Enzyme-Linked Immunosorbent Assay for Detection of Virus-Specific IgM Antibodies to Varicella-Zoster Virus

Hacham, Moshe; Leventon-Kriss, S; Sarov, Israel

doi:10.1159/000149128

Cited by 24 publications

(10 citation statements)

References 11 publications

(12 reference statements)

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“…The procedure is a modification [20][21][22] of that described by Bidwell et al [23]. The assay was carried out on Nunc polystyrene microtest plates (96U-1182 1; Nunc, Denmark).…”

Section: Elisamentioning

confidence: 99%

Detection of Specific IgA Antibodies in Serum of Kidney Transplant Patients with Recurrent Cytomegalovirus Infection

et al. 1981

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59 sera of 10 immunosuppressed renal allograft recipients who experienced recurrent cytomegalovirus (CMV) infection were analyzed by enzyme-linked immunosorbent assay (ELISA) for CMV IgA antibodies and by the complement-fixation (CF) test. A significant rise of CF titer was evident 4–53 weeks post-transplantation. 9 patients produced CMV IgA in high titers at about the time the CF antibody rise was observed. 1 patient did not produce IgA antibodies to CMV. In 3 of the 9 patients, specific CMV IgA antibodies were detected before the rise in CF titer was demonstrated. CMV IgA antibodies were found to persist for as long as 66 weeks post-transplantation. The potential application of ELISA detection of CMV-specific IgA antibodies as an early indication of CMV infection in kidney transplant patients is discussed.

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“…The procedure is a modification [20][21][22] of that described by Bidwell et al [23]. The assay was carried out on Nunc polystyrene microtest plates (96U-1182 1; Nunc, Denmark).…”

Section: Elisamentioning

confidence: 99%

Detection of Specific IgA Antibodies in Serum of Kidney Transplant Patients with Recurrent Cytomegalovirus Infection

et al. 1981

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“…This test, however, is not very sensitive for determination of past infections and requires detection of a significant antibody 166 A. M. VAN LOON AND OTHERS titre rise in paired serum specimens for diagnosis of acute infection. It has been shown previously [1][2][3][4][5][6][7][8] that detection of VZV-specific immunoglobulin M (VZVIgM) antibody allows rapid diagnosis of acute VZV infection. In all patients with varicella, VZV-IgM was found early in the disease using a variety of methods including the indirect immunofluorescence assay [2], the indirect enzyme-linked immunosorbent assay (ELISA) [3] as well as antibody-capture assays using radioactive [4,5,8] or enzyme labels [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown previously [1][2][3][4][5][6][7][8] that detection of VZV-specific immunoglobulin M (VZVIgM) antibody allows rapid diagnosis of acute VZV infection. In all patients with varicella, VZV-IgM was found early in the disease using a variety of methods including the indirect immunofluorescence assay [2], the indirect enzyme-linked immunosorbent assay (ELISA) [3] as well as antibody-capture assays using radioactive [4,5,8] or enzyme labels [6,7]. In contrast, in patients with herpes zoster an IgM response has been reported in between 20% and 98% of patients [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…In all patients with varicella, VZV-IgM was found early in the disease using a variety of methods including the indirect immunofluorescence assay [2], the indirect enzyme-linked immunosorbent assay (ELISA) [3] as well as antibody-capture assays using radioactive [4,5,8] or enzyme labels [6,7]. In contrast, in patients with herpes zoster an IgM response has been reported in between 20% and 98% of patients [1][2][3][4][5][6][7][8]. Therefore the value of VZV-IgM detection for rapid diagnosis of herpes zoster is less clear.…”

Section: Introductionmentioning

confidence: 99%

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Antibody-capture enzyme-linked immunosorbent assays that use enzyme-labelled antigen for detection of virus-specific immunoglobulin M, A and G in patients with varicella or herpes zoster

Loon¹,

Logt²,

Heessen³

et al. 1992

Epidemiol. Infect.

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SUMMARYAntibody-capture enzyme-linked immunosorbent assays (AC-ELISA) which use enzyme-labelled antigen were developed for detection of varicella-zoster virus-(VZV) specific IgM, IgA and IgG antibody in patients with varicella or herpes zoster and in sera from healthy individuals. All 18 patients with varicella developed a VZV-IgM and a VZV-IgG response, 17 also a VZV-IgA response. In contrast, all 19 patients with herpes zoster were shown to be positive for VZV-IgA whereas only 13 of these reacted positively for VZV-IgM. A VZV-IgM response was detected in only two sera from 100 healthy individuals and an IgA response in only one. The presence of virus-specific IgA and IgG in the cerebrospinal fluid as determined by AC-ELISA was a useful indicator of VZV infection of the central nervous system. By AC-ELISA, VZV-IgG was detected predominantly in sera from patients with acute or recent VZV infection. Only 14 sera from 100 healthy individuals were positive for VZV-IgG by AC-ELISA, whereas all were positive by an indirect ELISA. These results indicate that AC-ELISA's may be useful assays for determination for acute or recurrent VZV infection, but are not suitable for determination of past infection with this virus.

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“…IgG responses are characteristic of secondary antigenic stimulation but IgM responses have been reported in herpes zoster (Ross and McDaid, 1972;Arvin and Koropchak, 1980;Hacham, Leventon-Kriss and Sarov, 1980;Tedder, Mortimer and Lord, 1981). However, other authors could not detect specific anti-VZV IgM (Schmidt and Lennette, 1975;Gerna et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

Virological course of herpes zoster in otherwise normal hosts

Cevenini

Donati

Rumpianesi

et al. 1983

Journal of Medical Microbiology

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SUMMARY. The virological course of herpes zoster infection in 42 otherwise normal hosts was studied by virus isolation and antibody titration. Varicella-zoster virus (VZV) was isolated from vesicle fluid from all three patients examined on the first day of the vesicular eruption and from five out of six examined on the second day. The isolation rate fell to one out of six patients on the seventh day of illness and VZV was not isolated from patients at a later stage of the illness. IgG antibodies were detected by IFAMA and ELISA, in sera from all the patients by the end of the first week of illness; IgG antibody titres were highest during the second and the third weeks. IgM antibodies to VZV were detected in sera from six of the 42 patients with herpes zoster after fractionation by ion-exchange chromatography.

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Enzyme-Linked Immunosorbent Assay for Detection of Virus-Specific IgM Antibodies to Varicella-Zoster Virus

Cited by 24 publications

References 11 publications

Detection of Specific IgA Antibodies in Serum of Kidney Transplant Patients with Recurrent Cytomegalovirus Infection

Detection of Specific IgA Antibodies in Serum of Kidney Transplant Patients with Recurrent Cytomegalovirus Infection

Antibody-capture enzyme-linked immunosorbent assays that use enzyme-labelled antigen for detection of virus-specific immunoglobulin M, A and G in patients with varicella or herpes zoster

Virological course of herpes zoster in otherwise normal hosts

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