Antibody response of immunodeficient (xid) CBA/N mice to Escherichia coli 0113 lipopolysaccharide, a thymus-independent antigen.

Hiernaux, Jean; Jones, Janet M.; Rudbach, Jon J.A.; Rollwagen, Florence M.; Baker, Phillip P.J.

doi:10.1084/jem.157.4.1197

Cited by 16 publications

(12 citation statements)

References 34 publications

(37 reference statements)

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“…(With respect to the lat ter, the situation is actually more complicated -see the Discussion.) In contrast to this, in two different CRI systems [10,11] no impairment in the relative production of CRI+ Abs in xid mice was found. In the anti-p-azophenylarsonate (Ar)-CRI system in A strain mice, two apparently contradictory reports have appeared.…”

Section: Introductionmentioning

confidence: 48%

Influence of xid on Anti-Azophenylarsonate (Ar) Antibody Responses of (CBA/N × A/J)F1 Mice: Differential Idotype Expression Induced by Only One of Two Ar Antigens

Conger¹

1987

Int Arch Allergy Immunol

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The influence of an X chromosome-linked immune deficiency gene (xid) on several properties of the anti-azophenylarsonate (Ar) antibody responses of (CBA/N × A/J)F₁ (NAF₁) mice was examined. With respect to response magnitude, it was found that male, xid-expressing NAF₁ mice showed about 1/3 the concentration of serum anti-Ar antibody as normal female NAF₁ mice in hyperimmune responses to Brucella abortus (BA)-Ar. In responses induced by keyhole limpet hemoycanin (KLH)-Ar, males showed responses of about 1/8 to 1/2 the female levels, depending on the assay time point. The kinetics of the latter response were identical in mice of the two sexes. No significant difference could be detected in the time-dependent avidity maturation of the anti-Ar antibody elicited by KLH-Ar in xid vs. normal mice. The isotype profile of the day 38 anti-Ar primary response elicited by KLH-Ar in male NAFi mice differed from that of the female mice in two ways: (1) IgG_2a levels were depressed, and (2) a significantly lower number of the male mice demonstrated detectable IgG₃ anti-Ar antibody production. The primary focus of this work was to determine the effect of xid on the expression of the major cross-reactive idiotype – CRI_A – of A strain mice. It was found that while a significantly higher proportion of the female mice could be classified as high CRU producers in responses to BA-Ar, no difference could be demonstrated if the inducing antigen was KLH-Ar. It is proposed that the difference observed with the two antigens may be due to the more selective activation by KLH-Ar of a small subset of high affinity Ar-specific clones – which may be enriched for CRI_A + precursors – in both normal and immune defective mice. In contrast, BA-Ar may ‘sample’ more of the total anti-Ar repertoire and thus reveal within it an xid-determined depression in the proportion of CRI_A + clones. Finally, it is noted that the influence of xid appears to be largely of a stochastic, and not an absolute character.

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Section: Introductionmentioning

confidence: 48%

Influence of xid on Anti-Azophenylarsonate (Ar) Antibody Responses of (CBA/N × A/J)F1 Mice: Differential Idotype Expression Induced by Only One of Two Ar Antigens

Conger¹

1987

Int Arch Allergy Immunol

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“…As expected, LPSsensitive C3H/HeN-MTV+ and LPS-resistant C3H/HeJ mice differed greatly (almost eightfold; P < 0.05) in their capacity to make an antibody response to LPS 0113. RIIIS/J mice usually make a low response to this antigen (Table 3, 22). However, when these strains of mice were tested for their capacity to make an antibody response to SSS-III, RIIIS/J mice gave a low antibody response (Table 3; 22), whereas C3H/HeN-MTV+ and C3H/HeJ mice made relatively high antibody responses of the same magnitude (P > 0.05).…”

Section: Methodsmentioning

confidence: 99%

“…Although the antibody response to LPS is directed mainly against the polysaccharide portion of the molecule (21, 32), the genetic defect of C3H/HeJ mice does not influence their capacity to elicit an antibody response to all polysaccharide antigens; these mice make an excellent antibody response to SSS-III (Table 3). CBA/N mice lack an X-linked gene having a decisive (an almost all-or-none) influence on the capacity to make an IgM antibody response to several, but not all, helper T-cell-independent bacterial polysaccharide antigens (2,3,21,28,29). This genetic defect, which is associated with abnormally low levels of IgM in serum (2), is thought to involve either the absence of a particular subset of B cells (for a review, see references 28 and 29) or the failure of B Tables 1 and 4. b Fractional number of (RB)F2 mice giving a 5-day PFC response to 20 1Lg of LPS 0113, 0.5 p.g of SSS-III, or 100 jig of dextran B-1355 that was within the range values (PFC per spleen) for the RIIIS/J phenotype.…”

Section: Methodsmentioning

confidence: 99%

Influence of multiple genes on the magnitude of the antibody response to bacterial polysaccharide antigens

Baker¹,

Rudbach²,

Prescott³

et al. 1984

Infect Immun

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Studies conducted with Fl and F2 progeny of crosses between strains of inbred mice that differ greatly in their capacity to make an antibody response to type III pneumococcal polysaccharide, dextran B-1355, and lipopolysaccharide from Escherichia coli 0113 have shown that multiple genes influence the magnitude of the antibody response to these antigens. Other studies with hybrids derived from crosses between C3H/HeJ, CBA/ N, and RIIIS/J mice have indicated that the genetic defects characteristic of these strains of mice are dissimilar and unlinked and that autosomal, as well as X-linked, genes control serum immunoglobulin M in unimmunized mice.

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“…Intraperitoneal injection of A/10/25, a prototype vaccine strain suitable for animal experiments, elicited an immune response characterized by increases in the levels of IgM and IgG antibodies. It has long been accepted that lipopolysaccharide is a potent antigen and that the polysaccharide moiety bears the major determinants to which the immune system reacts (6,13). Antibodies raised to killed P. aeruginosa are mainly directed to the O-polysaccharide fractions and the semirough fractions of the lipopolysaccharide, with some activity against several proteins.…”

mentioning

confidence: 99%

Enzyme-linked immunosorbent assay antibody responses to a temperature-sensitive mutant of Pseudomonas aeruginosa

Sordelli¹,

Rojas²,

Cerquetti³

et al. 1985

Infect Immun

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The serum immunoglobulin G and M responses induced by immunization of mice with teftiperature-sensitive mutant A/10/25 of Pseudomonas aeruginosa were evaluated by enzyme-linked immunosorbent assay. These antibody responses were immunotype specific, and the immunoglobulin G antibody level, although low, was still significant by day 52 after vaccination. NOTES 325on August 5, 2020 by guest http://iai.asm.org/ Downloaded from

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Antibody response of immunodeficient (xid) CBA/N mice to Escherichia coli 0113 lipopolysaccharide, a thymus-independent antigen.

Cited by 16 publications

References 34 publications

Influence of <i>xid</i> on Anti-Azophenylarsonate (Ar) Antibody Responses of (CBA/N × A/J)F<sub>1</sub> Mice: Differential Idotype Expression Induced by Only One of Two Ar Antigens

Influence of <i>xid</i> on Anti-Azophenylarsonate (Ar) Antibody Responses of (CBA/N × A/J)F<sub>1</sub> Mice: Differential Idotype Expression Induced by Only One of Two Ar Antigens

Influence of multiple genes on the magnitude of the antibody response to bacterial polysaccharide antigens

Enzyme-linked immunosorbent assay antibody responses to a temperature-sensitive mutant of Pseudomonas aeruginosa

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