Antibody to Intrinsic Factor in Serum From Patients With Pernicious Anemia*

Studies of parietal cell antibody in pernicious anemia.

“…Sera from patients with pernicious anemia may also contain antibodies that react with gastric intrinsic factor (3)(4)(5)(6). This antibody has not been detected in gastric juice (20) 2) Parietal cell antibody was present in the ,yG-globulin fraction of serum; it was either absent or present in very low titers in the yA-globulin fraction, and it was not detected in the yM-globulin fraction.…”

“…It has been suggested, however, on the basis of indirect evidence, that the mucosal glands in the body and fundus of the stomach may be destroyed by an autoimmune process (2). Antibodies to gastric intrinsic factor (3)(4)(5)(6) and to gastric parietal cell cytoplasmic antigen (2,(7)(8)(9)) may be present in sera from patients with pernicious anemia. Furthermore, treatment of pernicious anemia patients with prednisolone may result in a regeneration of chief and parietal cells in the gastric mucosa, with secretion of acid and intrinsic factor and improvement in vitamin B12 absorption (10,11).…”

Radioiodination of human intrinsic factor

“…established by the presence of histamine-fast achlorhydria and abnormal vitamin Bi2 absorption which was corrected by human IF (9). Each serum was tested for antibody I or "blocking" activity by the charcoal test of Gottlieb, Lau, Wasserman, and Herbert (7) and for antibody II or "binding" activity by the antiglobulin coprecipitation technique of Taylor, Roitt, Doniach, Couchman, and Shapland (10) as well as by the electrophoretic retention test of Jeffries, Hoskins, and Sleisenger (11). P.A.…”

“…Reactions between "25I-IF-'CoB,2 and AbII were demonstrated by three techniques: (a) a modification of the antiglobulin coprecipitation technique of Taylor et al described in detail by Schade, Abels, and Schilling (12), (b) the Sephadex G200 gel filtration method of Imrie and Schilling (13), and (c) the electrophoretic retention test of Jeffries et al (11). Reactions between '"I-IF-°CoB&2 and AbI were demonstrated by incubating the '5I-IF-°CoB12 for 24 hr at 37'C with AbI to allow the AbI to exchange (14) with the 6'CoB12 bound to '25I-labeled IF.…”

Studies on Human Gastric Intrinsic Factor

“…However, the homogeneity of IF preparation could not be demonstrated in the ex periments using the Ouchterlony technique (2). Many investigations showed that immunodiffusion and immunoelectrophoresis combined with autoradiography were useful for testing human and hog IF (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). In the present investigation, the purified IF materials, obtained from normal human gastric juice (NHGJ) as described in the previous paper (13), were further analysed by immunodiffusion and immuno electrophoresis techniques combined with autoradiography, using the rabbit antisera obtained after successive injections of the purified materials with Freund's complete adjuvant in order to evaluate their purities.…”