Antibody manufacture in transgenic animals and comparisons with other systems

Houdebine, Louis

doi:10.1016/s0958-1669(02)00362-2

Cited by 72 publications

(51 citation statements)

References 53 publications

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“…The efficiency of these techniques is continually improving since conditions of culture are better and better controlled. Cells produce higher and higher quantities of proteins and cells are being engineered to optimise glycosylation and other posttranslational modifications [32,45]. Yet, CHO cells have limited production capacity and the implementation of new CHO fermentors remains costly, slow and poorly flexible [45].…”

Section: Discussionmentioning

confidence: 99%

“…Whatever happens, it is important to note that about 500 monoclonal antibodies are under preclinical or clinical studies and that 50−100 other recombinant proteins should be produced in the coming years or decades (50). Some experts consider that the demand is increasing and that all the available systems might be required to meet the demand in one decade hence [32].…”

Section: Discussionmentioning

confidence: 99%

“…Milk is presently the most mature transgenic system for the production of recombinant pharmaceutical proteins [32]. About 100 proteins have been prepared in mouse milk experimentally.…”

Section: Production Of Pharmaceutical Proteins In Milkmentioning

confidence: 99%

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Preparation of recombinant proteins in milk to improve human and animal health

Soler¹,

Thépot²,

Rival‐Gervier³

et al. 2006

Reprod. Nutr. Dev.

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-Milk is a very abundant source of proteins for animal and human consumption. Milk composition can be modified using transgenesis, including exogenous gene addition and endogenous gene inactivation. The study of milk protein genes has provided researchers with regulatory regions capable of efficiently and specifically driving the expression of foreign genes in milk. The projects underway are aimed at modifying milk composition, improving its nutritional value, reducing mammary infections, providing consumers with antipathogen proteins and preparing purified recombinant proteins for pharmaceutical use. The present paper summarises the current progress in this field. milk / recombinant proteins / animal and human health

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of recombinant proteins in milk to improve human and animal health

Soler¹,

Thépot²,

Rival‐Gervier³

et al. 2006

Reprod. Nutr. Dev.

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are several potential advantages of using scFv fragments over whole antibodies or larger fragments for liposome targeting. These include: i) slower clearance than mAb-targeted liposomes, as Fc-mediated clearance is eliminated [55]; ii) theoretically lower production cost for scFv fragments generated from bacterial cultures relative to whole antibodies generated from animal ascites or cell culture [56,57]; iii) the ability to select scFv with the desired affinity and specificity using phage display [58]; iv) the option of engineering tags into scFv constructs, which can aid in their identification and purification [59]; and v) the ability to engineer fully human fragments or fragments with low levels of non-human content, which will reduce the risk of immunogenic reactions [60,61].…”

Section: Monoclonal Antibodies and Fragments Of Antibodies-wholementioning

confidence: 99%

The use of single chain Fv as targeting agents for immunoliposomes: an update on immunoliposomal drugs for cancer treatment

Cheng

Allen

2010

Expert Opinion on Drug Delivery

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Importance of the field-Targeted liposomal drugs represent the next evolution of liposomal drug delivery in cancer treatment. In various preclinical cancer models, antibody-targeted PEGylated liposomal drugs have demonstrated superior therapeutic effects over their non-targeted counterparts. Single chain Fv (scFv) has gained popularity in recent years as the targeting agent of choice over traditional targeting agents such as monoclonal antibodies (mAb) and antibody fragments (e.g., Fab′).Areas covered in this review-This review is focused mainly on advances in scFv-targeted liposomal drug delivery for the treatment of cancers, based on a survey of the recent literature, and on experiments done in a murine model of human B-lymphoma, using anti-CD19 targeted liposomes targeted with whole mAb, Fab′ fragments and scFv fragments.What the reader will gain-This review examines the recent advances in PEGylated immunoliposomal drug delivery, focusing on scFv fragments as targeting agents, in comparison with Fab′ and mAb.Take home message-For clinical development, scFv are potentially preferred targeting agents for PEGylated liposomes over mAb and Fab′, owing to factors such as decreased immunogenicity, and pharmacokinetics/biodistribution profiles that are similar to non-targeted PEGylated (Stealth ® ) liposomes.

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“…Many other proteins and mainly chimaeric; humanised or human monoclonal antibodies are expected to be produced at a moderate cost in the coming decade and later [35].…”

Section: The Applications and The Future Of Animal Transgenesismentioning

confidence: 99%

Relations between animal transgenesis and reproduction

Houdebiné¹

2005

Reprod. Nutr. Dev.

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-Transgenesis has become an essential tool for the study of gene expression mechanisms and functions. Transgenesis is also more and more used for biotechnological applications such as the study of human diseases, the adaptation of pig organs to humans, the production of pharmaceutical proteins in milk and likely in the future for the improvement of animal production. The use of transgenesis relies on the efficiency of gene transfer. New tools have been recently designed to improve gene transfer. The methods of gene transfer are highly dependent on the techniques of animal reproduction. Conversely, the need to improve transgenesis urges researchers to study some of the key steps in reproduction and to find new techniques for gene transfer. This paper summarises the recent data and the perspectives offered by animal transgenesis. transgenesis / animal production / biotechnological applications

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Antibody manufacture in transgenic animals and comparisons with other systems

Cited by 72 publications

References 53 publications

Preparation of recombinant proteins in milk to improve human and animal health

Preparation of recombinant proteins in milk to improve human and animal health

The use of single chain Fv as targeting agents for immunoliposomes: an update on immunoliposomal drugs for cancer treatment

Relations between animal transgenesis and reproduction

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