Antibody Responses to Antigens of Streptococcus mutans in Monkeys (Macaca fascicularis) Immunized against Dental Caries

Russell, R. R. B.; Peach, Susan; Colman, G.; Cohen, Bernard

doi:10.1099/00221287-129-3-865

Cited by 23 publications

(29 citation statements)

References 6 publications

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“…More recently, low molecular weight peptides have been generated from this antigen that confer protec tion without any possibility of side-effects [Lehner et al, 1985a]. Protection in primates varies with the immunogen, the route of immunization, and whether the animals have been exposed to fluoride, but reductions in caries have ranged from 50-60% [Lehner et al, 1981] to >90% [Lehn er et al, 1985a]; in one study, complete protection was achieved for 3 years [Russell et al, 1983]. There have been no clinical trials in humans of an anticaries vaccine.…”

Section: Vaccinationmentioning

confidence: 99%

Antimicrobial Strategies in the Prevention of Dental Caries

Marsh¹

1993

Caries Res

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Antimicrobial agents, applied either professionally or delivered from dentifrices or mouthwashes, could reduce caries by controlling plaque formation, suppressing cariogenic species, or by inhibiting bacterial metabolism. Chlorhexidine has proven anticaries activity; other agents might also prevent caries by means of their antiplaque properties. Sugar substitutes stabilize the microflora by reducing the number of acid challenges to plaque and stimulating saliva flow; some also have antimicrobial properties, especially against mutans streptococci. Vaccines have been prepared from purified antigens of mutans streptococci. These vaccines confer protection in non-human primates, but have yet to be tested in a human clinical trial.

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Section: Vaccinationmentioning

confidence: 99%

Antimicrobial Strategies in the Prevention of Dental Caries

Marsh¹

1993

Caries Res

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“…Two groups in England have independently demon strated the presence of specific proteins designated antigen I/II, I, II and III [Rus sell and Lehner, 1978;Russell et al, 1980a, b] and antigens A, B, C and D [Russell, 1979a;Russell et al, 1983] in cell extracts and culture supernatants of S. mutans se rotype c. Antigens I/II, I and B are high molecular weight proteins with antigen I/II existing as a combined form of anti gen I and II [Russell et al, 1980a], Other high mulecular weight proteins have later been identified and named IF [Hughes et al, 1980], PI [Forester et al, 1983] and SpaA [Holt et al, 1982], Antigen I/II seems to be identical with antigen B and PI [Forester et al, 1983] and with SpaA [Holt et al, 1982] and antigen B is iden tical with antigen IF [Russell, 1979a], Elec tron microscopic studies have recently shown that antigens I/II and III are pres ent on the outer surface of the cell wall structure [Moro and Russell, 1983] and these proteins can therefore be regarded as surface antigens. Protective immunity to dental caries has been demonstrated in monkeys immunized with antigens 1/11 and 1 [Lehner et al, 1980 and with antigens A and C .…”

Section: Candidate Antigensmentioning

confidence: 99%

An Anticaries Vaccine: Report on the Status of Research

Krasse¹,

Emilson²,

Gahnberg³

1987

Caries Res

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The state of the art of the research on a vaccine against dental caries has been reviewed. The subject has been analysed both from the biological and from the public health point of view. It is concluded that Streptococcus mutans plays a key role for the development of dental caries and that a vaccine directed against this microorganism could be a valuable adjunct to existing preventive measures in some countries. In others it could be a major public health measure. The unique characteristics of dental caries partly explain why a vaccine is not yet available. Protection against dental caries by immunization could be achieved by protective antibodies from serum, by IgA antibodies in saliva or by a combined effect of serum and salivary components. In rodents both peroral and parenteral immunization has provided protection against S. mutans infection and dental caries. In primates only parenteral immunization has given positive results. In humans peroral immunization has led to an increased salivary antibody activity in some studies but not in others. There is no conclusive evidence which shows that antibodies against S. mutans can influence dental caries activity in man. As structures on the cell surface of S. mutans cross-react with human tissue much work has been concentrated on identification of candidate antigens. Some of these have been purified and tested in vitro and in animal systems. Some protein antigens have given a clear-cut caries protection in primates and one of these has been produced in large amounts and is ready for testing in humans. Another candidate antigen, the enzyme complex called glucosyltransferase has shown protection in rodents and the effect of peroral administration of this antigen is now being tested in man. A considerable amount of knowledge has been obtained and the activity in the research area is high. Some groups have focused on basic immunology and have little understanding of the disease. In other groups the reverse is true. A consequence of this is that collaborative studies ought to be organized. By such studies key information about the possible role of antibodies in the development of dental caries in man could be obtained within a reasonable period of time.

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“…Samples of the lysates were then examined for their antigen content by rocket immunoelectrophoresis using rabbit antisera specific for S . mutans wall-associated protein antigens A, B and C (Russell et al, 1983b), GTF and GBP (Russell, 1979a;Russell et al, 1983~). Four of the lysates gave precipitin peaks with the antiserum to GBP.…”

Section: R E S U L T Smentioning

confidence: 99%

Expression of a Gene for Glucan-binding Protein from Streptococcus mutans in Escherichia coli

1985

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The structural gene for a glucan-binding protein (GBP) of Streptococcus mutans has been inserted into a bacteriophage A vector and expressed in Escherichia coli K12. Lysates of E. coli infected with the recombinant phage contain an antigenic protein of the same size as S . mutans GBP. The GBP synthesized in E. coli can be affinity-purified on immobilized glucan and antiserum raised against it has been shown to precipitate fructosyltransferase activity from S . mutans.

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Antibody Responses to Antigens of Streptococcus mutans in Monkeys (Macaca fascicularis) Immunized against Dental Caries

Cited by 23 publications

References 6 publications

Antimicrobial Strategies in the Prevention of Dental Caries

Antimicrobial Strategies in the Prevention of Dental Caries

An Anticaries Vaccine: Report on the Status of Research

Expression of a Gene for Glucan-binding Protein from Streptococcus mutans in Escherichia coli

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