Antibody responses against <i>Porphyromonas gingivalis</i> infection in patients with early‐onset periodontitis

Guo, Shujuan; Takahashi, Keiso; Kokeguchi, Susumu; Takashiba, Shogo; Kinane, Denis F.; Murayama, Yoji

doi:10.1034/j.1600-051x.2000.027010769.x

Cited by 25 publications

(24 citation statements)

References 42 publications

(51 reference statements)

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“…The quantity of antibody has been related to the severity/extent of disease, exacerbations of disease progression, and therapeutic interventions 28,35‐37 . The bacterial specificity of the elevated antibody varies among patients and categories of periodontitis 29,38‐43 . Some recent studies 24,44,45 suggested that the level and isotype of antibody in maternal serum or cord blood, with specificity for individual oral species, is correlated with an increased risk for PTB/LBW deliveries.…”

Section: Discussionmentioning

confidence: 99%

Systemic Immune Responses in Pregnancy and Periodontitis: Relationship to Pregnancy Outcomes in the Obstetrics and Periodontal Therapy (OPT) Study

Ebersole

Novak

Michalowicz

et al. 2009

Journal of Periodontology

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Background Our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the Obstetrics and Periodontal Therapy (OPT) Study. This article describes the systemic antibody responses to selected periodontal bacteria in the same patients. Methods Serum samples, obtained from pregnant women at baseline (13 to 16 weeks; 6 days of gestation) and 29 to 32 weeks, were analyzed by enzyme-linked immunosorbent assay for serum immunoglobulin G (IgG) antibody to Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Campylobacter rectus, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia (previously T. forsythensis), and Treponema denticola. Results At baseline, women who delivered live preterm infants had significantly lower total serum levels of IgG antibody to the panel of periodontal pathogens (P = 0.0018), to P. gingivalis (P = 0.0013), and to F. nucleatum (P = 0.0200) than women who delivered at term. These differences were not significant at 29 to 32 weeks. Changes in IgG levels between baseline and 29 to 32 weeks were not associated with preterm birth when adjusted for treatment group, clinical center, race, or age. In addition, delivery of low birth weight infants was not associated with levels of antibody at baseline or with antibody changes during pregnancy. Conclusions Live preterm birth is associated with decreased levels of IgG antibody to periodontal pathogens in women with periodontitis when assessed during the second trimester. Changes in IgG antibody during pregnancy are not associated with birth outcomes.

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Section: Discussionmentioning

confidence: 99%

Systemic Immune Responses in Pregnancy and Periodontitis: Relationship to Pregnancy Outcomes in the Obstetrics and Periodontal Therapy (OPT) Study

Ebersole

Novak

Michalowicz

et al. 2009

Journal of Periodontology

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“…Proteins were extracted by the method of Guo et al (2000). Proteins were extracted by the method of Guo et al (2000).…”

Section: Western Immunoblottingmentioning

confidence: 99%

The potential role of interleukin‐17 in the immunopathology of periodontal disease

Takahashi

Azuma

Motohira

et al. 2005

J Clinic Periodontology

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173

169

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“…In the present series, however, no specific clinicopathological features characterizing Ag53-specific antibody response were recognized. Kurihara et al (1991) and Guo et al (2000) documented the Western blot detection of antibodies against Ag53 in the serum of 70% of periodontitis patients. In the present study, the antibodies to Ag53 were not detected in the serum with the AlphaScreen method.…”

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confidence: 99%

In situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: The application of the enzyme-labeled antigen method

et al. 2014

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SUMMARYPorphyromonas gingivalis is a keystone periodontal pathogen. Histologocally, the gingival tissue in periodontitis shows dense infiltration of plasma cells. However, antigens recognized by antibodies secreted from the immunocytes remain unknown. The enzyme-labeled antigen method was applied to detecting plasma cells producing P. gingivalisspecific antibodies in biopsied gingival tissue of periodontitis. N-terminally biotinylated P. gingivalis antigens, Ag53 and four gingipain domains (Arg-pro, Arg-hgp, Lys-pro and Lys-hgp) were prepared by the cell-free protein synthesis system using wheatgerm extract. With these five labeled proteins as probes, 20 lesions of periodontitis were evaluated. With the AlphaScreen method, antibodies against any one of the five P. gingivalis antigens were detected in 11 (55%) serum samples and 17 (85%) tissue extracts. Using the enzymelabeled antigen method on paraformaldehyde-fixed frozen sections of gingival tissue, plasma cells were labeled with any one of the five antigens in 17 (94%) of 18 specimens, in which evaluable plasma cells were detected. The positivity rates in periodontitis were significantly higher than those found previously in radicular cysts (20% in sera and 33% in tissue extracts with the AlphaScreen method, and 25% with the enzyme-labeled antigen method). Our findings directly indicate that antibodies reactive to P. gingivalis are locally produced in the gingival lesions, and that inflammatory reactions against P. gingivalis are involved in periodontitis.

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Antibody responses against Porphyromonas gingivalis infection in patients with early‐onset periodontitis

Cited by 25 publications

References 42 publications

Systemic Immune Responses in Pregnancy and Periodontitis: Relationship to Pregnancy Outcomes in the Obstetrics and Periodontal Therapy (OPT) Study

Systemic Immune Responses in Pregnancy and Periodontitis: Relationship to Pregnancy Outcomes in the Obstetrics and Periodontal Therapy (OPT) Study

The potential role of interleukin‐17 in the immunopathology of periodontal disease

In situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: The application of the enzyme-labeled antigen method

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