Antibody to Varicella-Zoster Virus-Induced Membrane Antigen: Immunoperoxidase Assay with Air-Dried Target Cells

Haikin, Hava; Leventon-Kriss, S; Sarov, Israel

doi:10.1093/infdis/140.4.601

Cited by 25 publications

(18 citation statements)

References 14 publications

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“…In all patients, the titre reached a peak during the second and the third weeks of the illness. Most of the subjects (90%) with a past history of varicella had low titres of antibodies against VZV, as reported by other authors (Williams, Gershon and Brunell, 1974;Haikin, Leventon-Kriss and Sarov, 1979).…”

Section: Discussionsupporting

confidence: 80%

Virological course of herpes zoster in otherwise normal hosts

Cevenini

Donati

Rumpianesi

et al. 1983

Journal of Medical Microbiology

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SUMMARY. The virological course of herpes zoster infection in 42 otherwise normal hosts was studied by virus isolation and antibody titration. Varicella-zoster virus (VZV) was isolated from vesicle fluid from all three patients examined on the first day of the vesicular eruption and from five out of six examined on the second day. The isolation rate fell to one out of six patients on the seventh day of illness and VZV was not isolated from patients at a later stage of the illness. IgG antibodies were detected by IFAMA and ELISA, in sera from all the patients by the end of the first week of illness; IgG antibody titres were highest during the second and the third weeks. IgM antibodies to VZV were detected in sera from six of the 42 patients with herpes zoster after fractionation by ion-exchange chromatography.

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Section: Discussionsupporting

confidence: 80%

Virological course of herpes zoster in otherwise normal hosts

Cevenini

Donati

Rumpianesi

et al. 1983

Journal of Medical Microbiology

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“…3 The diagnosis of C trachomatis infections is generally made after isolation in cell culture,4 while the detection of chlamydial antibody in sera or local secretions is possible by other techniques. To Result Fig.…”

mentioning

confidence: 99%

A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

Cevenini¹,

Rumpianesi²,

Donati³

et al. 1983

Journal of Clinical Pathology

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“…In 40, a significant level of antibody of the IgG class against VZV was found by IPAMA [30], while 5 were VZV IgG-negative. All these sera were tested for specific VZV IgM by ELISA and proved negative (< 50).…”

Section: Specificity (I) Sucrose Gradient Analysismentioning

confidence: 99%

“…The immunoperoxidase antibody to membrane antigen (IPAMA) technique for determination of VZV IgG antibody was carried out as described by Haikin et al [30].…”

Section: Pam Amentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay for Detection of Virus-Specific IgM Antibodies to Varicella-Zoster Virus

Hacham

Leventon-Kriss

Sarov³

1980

Intervirology

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A sensitive enzyme-linked immunosorbent assay (ELISA) is described for detection of varicella-zoster virus (VZV) IgM antibodies. The antigen consisted of a sonically disrupted extract of VZV-infected human embryo cells. The tested sera were absorbed with Staphylococcus aureus (strain Cowan I) before analysis. Rabbit anti-human IgM peroxidase conjugate was used to detect human IgM bound to viral antigen. The results were compared with those obtained by the indirect fluorescent antibody to membrane antigen (IFAMA) technique. Comparison of titers obtained by ELISA with those obtained by IFAMA for sera of chickenpox patients showed agreement between the results in 8 of 9 patients. In 1 chickenpox patient, no VZV IgM antibodies could be detected by IFAMA, while a titer of 3,200 was obtained by ELISA. The ELISA technique described gave titers more than 100 times higher than those obtained by IFAMA. VZV IgM antibody was detected by ELISA and IFAMA in only 1 of 5 zoster patients. No VZV IgM antibodies were found by ELISA in 45 control sera (healthy adults and hospitalized patients with various other diseases). Neither were they found in paired sera of 6 patients with acute herpes simplex infections, 2 patients with Epstein-Barr virus infections, and 3 patients with human cytomegalovirus infections.

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Antibody to Varicella-Zoster Virus-Induced Membrane Antigen: Immunoperoxidase Assay with Air-Dried Target Cells

Cited by 25 publications

References 14 publications

Virological course of herpes zoster in otherwise normal hosts

Virological course of herpes zoster in otherwise normal hosts

A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

Enzyme-Linked Immunosorbent Assay for Detection of Virus-Specific IgM Antibodies to Varicella-Zoster Virus

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