Expression of a Gene for Glucan-binding Protein from Streptococcus mutans in Escherichia coli

Russell, R. R. B.; Coleman, David C.; Dougan, Gordon

doi:10.1099/00221287-131-2-295

Cited by 52 publications

(53 citation statements)

References 12 publications

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“…GbpA was originally isolated by Russell (1979) and designated GBP (Douglas and Russell, 1982;Russell et al, 1985). Several years later, Smith et al (1994) reported the isolation of GbpB.…”

Section: S Mutans Gbpsmentioning

confidence: 99%

Glucan-binding Proteins of the Oral Streptococci

Banas

Vickerman

2003

Critical Reviews in Oral Biology & Medicine

291

262

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ABSTRACT:The synthesis of extracellular glucan is an integral component of the sucrose-dependent colonization of tooth surfaces by species of the mutans streptococci. In investigators' attempts to understand the mechanisms of plaque biofilm development, several glucan-binding proteins (GBPs) have been discovered. Some of these, the glucosyltransferases, catalyze the synthesis of glucan, whereas others, designated only as glucan-binding proteins, have affinities for different forms of glucan and contribute to aspects of the biology of their host organisms. The functions of these latter glucan-binding proteins include dextran-dependent aggregation, dextranase inhibition, plaque cohesion, and perhaps cell wall synthesis. In some instances, their glucan-binding domains share common features, whereas in others the mechanism for glucan binding remains unknown. Recent studies indicate that at least some of the glucan-binding proteins modulate virulence and some can act as protective immunogens within animal models. Overall, the multiplicity of GBPs and their aforementioned properties are testimonies to their importance. Future studies will greatly advance the understanding of the distribution, function, and regulation of the GBPs and place into perspective the facets of their contributions to the biology of the oral streptococci.

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“…GbpA was originally isolated by Russell (1979) and designated GBP (Douglas and Russell, 1982;Russell et al, 1985). Several years later, Smith et al (1994) reported the isolation of GbpB.…”

Section: S Mutans Gbpsmentioning

confidence: 99%

Glucan-binding Proteins of the Oral Streptococci

Banas

Vickerman

2003

Critical Reviews in Oral Biology & Medicine

291

262

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“…Streptococcus mutans GS-5, serotype c (Bratthall, 1970) and transposon-generated auxotrophs of S. niutans GS-5 (Procino et al, 1988) were grown in Todd-Hewitt broth (Difco) Smorawinska et al (1983); gbp, Russell et al (1985); gtfA, Robeson et al (1983); gtfB, Aoki et al (1986); g t f c , Hanada & Kuramitsu (1988); gtfD, Hanada & Kuramitsu (1989); ldh, Hillman et al (1990); pac, Lee et al (1988), Okahashi et al (1989);scrA, Sat0 et al (1989); scrB, Hayakawa et al (1986), Lunsford & Macrina, (1986; wapA, Ferretti et al (1989). DNA isolation.…”

Section: Methodsmentioning

confidence: 99%

Chromosome organization of Streptococcus mutans GS-5

Hantman

Sun²,

Piggot³

et al. 1993

Journal of General Microbiology

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Twenty-eight genetic loci have been physically mapped to specific large restriction fragments of the Streptococcus mutans GS-5 chromosome by hybridization with probes of cloned genes or, for transposon-generated amino acid auxotrophs, with probes for Tn916. In addition, restriction fragments generated by one low-frequency-cleavage enzyme were used as probes to identify overlapping fragments generated by other restriction enzymes. The approach allowed construction of a low resolution physical map of the S. mutans GS-5 genome using restriction enzymes ApaI (5'-GGGCC/C), SmaI (5'-CCC/GGG), and NotI (5'-GC/GGCCGC).

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“…All tests were performed in triplicate. To confirm the data obtained from the RPLA assays, 50% of the septicaemia and nasal isolates were tested for production of enterotoxins A, B, C, and D by Western immunoblotting (Burnette, 1981) with specific rabbit anti-enterotoxin sera (Russell et al, 1985) and 50-fold concentrated culture supernate proteins, as described by Coleman et al (1986). In a similar fashion, all isolates which were TSST-1-positive (TSST-1 +), as determined by RPLA assays, were re-tested for TSST-1 production by immunoblotting with specific rabbit anti-TSST-1 serum.…”

Section: Detection Of S Aureus Enterotoxins and Toxic Shock Syndrommentioning

confidence: 99%