Antibody to Multiple Mucoid Strains of Pseudomonas aeruginosa in Patients with Cystic Fibrosis, Measured by an Enzyme-Linked Immunosorbent Assay

Mucosal and systemic antibody responses to potentialPseudomonas aeruginosavaccine protein antigens in young children with cystic fibrosis following colonization and infection

“…A nested exploratory study was undertaken using material (BAL and sera) obtained as part of a prospective, longitudinal study of early CF lung disease in children in the state of Victoria, Australia. 27,42,48 The Royal Children's Hospital Ethics Committee approved this study and written informed consent was obtained from the parents or guardians of each child before undergoing flexible bronchoscopy and BAL.…”

“…Each were participants in the major birth cohort study. 27,42,48 Using methods described previously, paired serum and BAL fluid samples were obtained when the child was in a stable clinical state shortly after diagnosis and then annually until at least two to three years of fractions removed according to the method described by Suzuki and Terada. 53 All analytical SDS-PAGE was performed using the MinPROTEAN ® II Electrophoresis Unit (BioRad) according to the manufacturer's instructions.…”

“…[18][19][20] Several P. aeruginosa antigens invoke the characteristic rise in antibody titers as the disease state progresses and can be detected in the sera, sputa, saliva, tears and bronchoalveolar lavage (BAL) fluid from CF patients. [21][22][23][24][25][26][27] Specific antibody responses to various P. aeruginosa antigens have been studied in the sera of adult patients, however, the characterization of antibody responses in children who differ in their pulmonary clinical status during the early years of life and initial stages of infection has not been conducted. [28][29][30] A study investigating serum antibodies against alkaline phosphatase, elastase and exotoxin A in 183 CF patients (mean age 16.7 y) indicated that regular determination of serum antibody may be a useful indicative measure of probable infection for CF patients with negative or intermittent P. aeruginosa cultures.…”

Purification, characterization, and immunological cross-reactivity of alginates produced by mucoid Pseudomonas aeruginosa from patients with cystic fibrosis

“…The mucoid exopolysaccharide is chemically similar to the alginate from brown seaweed except that P. aeruginosa alginate is O acetylated (9,15,36). In previous studies of alginate from P. aeruginosa, a limited number of strains have been used as the source of alginate, and the extraction and purification procedures have differed widely resulting in varying degrees of purity of the final preparations (3,5,46,55,57).…”

Characterization of Antibody Response to Pseudomonas aeruginosa in Patients with Wound Infections

“…Pseudomonas aeruginosa is a common clinical isolate (1,3,5,(20)(21)(22)25). The progress from colonization to infection is usually accompanied by an increase of serum antibody titers to the main virulence factors of this microorganism-such as lipopolysaccharide (LPS) and exotoxin A (ExA) (6,22).…”