Antibody-Mediated Hemadsorption by Tumor Tissues

Anti-γ-GIobulin Factors in Serum and Tissue of Cancer Patients

“…A similar finding was previously made in studying sections of methylcholanthrene-induced sarcomas of mice [20], however, in the studies on murine sarcomas sheep erythrocytes sensitized by rabbit anti-sheep erythrocyte scrum were mostly used. The authors termed this phenom enon 'antibody-mediated hemadsorption' and found that it was mostly if not exclusively due to the reaction between the tumor tissue and the Fc fragment of the antibody sensitizing the red blood cells.…”

Studies on the Fc receptor bearing cells in a transplanted methylcholanthrene induced mouse fibrosarcoma

“…It was inactivated at 56°C for 30 min before use in the rosette assays. Studies undertaken with Sephadex G-200 fractions of this serum indicated that the maximum number of rosettes was obtained with SRBC sensitized with the 7S fraction (see also Milgrom et al, 1968). A series of preliminary experiments was also performed to ascertain the optimum conditions for the rosette forming assay.…”

“…A number of observations also indicate that a significant proportion of the cells in certain nonlymphoreticular tumours may also possess Fc receptors (Milgrom et al, 1968;Cohen, Gurner and Coombs, 1971;Tonder, Morse and Humphrey, 1974;Kerbel and Davies, 1974). These Fc receptors have been demonstrated by both cryostat haemadsorption techniques (Milgrom et al, 1968;Tonder et al, 1974) and by conventional rosetting procedures with suspensions of tumour cells and antibody coated sheep erythrocytes (Cohen et al, 1971;Kerbel and Davies, 1974). The tumours studied included a variety of human adenocarcinomata and epidermoid carcinomata (Tonder et al, 1974), tumours of murine connective and epithelial tissue (Kerbel and Davies, 1974) and a transmissible venereal tumour in the dog (Cohen et al, 1971 (MacLennan, 1972).…”

“…DETAILED studies during the past few years have convincingly demonstrated that normal and malignant lymphoid and reticuloendothelial cells may have on their surface receptors for antigen/antibody complexes and aggregated JgG, the so-called Fc receptors (reviewed by Kerbel and Davies, 1974). A number of observations also indicate that a significant proportion of the cells in certain nonlymphoreticular tumours may also possess Fc receptors (Milgrom et al, 1968;Cohen, Gurner and Coombs, 1971;Tonder, Morse and Humphrey, 1974;Kerbel and Davies, 1974). These Fc receptors have been demonstrated by both cryostat haemadsorption techniques (Milgrom et al, 1968;Tonder et al, 1974) and by conventional rosetting procedures with suspensions of tumour cells and antibody coated sheep erythrocytes (Cohen et al, 1971; Kerbel and Davies, 1974).…”

“…These suspensions were prepared as described earlier. Both types of preparation gave rise to tumours in which there were cells expressing Fc receptors on their surface (see Table X (Milgrom et al, 1968;Cohen et al, 1971;T0nder et al, 1974;Kerbel and Davies, 1974 . Experiments are in progress to characterize the effector cell in this system and to establish if it is of host origin.…”