Antibody-ELISA for Trypanosoma evansi: Application in a serological survey of dairy cattle, Thailand, and validation of a locally produced antigen

Desquesnes, Marc; Kamyingkird, Ketsarin; Pruvot, Mathieu; Kengradomkij, Chanya; Bossard, Géraldine; Sarataphan, Nopporn; Jittapalapong, Sathaporn

doi:10.1016/j.prevetmed.2009.04.011

Cited by 38 publications

(41 citation statements)

References 26 publications

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“…In our study, we found a surprisingly low rate of Ab-ELISA positive tests, in comparison to CATT and PCR results. This discrepancy is likely due to a failure for this specific experiment to detect circulating IgG, since this test gave previously robust results (Desquesnes et al, 2009a, Desquesnes et al, 2009b, OIE, 2012. In this experiment, sera were frozen, thawed, then spotted and let 2 to 12 weeks on the spot before performing the ELISA.…”

Section: Discussionmentioning

confidence: 98%

“…With passive hemagglutination test and with Ag-ELISA (Antigene Enzyme Linked Immuno-Sorbent Assay) the prevalence was 19.7 and 13.8% respectively (Omer et al, 1998), but those tests are not the one recommended for T. evansi diagnostics by the World Organization for Animal Health (OIE, 2012). The use of a combination of convenient diagnosis tools is of high importance to get a clear epidemiological status of the camel flock in a given country (Desquesnes et al, 2009b), and thus propose efficient control methods.…”

Section: Introductionmentioning

confidence: 99%

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Epidemiological Surveys of camel Trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA

Elwathig¹,

Faye

Thévenon

et al. 2016

Emir. J. Food Agric

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Epidemiological Surveys of camel Trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA

Elwathig¹,

Faye

Thévenon

et al. 2016

Emir. J. Food Agric

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“…The existing serological tests detecting specific antibodies are the Card Agglutination Test for trypanosomes (CATT/T. evansi) and the ELISA using soluble antigens from Whole Cell Lysate (WCL) (OIE terrestrial manual, chapter 2.2.17); the first is targeting immunoglobulins type M (IgM) (early infections) and the last is generally applied to detection of IgG (established infection) (Greiner et al 1997;Reyna-Bello et al 1998;Desquesnes et al 2009;Kumar et al 2013). Conventional parasitological techniques are not sensitive and immunological methods; particularly using locally produced WCL soluble antigens have inherent problems of lack of reproducibility as along with ethical considerations *Corresponding author:yadavsc@rediffmail.com Brought to you by | New York University Bobst Library Technical Services Authenticated Download Date | 10/6/15 10:40 AM (use of laboratory rats for antigen preparation).…”

Section: Introductionmentioning

confidence: 99%

Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids

Kumar

Chaudhury

Bera

et al. 2015

Acta Parasitologica

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The present immuno-diagnostic method using soluble antigens from whole cell lysate antigen for trypanosomosis have certain inherent problems like lack of standardized and reproducible antigens, as well as ethical issues due to in vivo production, that could be alleviated by in vitro production. In the present study we have identified heat shock protein 70 (HSP70) from T. evansi proteome. The nucleotide sequence of T. evansi HSP70 was 2116 bp, which encodes 690 amino acid residues. The phylogenetic analysis of T. evansi HSP70 showed that T. evansi occurred within Trypanosoma clade and is most closely related to T. brucei brucei and T. brucei gambiense, whereas T. congolense HSP70 laid in separate clade. The two partial HSP70 sequences (HSP-1 from N-terminal region and HSP-2 from C-terminal region) were expressed and evaluated as diagnostic antigens using experimentally infected equine serum samples. Both recombinant proteins detected antibody in immunoblot using serum samples from experimental infected donkeys with T. evansi. Recombinant HSP-2 showed comparable antibody response to Whole cell lysate (WCL) antigen in immunoblot and ELISA. The initial results indicated that HSP70 has potential to detect the T. evansi infection and needs further validation on large set of equine serum samples.

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“…However, this result seems not to have been followed up. An ELISA assay for the related parasite Trypanosoma evansi , based on a soluble protein extract, also gave promising results [9] although sensitivity was less than 90% [10]. So far, however, the antigens involved have not been characterized.…”

Section: Introductionmentioning

confidence: 99%

A Search for Trypanosoma brucei rhodesiense Diagnostic Antigens by Proteomic Screening and Targeted Cloning

et al. 2010

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BackgroundThe only available diagnostic method for East African trypanosomiasis is light microscopy of blood samples. A simple immunodiagnostic would greatly aid trypanosomiasis control.Methodology and Principal FindingsTo find trypanosome proteins that are specifically recognised by sera from human sleeping sickness patients, we have screened the Trypanosoma brucei brucei proteome by Western blotting. Using cytosolic, cytoskeletal and glycosomal fractions, we found that the vast majority of abundant trypanosome proteins is not specifically recognised by patient sera. We identified phosphoglycerate kinase (PGKC), heat shock protein (HSP70), and histones H2B and H3 as possible candidate diagnostic antigens. These proteins, plus paraflagellar rod protein 1, rhodesain (a cysteine protease), and an extracellular fragment of the Trypanosoma brucei nucleoside transporter TbNT10, were expressed in E. coli and tested for reactivity with patient and control sera. Only TbHSP70 was preferentially recognized by patient sera, but the sensitivity and specificity were insufficient for use of TbHSP70 alone as a diagnostic. Immunoprecipitation using a native protein extract revealed no specifically reacting proteins.ConclusionsNo abundant T. brucei soluble, glycosomal or cytoskeletal protein is likely to be useful in diagnosis. To find useful diagnostic antigens it will therefore be necessary to use more sophisticated proteomic methods, or to test a very large panel of candidate proteins.

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Antibody-ELISA for Trypanosoma evansi: Application in a serological survey of dairy cattle, Thailand, and validation of a locally produced antigen

Cited by 38 publications

References 26 publications

Epidemiological Surveys of camel Trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA

Epidemiological Surveys of camel Trypanosomosis in Al-jouf, Saudi Arabia based on PCR and ELISA

Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids

A Search for Trypanosoma brucei rhodesiense Diagnostic Antigens by Proteomic Screening and Targeted Cloning

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