions, including infections, oxidative stress [120], inflammation [121], cancer [122], T2D [123], neurodegeneration [124], and anxiety [125]. Studies have also been conducted on the acetylcholinesterase inhibition [126,127], antimutagenic [128], and antioxidant activity [129] of a few sinapic acid derivatives, including sinapine, 4-vinylsyringol, and syringaldehyde.…”

Section: Health Potentialmentioning

confidence: 99%

“…p-Coumaric acid Antimicrobial, Anti-inflammatory, anticancer [108][109][110] Ferulic acid Anticancer, antihypertensive, antidiabetes, anti-inflammatory [111][112][113][114][115] Caffeic acid Antimutagenic, anticarcinogenic [116] Sinapic acid Antioxidative, anti-inflammatory, anticancer, antidiabetic, anti-neurodegeneration, anti-anxiety [120][121][122][123][124][125] p-Hydroxybenzoic acid Antithrombotic and anticoagulant [130,131]…”

Section: Phenolic Acid Health Potential Referencesmentioning

confidence: 99%

Composition of Whole Grain Dietary Fiber and Phenolics and Their Impact on Markers of Inflammation

Khan,

Gul,

Rashid

et al. 2024

Nutrients

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Inflammation is an important biological response to any tissue injury. The immune system responds to any stimulus, such as irritation, damage, or infection, by releasing pro-inflammatory cytokines. The overproduction of pro-inflammatory cytokines can lead to several diseases, e.g., cardiovascular diseases, joint disorders, cancer, and allergies. Emerging science suggests that whole grains may lower the markers of inflammation. Whole grains are a significant source of dietary fiber and phenolic acids, which have an inverse association with the risk of inflammation. Both cereals and pseudo-cereals are rich in dietary fiber, e.g., arabinoxylan and β-glucan, and phenolic acids, e.g., hydroxycinnamic acids and hydroxybenzoic acids, which are predominantly present in the bran layer. However, the biological mechanisms underlying the widely reported association between whole grain consumption and a lower risk of disease are not fully understood. The modulatory effects of whole grains on inflammation are likely to be influenced by several mechanisms including the effect of dietary fiber and phenolic acids. While some of these effects are direct, others involve the gut microbiota, which transforms important bioactive substances into more beneficial metabolites that modulate the inflammatory signaling pathways. Therefore, the purpose of this review is twofold: first, it discusses whole grain dietary fiber and phenolic acids and highlights their potential; second, it examines the health benefits of these components and their impacts on subclinical inflammation markers, including the role of the gut microbiota. Overall, while there is promising evidence for the anti-inflammatory properties of whole grains, further research is needed to understand their effects fully.

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“…The levels these markers in the resulting cell lysates were quanti ed according to the instructions provided by the manufacturer. Furthermore, the total protein concentrations of both the gambogic acid-treated and untreated SNU-16 cells were assessed using the BCA assay from Pierce Biotechnology in the United States [10,11].…”

Section: Cell Viability Assaymentioning

confidence: 99%

Mechanism of Anticancer Effect of Gambogic Acid on Gastric Signet Ring Cell Carcinoma

Joha

Öztürk

Yulak

et al. 2023

Preprint

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Background Gambogic acid has demonstrated inhibitory effects on the growth of various cancer cell types, such as breast cancer, pancreatic cancer, prostate cancer, lung cancer, and osteosarcoma. This study aims to investigate the antiproliferative activity of Gambogic acid on SNU-16 cells derived from gastric signet ring cell carcinoma and elucidate the underlying mechanisms. Material and Methods The cytotoxic effect of gambogic acid was evaluated in SNU-16 cells by treating them with different concentrations of the compound, and the XTT cell viability assay was employed to assess cell viability. ELISA was used to measure bax, BCL-2, caspase 3, PARP, and 8-oxo-dG levels. Additionally, immunofluorescence staining was applied to assess 8-oxo-dG and LC3β levels in SNU-16 cells. Results It was observed that gambogic acid exerted a dose-dependent and statistically significant antiproliferative effect on SNU-16 cells. The IC50 value of gambogic acid in SNU-16 cells was found to be 655.1 nM for 24 hours. Subsequent investigations conducted using the IC50 dose revealed a significant upregulation of apoptotic proteins including cleaved caspase 3, Bax, and cleaved PARP (p < 0.001), along with a downregulation of BCL-2 (p < 0.001), an anti-apoptotic protein. Moreover, the administration of this drug led to an upregulation of 8-oxo-dG (p < 0.001), a widely acknowledged biomarker indicating oxidative damage in DNA, as well as an increase in LC3β levels (p < 0.05), a marker associated with autophagy. Conclusion The antiproliferative effect of gambogic acid against gastric signet ring cell carcinoma is attributed to its ability to induce apoptosis and autophagy. This discovery highlights the promising potential of gambogic acid as a treatment option for gastric signet ring cell carcinoma.

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Anticancer activity of sinapic acid by inducing apoptosis in HT-29 human colon cancer cell line

Cited by 9 publications

References 43 publications

Assessment of Pharmaco-Technological Parameters of Solid Lipid Nanoparticles as Carriers for Sinapic Acid

Assessment of Pharmaco-Technological Parameters of Solid Lipid Nanoparticles as Carriers for Sinapic Acid

Composition of Whole Grain Dietary Fiber and Phenolics and Their Impact on Markers of Inflammation

Mechanism of Anticancer Effect of Gambogic Acid on Gastric Signet Ring Cell Carcinoma

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