Antibody responses to Four Corners hantavirus infections in the deer mouse (Peromyscus maniculatus): identification of an immunodominant region of the viral nucleocapsid protein

Abstract

Antibody responses to Four Corners hantavirus (FCV) infections in the deer mouse (Peromyscus maniculatus) were characterized by using FCV nucleocapsid protein (N), glycoprotein 1 (G1), and glycoprotein 2 (G2) recombinant polypeptides in Western immunoblot assays. Strong immunoglobulin G reactivities to FCV N were observed among FCV-infected wild P. maniculatus mice (n ‫؍‬ 34) and in laboratory-infected P. maniculatus mice (n ‫؍‬ 11). No immunoglobulin G antibody reactivities to FCV G1 or G2 linear determinants were detected. The strongest N responses were mapped to an amino-proximal segment between amino acids 17 and 59 (QLVTARQKLKDAERAVELDPDDVNKSTLQSRRAAVSALETKLG). FCV N antibodies cross-reacted with recombinant N proteins encoded by Puumala, Seoul, and Hantaan viruses.

Rapid and specific detection of Sin Nombre virus antibodies in patients with hantavirus pulmonary syndrome by a strip immunoblot assay suitable for field diagnosis

“…the 50-kDa Escherichia coli maltose binding protein (MBP) in the vector pMal C2 (Novagen, Madison, Wis.). The 100-kDa MBP-SNV N antigen was affinity purified by column chromatography on amylose resin and elution with maltose, according to the manufacturer's instructions (48). As with all of the recombinant antigens, purity was judged visually after overloading on a sodium dodecyl sulfate-polyacrylamide gel and staining the gel with Coomassie brilliant blue ( Fig.…”

Recent Advances in Hantavirus Molecular Biology and Disease

“…Hantaviral N protein has a molecular mass of approximately 50 kDa and contains 429-433 amino acid residues (de Carvalho Nicacio et al, 2001;Plyusnin et al, 1997;Van Epps et al, 1999). It is highly conserved across hantaviruses and contains cross-reactive epitopes that encompass the first 100 amino acids at N-terminal (Elgh et al, 1996;Gott et al, 1997;Schmaljohn et al, 1986Schmaljohn et al, , 1987Yamada et al, 1995). Further, serotype-specific conformational epitopes have been detected in about half of the C-termini of N proteins by serotype-specific monoclonal antibodies (MAbs) (Ruo et al, 1991;Yoshimatsu et al, 1996).…”

Molecular linkage of hantavirus pulmonary syndrome to the white-footed mouse, Peromyscus leucopus: genetic characterization of the M genome of New York virus

“…Since antibody titers against PUU were low, titration studies used only PH-and SEO-infected cells. Additional supplementary antibody testing was done by Western blot (immunoblot) analysis with the nucleocapsid antigen of FC expressed as a TrpE fusion protein in Escherichia coli as previously described (19,42).…”