Antibody Response of Adults to an Aluminum Hydroxide-Adsorbed Neisseria meningitidis Serotype 2b Protein-Group B Polysaccharide Vaccine

Abstract

A group B Neisseria meningitidis serotype protein vaccine was studied clinically in adults. The vaccine comprised lipopolysaccharide-depleted outer membrane vesicles from a serotype 2b strain, 3006-M2, noncovalently complexed with group B meningococcal polysaccharide. Volunteers received 25 micrograms each of protein and polysaccharide administered intramuscularly either in 0.9% NaCl or adsorbed onto aluminum hydroxide on weeks 0 and 6. Most individuals experienced mild local reactions, but there were no systemic reactions. Both vaccine formulations stimulated antibodies to the outer membrane proteins of serotypes 2a:P1.2 and 2b:P1.2, but higher levels were achieved with the aluminum hydroxide-adsorbed vaccine after two immunizations. Vaccine-induced antibodies were primarily IgG and were bactericidal for both a serotype 2a and a serotype 2b strain. Induction of bactericidal antibodies has been shown to be a major predictor of protection against meningococcal disease.

Estimation of Lactose Interference in Vaccines and a Proposal of Methodological Adjustment of Total Protein Determination by the Lowry Method

“…1C and 1D. These results indicated that the blue color resulting from lactose was similar to that resulting from BSA, and thus confirmed that lactose interferes with the assay, as previously described by Frasch et al (14). In addition, these results revealed that lactose in samples should be removed in order to accurately determine the protein content.…”

“…Lactose has been previously reported to interfere with the Lowry assay of a vaccine (14), but the degree to which lactose interfered with the Lowry assay was unclear. In this study, it was demonstrated that AE0.1 mg/mL lactose caused interference.…”

“…In fact, it has been reported that lactose in the aluminum hydroxideadsorbed Neisseria meningitidis serotype 2b proteingroup B polysaccharide vaccine interferes with the Lowry assays (14). The vaccine's protein content was assessed by a modified Lowry method using a TCA precipitation step and, although the TCA precipitation was probably useful for avoiding lactose interference, detailed information was not provided (14).…”

Characterization of a stress protein from group B Neisseria meningitidis

“…OMV were prepared by concentrating the culture supernatant of meningococci grown for 60 h to examine whether Msp65 was localized in the outer membrane (9 (10). From the protein elution and ELISA activity profiles (Fig.…”

Imunidade conferida por vacinas anti-meningocócicas

“…Da mesma forma, o polissacáride B purificado e de alto peso molecular induz primariamente à produção de IgM 19,31 , mesmo quando conjugado a proteínas carreadoras e adsorvidos ao hidróxido de alumínio 19,35 . Esta pobre imunogenicidade é atribuída, principalmente, à semelhança antigênica do polissacáride B com glicolípides e glicoproteínas presentes em células humanas, à sensibilidade a neuraminidases do organismo humano e a instabilidade tridimensional da molécula 31 .…”