Abstract:Summary
Female guinea pigs, inoculated intravaginally with a herpes simplex virus‐type 2 (HSV‐2) strain M1, developed typical symptoms of a primary and recurrent genital herpes infection. Sera from HSV‐2 infected guinea pigs taken during the primary or recurrent stages of the genital infection contained complement (C)‐fixing antibody which reacted with an apparent type specificity to an early 4 h HSV‐2 infected cell extract (AG‐4) when compared to a 4 h HSV‐1 infected cell extract. This C‐fixing anti‐AG‐4 acti… Show more
“…Evidence of neutralizing antibody activity was considered when BHK-21 cells exhibited less than 30 % cytopathic effect. For antibody characterization, 18-hr BHMV CSIRO 290 (late) infected BHK-21 cell extracts were used in a urease-ELISA assay as described previously (5). Guinea pig sera were also used to immunoprecipitate 35S-methionine-labeled BHMV CSIRO 290 and HSV-2M1 late proteins as described previously (5).…”
“…Evidence of neutralizing antibody activity was considered when BHK-21 cells exhibited less than 30 % cytopathic effect. For antibody characterization, 18-hr BHMV CSIRO 290 (late) infected BHK-21 cell extracts were used in a urease-ELISA assay as described previously (5). Guinea pig sera were also used to immunoprecipitate 35S-methionine-labeled BHMV CSIRO 290 and HSV-2M1 late proteins as described previously (5).…”
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