Anticancer activity and quantitative analysis of flavone of<b><i>Cirsium japonicum</i></b>DC

Liu, Sujun; Zhang, Jie; Li, Daxu; Liu, Wei; Luo, Xun; Zhang, Rongxian; Li, Li; Zhao, Jian

doi:10.1080/14786410701494686

Cited by 49 publications

(30 citation statements)

References 9 publications

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“…Furthermore, the anticancer activity of the two flavones in S180 and H22 mice was studied and these two flavones greatly inhibited cancer cell growth, and increased the life span. [57][58][59] Cephalotaxus harringtonia Several phytochemicals obtained from the chloroform extract of leaves and stems of Cephalotaxus harringtonia (Cephalotaxaceae) are cephalotaxine and its antitumor esters (harringtonine, isoharringtonine and homoharringtonine) in both callus and medium. It shows significant activity against experimental P388 leukemia and L-121O leukemia in mice.…”

Section: Cirsium Japonicummentioning

confidence: 99%

Medicinal Plants for Treatment of Cancer: A Brief Review

Jain¹,

Dwivedi²,

Jain³

et al. 2016

phcogj

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Section: Cirsium Japonicummentioning

confidence: 99%

Medicinal Plants for Treatment of Cancer: A Brief Review

Jain¹,

Dwivedi²,

Jain³

et al. 2016

phcogj

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“…In plants, flavones play important physiological roles, including UV protection (Schmitz-Hoerner and Weissenböck, 2003), interactions with other organisms (Peters et al, 1986;Kong et al, 2007), regulation of auxin transport (Mathesius et al, 1998), and copigmentation in flowers (Goto and Kondo, 1991). On the other hand, flavones as dietary constituents or supplements have become increasingly popular due to their health-beneficial effects, such as cancer prevention (Bontempo et al, 2007;Cai et al, 2007;Liu et al, 2007), antioxidation (Park et al, 2007), antiviral activities (Yarmolinsky et al, 2012), reduction of risks of cardiovascular diseases (Baek et al, 2009), and suppression of cholesterol levels (Dharmarajan and Arumugam, 2012).…”

mentioning

confidence: 99%

Cytochrome P450 93G1 Is a Flavone Synthase II That Channels Flavanones to the Biosynthesis of Tricin O-Linked Conjugates in Rice

et al. 2014

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Flavones are a major class of flavonoids with a wide range of physiological functions in plants. They are constitutively accumulated as C-glycosides and O-linked conjugates in vegetative tissues of grasses. It has long been presumed that the two structural modifications of flavones occur through independent metabolic routes. Previously, we reported that cytochrome P450 93G2 (CYP93G2) functions as a flavanone 2-hydroxylase (F2H) that provides 2-hydroxyflavanones for C-glycosylation in rice (Oryza sativa). Flavone C-glycosides are subsequently formed by dehydratase activity on 2-hydroxyflavanone C-glycosides. On the other hand, O-linked modifications were proposed to proceed after the flavone nucleus is generated. In this study, we demonstrate that CYP93G1, the closest homolog of CYP93G2 in rice, is a bona fide flavone synthase II (FNSII) that catalyzes the direct conversion of flavanones to flavones. In recombinant enzyme assays, CYP93G1 desaturated naringenin and eriodictyol to apigenin and luteolin, respectively. Consistently, transgenic expression of CYP93G1 in Arabidopsis (Arabidopsis thaliana) resulted in the accumulation of different flavone O-glycosides, which are not naturally present in cruciferous plants. Metabolite analysis of a rice CYP93G1 insertion mutant further demonstrated the preferential depletion of tricin O-linked flavanolignans and glycosides. By contrast, redirection of metabolic flow to the biosynthesis of flavone C-glycosides was observed. Our findings established that CYP93G1 is a key branch point enzyme channeling flavanones to the biosynthesis of tricin O-linked conjugates in rice. Functional diversification of F2H and FNSII in the cytochrome P450 CYP93G subfamily may represent a lineage-specific event leading to the prevalent cooccurrence of flavone C-and O-linked derivatives in grasses today.

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“…recently, it was reported that C. japonicum and its flavone components induce the inhibition of tumor formation in mice (9,10). However, the mechanism underlying the anti-cancer effects of these C. japonicum compounds are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Cirsium japonicum extract induces apoptosis and anti-proliferation in the human breast cancer cell line MCF-7

Kim

Kang²,

Ghil³

2010

Mol Med Rep

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Abstract. Cirsium japonicum is a wild perennial herb that has been used as an anti-hemorrhagic, anti-hypertensive and uretic agent in traditional chinese medicine. recently, it was reported that C. japonicum inhibits the growth of implanted cancer cells. However, the molecular mechanisms underlying the anti-cancer properties of C. japonicum are not fully understood. in this study, we investigated the effect of a methanol extract of C. japonicum on cell growth in the human breast cancer cell line McF-7. C. japonicum extract inhibited the cell proliferation of McF-7 cells in a time-and dose-dependent manner, as evaluated by the MTT assay. Furthermore, C. japonicum extract induced an antiproliferative effect by causing G 1 phase cell cycle arrest and also induced apoptosis by affecting mitochondrial apoptotic events, as determined by nuclear derangement, flow cytometry and Western blot analysis. Taken together, our findings indicate that C. japonicum extract induces the inhibition of McF-7 cell growth at both the proliferation and apoptosis levels. Introductioncurrently, many efforts are underway to develop new therapeutic anti-cancer drugs from natural sources. a major natural reservoir is plant material. Plant secondary metabolites have occasionally been used as chemotherapeutic agents to mediate cytostatic activity in cancer cells. Widely used anti-mitotic anti-cancer drugs including Vinca alkaloids and paclitaxel have been isolated from Catharanthus roseus and Taxus baccata, respectively (1). The dna polymerase i inhibitor camptothecin was sourced from Camptotheca acuminata (2). curcumin isolated from Curcuma longa has shown anticancer properties, such as the inhibition of tumor promotion and the induction of apoptosis in cancer cells (3-5).Cirsium japonicum is a wild perennial herb found in many areas of Korea, Japan and china. it has been used as an anti-hemorrhagic, anti-hypertensive and uretic agent in traditional chinese medicine (6). recent studies have found that the water extracts of C. japonicum induce the activation of estrogen receptors and have estrogenic effects (7). C. japonicum also contains a vasorelaxant principle, mediating histamine H1-receptor activation (8). in traditional medicine, C. japonicum has sometimes been used for the management of different types of cancer, including liver and uterine cancer and leukemia (9).recently, it was reported that C. japonicum and its flavone components induce the inhibition of tumor formation in mice (9,10). However, the mechanism underlying the anti-cancer effects of these C. japonicum compounds are not fully understood.in the present study, we observed that a methanol extract of C. japonicum significantly reduced cancer cell growth in a time-and dose-dependent manner, using the human breast cancer cell line McF-7. Furthermore, we investigated the molecular mechanisms underlying the anti-proliferative activities of C. japonicum. Materials and methodsPreparation of C. japonicum extract. dried roots of C. japonicum from Korea were purchased from the Kyungd...

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Anticancer activity and quantitative analysis of flavone ofCirsium japonicumDC

Cited by 49 publications

References 9 publications

Medicinal Plants for Treatment of Cancer: A Brief Review

Medicinal Plants for Treatment of Cancer: A Brief Review

Cytochrome P450 93G1 Is a Flavone Synthase II That Channels Flavanones to the Biosynthesis of Tricin O-Linked Conjugates in Rice

Cirsium japonicum extract induces apoptosis and anti-proliferation in the human breast cancer cell line MCF-7

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